한국육종학회지 (Korean Journal of Breeding Science)

  • 제43권5호
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  • Pages.405-412
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  • 2011
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  • 0250-3360(pISSN)
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  • 2287-5174(eISSN)
한국육종학회 (The Korean Society of Breeding Science)
권호 표지

튀김용 옥수수 자식계통들에 대한 유전적 변이성

Analysis of Genetic Variation Among Popcorn Inbred Lines by SSR Markers

  • 장진선 (강원도 농업기술원 옥수수시험장) ;
  • 장은하 (강원도 농업기술원 옥수수시험장) ;
  • 사규진 (강원대학교 농업생명과학대학 식물자원응용공학과) ;
  • 김종화 (강원대학교 농업생명과학대학 원예학과) ;
  • 이주경 (강원대학교 농업생명과학대학 식물자원응용공학과)
  • Jang, Jin-Sun (Maize Experiment Station, Kangwon Agricultural Research and Extension Services) ;
  • Chang, Eun-Ha (Maize Experiment Station, Kangwon Agricultural Research and Extension Services) ;
  • Sa, Kyu-Jin (Department of Applied Plant Sciences, College of Agriculture and life Sciences, Kangwon National University) ;
  • Kim, Jong-Hwa (Department of Horticulture, College of Agriculture and life Sciences, Kangwon National University) ;
  • Lee, Ju Kyong (Department of Applied Plant Sciences, College of Agriculture and life Sciences, Kangwon National University)
  • 투고 : 2011.07.30
  • 심사 : 2011.10.10
  • 발행 : 2011.12.30
⟨ 이전 논문 다음 논문 ⟩

초록

옥수수에서 자식계통들에 대한 유전적 다양성 및 계통유연관계 정보는 1대잡종 품종개발을 위한 교배조합 선발에 유용한 전략을 제공한다. 본 연구는 86개의 튀김 옥수수 자식계통들에 대한 유전적 다양성 및 계통유연관계를 밝히기 위하여 옥수수 전체 게놈을 대표할 수 있도록 50개의 SSR primer를 선발하여 분석에 이용하였다. 그 결과 50개의 SSR primer들은 86개의 튀김 옥수수 자식계통들에서 총 256개의 대립단편을 나타내었으며, SSR loci당 평균 5.1개가 증폭되었다. 각 SSR primer들에서 증폭된 대립단편의 수는 최소 2개에서 최대 16개의 범위로 나타났고, 유전적 다양성 값은 0.210에서 0.831 범위로 나타나 평균 0.579 값을 나타내었다. 계통유연관계 분석에서 86개의 튀김 옥수수 자식계통들은 유전적 유사성 35.8% 수준에서 크게 3개의 Group으로 구분되었는데, Group I은 40계통을, Group II는 39계통을, 그리고 Group III은 7계통을 각각 포함하였다. 본 연구에서 분석에 이용한 SSR 마커들은 우리나라에서 육성한 86개의 튀김 옥수수 자식계통들에 대한 유전적 다양성 및 계통유연관계를 평가하는데 효율적이었음을 나타내었다.

Knowledge of genetic diversity and genetic relationships among inbred lines gives a significant impact on the selection of parental lines for hybrid maize varieties. Genetic diversity and genetic relationships among 86 popcorn inbred lines were analyzed using 50 SSR markers distributed over the whole genome. A total of 256 alleles were identified at all the SSR loci with an average of 5.1 and a range between two and sixteen per locus. The gene diversity values varied from 0.21 to 0.831 with an average of 0.579. The cluster tree generated using the described SSR markers recognized three major groups at 35.8% genetic similarity. Groups I, II, III respectively included 40, 39 and 7 inbred lines. The present study indicates that the SSR markers chosen for this analysis are effective for the assessment of genetic diversity and genetic relationships among 86 popcorn inbred lines in Korea.

키워드

과제정보

연구 과제 주관 기관 : 농촌진흥청

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