쌀단백질 효소분해물을 이용한 효모추출물의 제조

Preparation of Yeast Extract Using an Enzyme Hydrolysate of Rice Protein

  • 이형진 (한경대학교 식품생물공학과 및 식품생물산업연구소) ;
  • 정하열 (한경대학교 식품생물공학과 및 식품생물산업연구소)
  • Lee, Hyung-Jin (Department of Food Science & Biotechnology and Food & Biotechnology Research Center, Hankyong National University) ;
  • Chung, Ha-Yull (Department of Food Science & Biotechnology and Food & Biotechnology Research Center, Hankyong National University)
  • 투고 : 2011.07.27
  • 심사 : 2011.08.11
  • 발행 : 2011.08.31

초록

쌀단백질을 효소분해하여 제조한 배양액으로 천연조미소재로 사용할 효모추출물을 제조하기 위한 최적 공정조건을 조사하였다. 쌀단백질(5%, w/w)을 단백분해효소 인 $Delvolase^{(R)}$로 효소분해한 상등분획에 3%(w/w) 수준으로 포도당을 첨가한 배지 조건이 가장 적절하였으며 이 조건에서 2.3 g/L의 효모를 회수하였다. 회수한 효모에는 RNA가 188.1 mg/g수준으로 함유되어 있었으며 GMP 및 IMP는 각각 $650.33{\pm}48{\mu}g/g$, $69{\pm}21{\mu}g/g$ 함유되어 있었다. 이와 같은 효모추출물에 효모배양액의 제조 후 남은 쌀단백질 잔사의 효소분해물(Rrh)을 혼합하면 감칠맛이 상승하였는데 미각센서 분석기로 측정한 결과 효모추출물의 감칠맛이 4.88에서 Rrh의 첨가 후에는 9.25로 증가하였으며 관능검사에서도 감칠맛의 증가를 확인할 수 있었다. 이와 같이 효모추출물에 쌀단백질 잔사의 효소분해물이 추가되면 효소분해 과정 중 생성된 다양한 맛 성분으로 인해 감칠맛 이 상승되어 전체적인 기호도가 높아지므로 천연조미소재 로서의 활용이 가능함을 알 수 있었다.

Optimum conditions to prepare a yeast extract with rice protein hydrolysates(rh) were investigated. The yeast extract was obtained at the level of 2.3 g/L from the yeast culture medium($30^{\circ}C$, 48 hr) composed of 5% rh and glucose( 3%, w/w). Within the extract, RNA was contained at the level of 188.1 mg/g and the levels of GMP and IMP as nucleotides were $650.33{\pm}48{\mu}g/g$, $69{\pm}21{\mu}g/g$, respectively. When Rrh(Residual rice protein hydrolysate by $Delvolase^{(R)}$) was supplemented into a yeast extract, the savory taste like umami of the mixture was found to increase noticeably based on the measurements by taste sensing system as well as sensory test. It is assumed that soluble peptides in Rrh could play an important role in improving the overall taste of yeast extract by enhancing its umami taste. Therefore, the yeast extract supplemented with Rrh could be used for manufacturing a high value-added natural seasoning ingredient.

키워드

과제정보

연구 과제 주관 기관 : 농촌진흥청

참고문헌

  1. A.O.A.C. Official Methods of Analysis. 15thed. 1990. Association of Official Analytical Chemists, Washington, DC, USA
  2. Bingham SA. 1990. Mechanisms and experimental and epidemiological evidence relating dietary fibre(non-starch polysaccharides) and starch to protection against large bowel cancer. Proc. Nutr. Soc. 49: 153-71. https://doi.org/10.1079/PNS19900021
  3. Burks, AW, Helm, RM. 1994. Hypoallergenicity of rice protein. Annual meeting of the American Association of Cereal Chemists, Nashville, TN, USA.
  4. Chae HJ, In MJ. 2004. Production of yeast extract by a combined method of autolysis and enzymatic hydrolysis. Korean J. Biotech. Bioeng. 19: 245-249.
  5. Chae JS, Chun HK, Hwang DY, Nam HJ. 2005. Consumer preceptions of food-related hazards and correlates of degree of concerns about food. Korean J. Soc. Food Sci. Nutr. 34: 66-74. https://doi.org/10.3746/jkfn.2005.34.1.066
  6. Crawford GW, Gh Lee. 2003. Agricultural origins in the Korean peninsula. Antiquity 77: 87-95.
  7. Ha TY. 2005. Functionality of rice. In Proceedings of the Korean Society of Food and Cookery Science Conference. May 21, Seoul, Korea. pp. 19-26.
  8. Gabriel AS, Uneyama H, Maekawa T, Torii K. 2009. The calcium- sensing receptor in taste tissue.Biochem. Biophy. Res. Com. 378: 414-418. https://doi.org/10.1016/j.bbrc.2008.11.060
  9. Huang J, Hu R, Rozelle S, Pray C. 2005. Insect-resistant GM rice in farmers' fields: Assessing productivity and health effects in China. Science 308: 688-690. https://doi.org/10.1126/science.1108972
  10. Jeong YJ, Heong KH, Seo JH. 2005. Characteristics of soybean hydrolysates prepared with various protease. Korean J. Food Preserv. 12: 460-464.
  11. Jiaratsatit JIT, Keoplung M, Chumslip. 1987. Glycemic effects of rice and glutinous rice on treated-type II diabetic subjects. J. Med Assoc. Thai. 70: 401-409.
  12. Kim H, Lee JS, Cha YJ. 2002. Processing of functional enzymehydrolyzed sauce from Anchovy sauce and soy sauce processing by-products 1. Optimization of hydrolysis conditions by response surface methodology. J. Koreran Soc. Food Sci. Nutr. 31: 653-657. https://doi.org/10.3746/jkfn.2002.31.4.653
  13. Kim JS, Kim JW, Shim W, Kim JW, Park KH, Pek UH. 1999. Preparation of flavor-enhancing yeast extract using a Saccharomyces cerevisiae strains with high RNA content. Korean J. Food Sci. Tech. 31: 475-481.
  14. Kwok RHM. 1968. The Chinese restaurant syndrome. Letter to the editor. N. Engl. J. Med. 278: 796.
  15. Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685. https://doi.org/10.1038/227680a0
  16. Lee JY, Kim KD. 2009. A study on the perception of and concern for food safety among urban housewives. Korean J. Food Preserv. 16: 999-1007.
  17. Lin CF, Lin CR. 1987. Preparing protein for hydrolysis and product. US Patent. No.4,636,388.
  18. Ogasawara M, Katsumata T, Egi M. 2006. Taste properties of Maillard-reaction products prepared from 1000 to 5000 Da peptide. Food Chem. 99: 600-604. https://doi.org/10.1016/j.foodchem.2005.08.040
  19. Ogasawara M, Yamada Y, Makoto E.2006.Taste enhancer from the long-term ripening of miso (soybean paste). Food Chem. 99: 736-741. https://doi.org/10.1016/j.foodchem.2005.08.051
  20. Ohsu T, Amino Y, Hiroaki N, Yamanaka T, Takeshita S, Hatanaka T, Maruyama Y, Miyamura N, Eto Y. 2010. Involvement of the calcium-sensing receptor in human taste perception. J. Biolog. Chem. 285: 1016-1022. https://doi.org/10.1074/jbc.M109.029165
  21. Park IW, Lee NT, Oh KC. 2008. A study on the development of high functional food protein ingredient and its hydrolyzates from rice bran. Rural Development Administration Report. Suwon, Korea.
  22. Sohn KH, Park HK. 1997. Analysis of significant factor in the flavor of traditional Korean soy sauce. Korean J. Dietary Culture 12: 63-69.
  23. Soldo T, Blank I, Hofmann T. 2003. (+)-(S)-Alapyridaine A general taste enhancer. Chem. Senses 28: 371-379. https://doi.org/10.1093/chemse/28.5.371
  24. Sung NE, Kang HR. 1970. On the amino acid compositions of the Korean cereal proteins. Korean J. Nutr. 3: 113-117.