Characterization as Cosmetic Substances of Chestnut Inner Skin Extracts with Antioxidant Activity

항산화 활성을 갖는 율피 추출물의 향장 소재 특성

  • Jeong, Hee-Rok (Dept. of Food Science & Technology, Gyeongsang National Univ.(Insti. of Agric. & Life Sci.)) ;
  • Kim, Ji-Hye (Dept. of Food Science & Technology, Gyeongsang National Univ.(Insti. of Agric. & Life Sci.)) ;
  • Jo, Yu-Na (Dept. of Food Science & Technology, Gyeongsang National Univ.(Insti. of Agric. & Life Sci.)) ;
  • Jeong, Ji-Hee (Dept. of Food Science & Technology, Gyeongsang National Univ.(Insti. of Agric. & Life Sci.)) ;
  • Heo, Ho Jin (Dept. of Food Science & Technology, Gyeongsang National Univ.(Insti. of Agric. & Life Sci.))
  • 정희록 (경상대학교 식품공학과(농업생명과학연구원)) ;
  • 김지혜 (경상대학교 식품공학과(농업생명과학연구원)) ;
  • 조유나 (경상대학교 식품공학과(농업생명과학연구원)) ;
  • 정지희 (경상대학교 식품공학과(농업생명과학연구원)) ;
  • 허호진 (경상대학교 식품공학과(농업생명과학연구원))
  • Received : 2011.09.29
  • Accepted : 2011.12.20
  • Published : 2011.12.30

Abstract

We investigated characterization as cosmetic substances of chestnut inner skin extracts with in vitro antioxidant activity. Total phenolics of various extracts from chestnut inner skin were the highest 60% methanol (164.82 mg/g), and ethyl acetate fractions (191.14 mg/g). We found that the both samples from chestnut inner skin dose-dependently increased in vitro antioxidant activities (DPPH radical scavenging activity and inhibition of lipid peroxidation). In addition, the both samples also showed a strong UV absorption in the range of UV-B (290-320 nm). Especially the 60% ethanol extracts presented higher inhibitory effect on elastase (46.40% at $100{\mu}g/mL$) than that of the ethyl acetate fractions, so that it showed in vitro anti-wrinkle activity. Finally, the 60% methanol extracts and ethyl acetate fractions showed anti-bacterial activity against skin pathogenic bacteria. Consequently, these results suggest that the chestnut inner skin can be used for cosmetic industry.

In vitro 항산화 활성을 갖는 율피 추출물의 향장 소재 특성을 조사하였다. 율피로부터의 다양한 추출물 중 총 페놀성 화합물 함량은 60% 메탄올 추출물 및 에틸아세테이트 분획물이 각각 164.82 및 191.14 mg/g으로 가장 높은 것으로 나타났다. 또한, 상기 두 분획물에 의한 in vitro 항산화 활성(DPPH radical scavenging activity, lipid peroxidation 저해)은 농도 의존적으로 증가한다는 것을 확인하였다. 상기 두 분획물은 UV-B(290-320 nm) 영역에서 강한 UV 흡수도를 보여주었다. 특히, 율피 유래 두 분획물은 $1000{\mu}g/mL$에서 42.88 및 33.02%의 elastase 저해 활성을 나타내어 우수한 주름 개선 특성을 가지는 것을 보여주었다. 아울러, 율피로부터의 60% 메탄올 추출물 및 에틸아세테이트 분획물은 피부 상재균에 대하여서도 항균 활성을 지니는 것으로 나타났다. 이러한 결과들은 율피에서 추출될 수 있는 물질이 향장 산업소재로서 활용될 수 있는 가능성을 제시하는 것으로 판단된다.

Keywords

Acknowledgement

Supported by : 한국연구재단, 지식경제부

References

  1. Kang, D. Y., M. O. Shin, J. H. Son, and S. J. Bae. 2009. The antioxidative and antimicrobial effects of Celastrus orbiculatus. J. Life Sci. 19: 52-57. https://doi.org/10.5352/JLS.2009.19.1.052
  2. Zhoh, C. K., B. N. Kim, S. H. Hong, and C. G. Han. 2002. The antimicrobial effects of natural aromas for substitution of parabens. J. Soc. Cosme. Scientists Korea, 28: 166-185.
  3. Shimizu, K., R. Kondo, K. Sakai, N. Takeda, and T. Nagahata. 2002. The skin-lightening effects of artocarpin on UVB-induced pigmentation. Planta Med. 68: 79-81. https://doi.org/10.1055/s-2002-20057
  4. Black, D. L., R. Chatterjee, and D. P. Hannon. 1991. Chronic ultraviolet radiation-induced increase in skin iron and the photoprotective effect of topically applied iron chelators. Photochem. Photobio. 54: 215-223. https://doi.org/10.1111/j.1751-1097.1991.tb02009.x
  5. Burton, G. W. and K. U. Ingold. 1986. Vitamin E: application of the principles of physics organic chemistry to the exploration of its structure and function. Acc. Chem. Res. 19: 194-201. https://doi.org/10.1021/ar00127a001
  6. Cho, S. H., Y. J. Choi, C. W. Rho, C. Y. Choi, D. S. Kim, and S. H. Cho. 2008. Reactive oxygen species and cytotoxicity of bamboo (Phyllostachys pubescens) sap. Korean J. Food Preserv. 15: 105-110.
  7. Ham, S. S., D. H. Oh, J. K. Hong, and J. H. Lee. 1997. Antimutagenic effects of juices from edible Korean wild herbs. J. Food Sci. Nutr. 2: 155-161.
  8. Jun, S., K. Goto, F. Nanjo, S. Kawai, and K. Murata. 2000. Antifungal activity of plant extract againung Arthrinium sacchari and Chaetomium funicola. J. Biosci. Bioeng. 90: 442-446.
  9. Yang, H. J., Y. J. Ahn, J. H. Kim, and S. N. Park. 2008. Antioxidative activity and component analysis of Quercus glauca leaf extracts. J. Soc. Cosmet. Sci. 34: 189-200.
  10. Hong, E. S., G. W. Ahn, and B. K. Jo. 2008. The study on the potential anti-aging properties of Prunella vulgaris extracts in vitro and in vivo. J. Soc. Cosmet. Sci. 34: 129-135.
  11. Yagi, A., T. Kanbara, and N. Morinobu. 1986. The effect of tyrosinase inhibition for aloe. Planta Med. 3981: 517-519.
  12. Yoon, W. J. 1997. The study on the hemidity preserving effect with several natural packs. Dongduk womens University, Seoul, Korea.
  13. Kim, D. O., S. W. Jeong, and C. Y. Lee. 2003. Antioxidant capacity of phenolic phytochemical from various cultivars of plums. Food Chem. 81: 321- 326. https://doi.org/10.1016/S0308-8146(02)00423-5
  14. Kim, D. O., K. W. Lee, H. J. Lee, and C. Y. Lee. 2002. Vitamin C equivalent antioxidant capacity (VCEAC) of phenolic phytochemicals. J. Agric. Food Chem. 50: 3713-3717. https://doi.org/10.1021/jf020071c
  15. Chang, S. T., J. H. Wu, S. Y. Wang, P. L. Kang, N. S. Yang, and L. F. Shyur. 2001. Antioxidant activity of extracts from Acacia confusa bark and heartwood. J. Agric. Food Chem. 49: 3420-3424. https://doi.org/10.1021/jf0100907
  16. Matsuda, H., M. Higashio, Y. Nakai, M. Iinuma, M. Kubo, and L. Frank. 1996. Studies of cuticle drugs from natural sources. IV. Inhibitory effects of some Arctostaphylos plants on melanin biosynthesis. Biol. pharm. Bull. 19: 153-156. https://doi.org/10.1248/bpb.19.153
  17. Kim, B. Y. 2010. Development of functional cosmetic agent from porcine placenta. Master Thesis, Inje University, Gimhae, Korea.
  18. Kim, N. R., Y. H. Lim, S. W. Park, and E. S. Nam. 2009. Antimicrobal activities of the anti-acne compounds from natural sources. Kor. J. Microbial. Biotechnol. 37: 80-84.
  19. Shin, S. J., S. K. Kwon, K. H. Lee, N. D. Sung, and W. Y. Chio. 1994. Extraction and characterization of antibacterial components from the roots of evening primrose(Onenothera odarata Jacquin). J. Agric. Sci. 21: 54-59.
  20. Lee, S. J., K. W. An, T. S. Choi, H. S. Jung, J. H. Moon, and K. H. Park. 2010. Component analysis and antioxidative activity of Castanopsis cuspidata var. sieboldii nut. Kor. J. Food Preserv. 17: 139-144.
  21. Kim M. J., H. G. Yang, and S. N. Park. 2011. Antioxidative activities and antiaging effects of Geum aleppicum Jacq. extracts. J. Soc. Cosmet. Scientists Korea. 37: 191-198.
  22. Lee, H. J., M. J. Chung, J. Y. Cho, S. S. Ham, and M. Choe. 2008. Antioxidative and macrophage phagocytic activities and functional component analyses of selected korean chestnut (Castanea crenata S. et Z.) cultivars. J. Korean Soc. Food Sci. Nutr. 37: 1095-1100. https://doi.org/10.3746/jkfn.2008.37.9.1095
  23. Uchida, K., and E. R. Stadtman. 1993. Covalent attachment of 4-hydroxynonenal to glyceraldehyde-3-phosphate dehydrogenase. A possible involvement of intra and intermolecular cross-linking reaction. J. Biol. Chem. 268: 6388-6393.
  24. Rhie, S. J. 2007. The effect of lavender oil on the activity of antioxidant enzymes to ultraviolet-damaged skin. J. Korean Soc. Cosm. 13: 467-474.
  25. Hwang, E. Y., Y. H. Kong, Y. C. Lee, Y. C. Kim, K. M. Yoo, Y. O. Jo, and S. Y. Choi. 2006. Comparison of phenolic compounds contents between white and red ginseng and their inhibitory effect on melanin biosynthesis. J. Ginseng Res. 30: 82-87. https://doi.org/10.5142/JGR.2006.30.2.082
  26. Kligman, D. 2000. Cosmeceuticals. Dermatol. Clin. 18: 609-615. https://doi.org/10.1016/S0733-8635(05)70211-4
  27. Jang, M. J., S. J. Cheon, H. Y. Kim, D. J. Kwoen, H. Y. Kim, S. H. Kim, and J. T. Lee. 2011. The anti-wrinkle and whitening effect of extracts of Castanea crenata inner shell. J. Life Sci. 21: 734-738. https://doi.org/10.5352/JLS.2011.21.5.734
  28. Kim, J. E., E. H. Kim, and S. N. Par. 2010. Antibacterial activity of Persicaria hydropiper extracts and its application for cosmetic material. Kor. J. Microbial. Biotechnol. 38: 112-115.