한국육종학회지 (Korean Journal of Breeding Science)

  • 제43권5호
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  • Pages.375-382
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  • 2011
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  • 0250-3360(pISSN)
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  • 2287-5174(eISSN)
한국육종학회 (The Korean Society of Breeding Science)
권호 표지

Characterization of a New High-lysine Mutant in Barley (Hordeum vulgare L.)

  • Kim, Hong-Sik (Upland Crop Division, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Dea-Wook (Upland Crop Division, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Sun-Lim (Upland Crop Division, National Institute of Crop Science, Rural Development Administration) ;
  • Baek, Seong-Bum (Upland Crop Division, National Institute of Crop Science, Rural Development Administration) ;
  • Park, Hyoung-Ho (Department of Rice and Winter Cereal Crop, National Institute of Crop Science, Rural Development Administration) ;
  • Hwang, Jong-Jin (Upland Crop Division, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Si-Ju (Upland Crop Division, National Institute of Crop Science, Rural Development Administration)
  • 투고 : 2011.10.19
  • 심사 : 2011.12.12
  • 발행 : 2011.12.30
⟨ 이전 논문 다음 논문 ⟩

초록

A chemical, MNU-induced hulless barley mutant line designated as 'Mutant 98 (M98)' was developed from a Korean hulless waxy barley cultivar, 'Chalssalbori'. The objective of the study was to determine the genetic basis of 'M98' and the possibility of using 'M98' as breeding parent to improve lysine level. Compared to 'Chalssalbori', 'M98' had large embryo and higher lysine content in both the embryo and endosperm. Significantly different lysine content in 'M98' and the other high-lysine barley mutant stocks was observed for two years. However, the genotype by year interaction was not significant. 'M98' was higher than the other high-lysine barley mutant stocks in the percentage of lysine of total amino acid composition (0.75%). The trait of shrunken endosperm of 'M98', which was typical in the high-lysine mutants, was inherited by a single recessive gene. Based on seed morphology and lysine content of $F_1$ seeds, 'M98' had a genetically different gene from the other high-lysine mutants for shrunken endosperm. Segregation of $F_2$ for plump/shrunken endosperm did not fit the expected ratio of Mendelian inheritance except for only one cross combination (GSHO1784 (lys1)/M98). The amino acid analysis of $F_5$ and $F_6$ progenies from the cross between 'M98' and 'Chalssalbori' revealed that the attempt to increase the range of lysine content of plump lines did not go beyond the limit of the average high-lysine barley germplasm.

키워드

과제정보

연구 과제 주관 기관 : International Atomic Energy Agency

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