Korean Journal of Breeding Science (한국육종학회지)

The Korean Society of Breeding Science (한국육종학회)
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Genetic Diversity of Korean Barley (Hordeum vulgare L.) Varieties Using Microsatellite Markers

Microsatellite 마커를 이용한 한국 보리 품종의 유전적 다양성

  • Kwon, Yong-Sham (Variety Testing Division, Korea Seed & Variety Service, MIFAFF) ;
  • Hong, Jee-Hwa (Variety Testing Division, Korea Seed & Variety Service, MIFAFF) ;
  • Choi, Keun-Jin (Variety Testing Division, Korea Seed & Variety Service, MIFAFF)
  • 권용삼 (농림수산식품부 국립종자원 재배시험과) ;
  • 홍지화 (농림수산식품부 국립종자원 재배시험과) ;
  • 최근진 (농림수산식품부 국립종자원 재배시험과)
  • Received : 2011.09.19
  • Published : 2011.09.30
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Abstract

Microsatellite markers were utilized to investigate genetic diversity among 70 Korean barley varieties (Hordeum vulgare). Ninety nine microsatellite primer pairs were screened for 9 varieties. Twenty primer pairs showed highly polymorphic. The relationship between markers genotypes and 70 varieties was analyzed. A total of 124 polymorphic amplified fragments were obtained by using 20 microsatellite markers. Two to nine SSR alleles were detected for each locus with an average of 6.2 alleles per locus. Average polymorphism information content (PIC) was 0.734, ranging from 0.498 to 0.882. A total of 124 marker loci were used to calculate Jaccard's distance coefficients for cluster analysis using UPGMA. Clustering group was divided 2 groups corresponding to 2-rowed and 6-rowed barley varieties. The phenogram was discriminated all varieties by markers genotypes. These markers may be used wide range of practical application in variety identification and genetic purity assessment of barley.

국내 육성된 보리 품종의 유전적 다양성을 조사하기 위하여 microsatellite marker의 이용 가능성에 대한 연구를 수행하여 얻어진 결과를 요약하면 다음과 같다. 보리 70품종을 20개의 microsatellite marker를 이용하여 분석하였을 때 대립유전자의 수는 2~9개로 비교적 다양한 분포를 나타내었으며 전체 124개의 대립유전자가 분석되었다. PIC 값은 0.498~0.882 범위에 속하였으며 평균값은 0.734로 나타났다. microsatellite marker를 이용하여 작성된 보리70품종의 품종간 유전적 거리는 0.10~0.91의 범위로 나타났고, 유사도 지수 0.15를 기준으로 할 때 70개 품종은 2조 보리 그룹과 6조 보리 그룹으로 나눌 수 있었으며, 공시 품종 모두 microsatellite marker의 genotype에 의해 뚜렷이 구분되었다. 이 연구결과는 보리의 유전적 다양성 및 품종식별을 위한 기초 자료로 유용하게 이용될 수 있는 것으로 사료된다.

Keywords

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