Taguchi 법에 의한 흑목이버섯의 항산화활성 증진을 위한 열수추출 최적화

Optimization of Hot Water Extraction for Enhancing Antioxidative Activity from Auricularia auricula Using Taguchi Approach

  • 김현민 (강원대학교생물공학과) ;
  • 허원 (강원대학교생물공학과) ;
  • 이신영 (강원대학교생물공학과)
  • Kim, Hyeon-Min (Department of Bioengineering and Technology, Kangwon National University) ;
  • Hur, Won (Department of Bioengineering and Technology, Kangwon National University) ;
  • Lee, Shin-Young (Department of Bioengineering and Technology, Kangwon National University)
  • 투고 : 2011.05.24
  • 심사 : 2011.07.11
  • 발행 : 2011.08.31

초록

Optimization study of the hot water extraction for enhancing antioxidative activity from Auricularia auricula was performed by Taguchi approach using orthogonal matrix $L_9(3^4)$ method. The correlation between DPPH radical scavenging activity and the components of samples extracted from different extraction conditions were also analyzed. The correlation coefficient between DPPH radical scavenging activity and melanin content of A. auricula were 0.93, indicating 'good correlation'. The optimum extraction conditions was obtained at the extraction time of 1 hr. temperature of $85^{\circ}C$, solid: water ratio of 1: 40(w/v) and frequency of 2 times. Under these conditions, values of maximum DPPH free radical scavenging activity and melanin contents of A. auricula were $67.21{\pm}2.17$ and $52.94{\pm}2.10 mg/g$, respectively. Melanin content of 1.6 times and DPPH free radical scavenging effect of 130% were enhanced by optimization.

키워드

참고문헌

  1. Blois MS. 1958. Antioxidant determination by the use of a stable free radical. Nature 26: 1199-1200.
  2. Changa MY, Tsaia GJ, Houng JY. 2006. Optimization of the medium composition for the submerged culture of Ganoderma lucidum by Taguchi array design and steepest ascent method. Enzyme Microb. Tech. 38(3-4): 407-414. https://doi.org/10.1016/j.enzmictec.2005.06.011
  3. Ebarandu AR, Luta G, Edwards JA, McAnalley BH, Davis B. 2005. Quantitative colorimetric analysis of aloe polysaccharides as a measure of Aloe vera quality in commercial products. J. AOAC Inter. 88(3): 684-691.
  4. Eskandari Nasab M, Sam A, Alamdar Milani S. 2011. Determination of optimum process conditions for solvent extraction of thorium using Taguchi method. J. Radioanal Nucl. Chem. 287: 239-245. https://doi.org/10.1007/s10967-010-0857-1
  5. Hou XJ, Chen W. 2008. Optimization of extraction process of crude polysaccharides from wild edible BaChu mushroom by response surface methodology. Carbohyd. Polym. 72: 67-74. https://doi.org/10.1016/j.carbpol.2007.07.034
  6. Kim HM, Hur W, Lee SY. 2009a. Isolation and characterization of dark brownish pigments from fruit body of Auricularia auricula. Food Eng. Progress 13(4): 282-288.
  7. Kim HM, Hur W, Lee SY. 2009b. Composition and structural characteristics of polysaccharide from hot water extraction of Auricularia auricula. KSBB Journal 24: 584-590.
  8. Kim OH, Lim JM, Joo JH, Kim SW, Hwang HJ, Choi JW, Yun JW. 2005. Optimization of submerged culture condition for the production of mycelial biomass and exopolysaccharides by Agrocybe cylindracea Bioresour. Technol. 96: 1175-1182. https://doi.org/10.1016/j.biortech.2004.09.021
  9. Lee SY, Bae HK, Kim NK, Hwang SH. 2008. Optimization of growth conditions of Lentinus edodes mycelium on corn processing waste using response surface analysis. J. Biosci. Bioeng. 105(2): 161-163. https://doi.org/10.1263/jbb.105.161
  10. Li JR, Yin J, Zhu JL, Zhang Q. 2010. Optimization of conditions for ultrasonic-assisted extraction of $\gamma$-glutamyltranspeptidase from Shiitake mushroom by response surface methodology. Food Sci. 31(2): 20-23.
  11. Liu GQ, Zhao Y, Wang XL, Zhu CY. 2011. Response surface methodology for optimization of polysaccharides extraction by mild alkaline hydrolysis from fruiting body of medicinal mushroom, Ganoderma lucidum. JMPR 5(10): 2064-2070.
  12. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265-275.
  13. Montgomery DC. 2001. Design and Analysis of Experiments (5th ed.). John Wiley & Sons, Inc., New York, USA.
  14. Prasad, K.K., Mohan, S.V., Rao, R.S., Pati, B.R.,Sarma, P.N. (2005). Laccase production by Pleurotus ostreatus 1804: optimization of submerged culture conditions by Taguchi DOE methodology. Biochem. Eng. J. 24: 17-26. https://doi.org/10.1016/j.bej.2005.01.019
  15. Rispoli FJ, Shah V. 2007. Mixture design as a first step for optimization of fermentation medium for cutinase production from Colletotrichum lindemuthianum. J. Ind. Microbiol Biotechnol. 34: 349-355. https://doi.org/10.1007/s10295-007-0203-y
  16. Slinkard J, Singleton VL. 1977. Total phenol analysis: automation and comparison with manual method. Am. J. Enol. Vitic. 28: 49-55.
  17. Taguchi, G., 1986. Introduction to Quality Engineering: Designing quality into product and processes. Asian Productivity Organization, Tokyo, Japan.
  18. Wang CC, Sheu SR, Chou YY, Jang MJ, Yang LC. 2011. Determination of optimum process conditions for solvent extraction of thorium using Taguchi method. Thermal Sci. 15(Suppl. 1): S53-S59. https://doi.org/10.2298/TSCI11S1053W
  19. Youn SJ, Cho JG, Kwoen DJ, Choi UK, Kang SC. 2006. Determination of optimal conditions by response surface methodology and quality characteristics of water extracts of Phellinus linteus. Korean Soc. Appl. Biol. Chem. 49(3): 215-220.