CELLMED (셀메드)

Cellmed Orthocellular Medicine and Pharmaceutical Association (셀메드 세포교정의약학회)
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Effect of chloroform extract of traditional Dicranopteris linearis leaves against paracetamol- and CCl4-induced liver toxicity in rats

  • Din, Syafawati Shamsahal (Department of Biomedical Sciences, Faculty of Medicine and Health Science, Universiti Putra Malaysia) ;
  • Mamat, Siti Syariah (Department of Biomedical Sciences, Faculty of Medicine and Health Science, Universiti Putra Malaysia) ;
  • Ismail, Noor Aisyah (Department of Biomedical Sciences, Faculty of Medicine and Health Science, Universiti Putra Malaysia) ;
  • Zainulddin, Wan Noraziemah Wan (Department of Biomedical Sciences, Faculty of Medicine and Health Science, Universiti Putra Malaysia) ;
  • Zabidi, Zalina (Department of Biomedical Sciences, Faculty of Medicine and Health Science, Universiti Putra Malaysia) ;
  • Yahya, Farhana (Department of Biomedical Sciences, Faculty of Medicine and Health Science, Universiti Putra Malaysia) ;
  • Kamisan, Farah Hidayah (Department of Biomedical Sciences, Faculty of Medicine and Health Science, Universiti Putra Malaysia) ;
  • Mohtarrudin, Norhafizah (Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia) ;
  • Othman, Fezah (Department of Biomedical Sciences, Faculty of Medicine and Health Science, Universiti Putra Malaysia) ;
  • Suhaili, Zarizal (Faculty of Agriculture and Biotechnology, Universiti Sultan Zainal Abidin) ;
  • Zakaria, Zainul Amiruddin (Department of Biomedical Sciences, Faculty of Medicine and Health Science, Universiti Putra Malaysia)
  • Received : 2012.06.12
  • Accepted : 2012.10.26
  • Published : 2012.11.30
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Abstract

The present study aimed to determine the hepatoprotective activity of the chloroform extract of D. linearis leaves (CEDL) using the paracetamol (PCM)- and carbon tetrachloride ($CCl_4$)-induced liver injury models in rats. The rats received $dH_2O$ (negative control), 200 mg/kg of silymarin (positive control) or CEDL (50, 250 and 500 mg/kg) orally once daily for 7 days and then were subjected to the hepatotoxic induction on the $7^{th}$ day. The samples (i.e. blood and liver) were collected and underwent biochemical and microscopical analysis, respectively. From the data obtained, both inducers caused significant (p < 0.05) increase in the levels of AST and ALT when compared to the control group, which were significantly (p < 0.05) reduced by CEDL in a generally dose-dependent manner. These biochemical findings were supported by the histopathological analysis and histological scoring. In conclusion, CEDL possesses potential hepatoprotective activity, which could be associated with its flavonoid and tannin contents with the mechanisms of hepatoprotection linked to either its antioxidant or anti-inflammtory/immunomodulating activities. Further in-depth studies are required to identify the responsible bioactive compound.

Keywords

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