한국동물위생학회지 (Korean Journal of Veterinary Service)

  • 제34권3호
  • /
  • Pages.201-208
  • /
  • 2011
  • /
  • 3022-7372(pISSN)
  • /
  • 2287-7630(eISSN)
한국동물위생학회 (The Korean Society of Veterinary Service)
권호 표지
DOI QR코드

DOI QR Code

The biochemical and molecular characteristics of Streptococcus equi subsp. zooepidemicus isolated from the genital tract of Thoroughbred mares in Korea

  • Choi, Seong-Kyoon (College of Verterinary Medicine and Institute of Equine Medicine, Kyungpook National University) ;
  • Kim, Seong-Guk (Gyeongbuk Veterinary Service Laboratory) ;
  • Cho, Gil-Jae (College of Verterinary Medicine and Institute of Equine Medicine, Kyungpook National University)
  • 투고 : 2011.04.01
  • 심사 : 2011.08.26
  • 발행 : 2011.09.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Streptococcus equi subsp. zooepidemicus (S. zooepidemicus) is a pathogen of a variety of infections in horse. We studied biochemical and molecular characteristics of S. zooepidemicus isolated from the genital tract of Thoroughbred mares in Korea. Seventy-nine isolates were identified as S. zooepidemicus by biochemical and PCR method from 374 horses. The biochemical characteristics of S. zooepidemicus isolates were positive reaction of lactose and sorbitol. However, S. zooepidemicus isoltes were negative reaction of inulin, mannitol, raffinose, trehalose, aesculin hydrolysis, growth in 6.5% NaCl and variable reaction of maltose. Epidemiological investigations of S. zooepidemicus isolates were performed by fragment analysis of SzP (S. zooepidemicus protective protein) gene, CNE (collagen binding protein) gene and ISR (16s rRNA intergenic spacer region) gene using ABI Prism $3,130{\times}1$ Genetic Analyzer System. All isolates were shown single amplification size of 906 bp in CNE gene, but SzP and ISR gene were shown variable patterns of fragment size. The characteristics of S. zooepidemicus investigated in this study will be very useful for the prevention of infection and the studies of epidemiologic characteristics of S. zooepidemicus, causing the severe economic losses due to reproductive failures.

키워드

참고문헌

  1. Anzai T, Timoney JF, Kuwamoto T, Wada R, Oikawa M, Higuchi T. 2002. Polymerase chain reaction restriction fragment length polymorphism analysis of the SzP gene of Streptococcus zooepidemicus isolated from the respiratory tract of horses. Am J Vet Res 63: 1298-1301 https://doi.org/10.2460/ajvr.2002.63.1298
  2. Asbury AC, Halliwell RE, Foster GW, Longino SJ. 1980. Immunoglobulin in uterine secretion of mares with differing resistance to endometritis. Therigenology 14: 299-308. https://doi.org/10.1016/0093-691X(80)90079-5
  3. Bain AM. 1966. The role of infection in infertility in the thoroughbred mare. Vet Rec 78: 168-173. https://doi.org/10.1136/vr.78.5.168
  4. Bannerman DD, Paape MJ, Hare WR, Sohn EJ. 2003. Increased levels of LPS-binding protein in bovine blood and milk following bacterial lipopolysaccharide challenge. J Dairy Sci 86: 3128-3137 https://doi.org/10.3168/jds.S0022-0302(03)73914-9
  5. Bienert A, Bartmann CP, Verspohl J, Deegen E. 2003. Bacteriological findings for endodontical and apical molar dental diseases in the horse. Dtsch Tierarztl Wochenschr 110: 358-361.
  6. Cho JJ, Kang SC, Yang HS, Yang JH, Son, WG, Bae JH, Kim JH, 2007. Peritonitis associated with Streptococcus equi subsp. zooepidemicus in a thoroughbred horse. Korean J Vet Res 47: 315-319
  7. Choi SK, Lee SG, Yang JH, Cho GJ. 2007. Distribution and antimicrobial susceptibility patterns of bacteria isolated from genital tract in Thoroughbred mares. J Vet Clin 24: 19-25.
  8. Eigner U, Schmid A, Wild U, Bertsch D, Fahr AM. 2005. Analysis of the comparative workflow and performance characteristics of the VITEK 2 and Phoenix systems. J Clin Microbiol 43: 3829-3834 https://doi.org/10.1128/JCM.43.8.3829-3834.2005
  9. Frontoso R, De Carlo E, Pasolini MP, van der Meulen K, Pagnini U, Iovane G, De Martino L. 2008. Retrospective study of bacterial isolates and their antimicrobial susceptibilities in equine uterine during fertility problems. Res Vet Sci 84: 1-6. https://doi.org/10.1016/j.rvsc.2007.02.008
  10. Harkness JE, Wagner JE. 1983. The biology and medicine of rabbits and rodents. 2nd ed. pp. 173-175. Lea and Febiger, Philadelphia.
  11. Hirota KI, Kakoi H, Gawahara H, Hasegawa T, Tozaki T. 2010. Construction and validation of parentage testing for thoroughbred horses by 53 single nucleotide polymorphisms. J Vet Med Sci 72: 719-726. https://doi.org/10.1292/jvms.09-0486
  12. Hyytia-Trees E, Lafon P, Vauterin P, Ribot EM. 2010. Multilaboratory validation study of standardized multiple-locus variable-number tandem repeat analysis protocol for shiga toxin-producing Escherichia coli O157: a novel approach to normalize fragment size data between capillary electrophoresis platforms. Foodborne Pathog Dis 7: 129-136. https://doi.org/10.1089/fpd.2009.0371
  13. Jeffcott LB, Rossdale PD, Freestone J, Frank CJ, Towers-Clark PF. 1982. An assessment of wastage in thoroughbred racing from conception to 4 years of age. Equine Vet J 14:185-198. https://doi.org/10.1111/j.2042-3306.1982.tb02389.x
  14. Lee SY, Cho GJ. 2006. Parentage testing of Thoroughbred horse in Korea using microsatellite DNA typing. J Vet Sci 7: 63-67. https://doi.org/10.4142/jvs.2006.7.1.63
  15. Lindstedt BA, Torpdahl M, Nielsen EM, Vardund T, Aas L, Kapperud G. 2007. Harmonization of the multiple-locus variable-number tandem repeat analysis method between Denmark and Norway for typing Salmonella Typhimurium isolates and closer examination of the VNTR loci. J Appl Microbiol 102: 728-735. https://doi.org/10.1111/j.1365-2672.2006.03134.x
  16. McKinnon AO, Voss JL, 1993. Equine reproduction. pp. 246-253. Lea & Rebiger, Philadephia
  17. Miragaya MH, Woods GL, Losinno L. 1997. Endometritis, salpingitis and fertilisation rates after mating mares with a history of intrauterine lumenal fluid accumulation. Equine Vet J Suppl 25: 109-112.
  18. Ozgur NY, Ikiz S, Carioglu B, Kilicarslan R, Yilmaz H, Akay O, Ilgaz A. 2001. Contagious equine metritis in Turkey: first isolation of Taylorella equigenitalis from mares. Vet Rec 149: 120-122. https://doi.org/10.1136/vr.149.4.120
  19. Pycock JF, Allen WE. 1990. Inflammatory components in uterine fluid from mares with experimentally induced bacterial endometritis. Equine Vet J 22: 422-425. https://doi.org/10.1111/j.2042-3306.1990.tb04309.x
  20. Quinn PJ, Carter ME, Markey B, Carter GR. 1993. Clinical Veterinary Microbiology. 9th ed. pp. 127-136. Mosby, London
  21. Rober SJ. 1971. Veterinary obstetrics and genital disease. 2nd ed. pp. 135-136. Theriogenology Edward bros.
  22. Roszel JF, Freeman KP. 1988. Equine endometrial cytology. Vet Clin North Am Equine Pract 4: 247-262. https://doi.org/10.1016/S0749-0739(17)30640-5
  23. Welsh RD. 1984. The siginificance of Streptococcus zooepidemicus in the horse. Equine practice 6: 6-16.
  24. Widders PR, Stokes CR, David JS, Bourne FJ. 1984. Quantitation of the immunoglobulins in reproductive tract secretions of the mare. Res Vet Sci 37: 324-330.
  25. Widders PR, Stokes CR, David JS, Bourne FJ. 1985. Effect of cycle stage on immunoglobulin concentrations in reproductive tract secretions of the mare. J Reprod Immunol 7: 233-242. https://doi.org/10.1016/0165-0378(85)90054-3
  26. Wingfield Digby NJ, Ricketts SW. 1982. Results of concurrent bacteriological and cytological examinations of the endometrium of mares in routine stud farm practice 1978-1981. J Reprod Fertil Suppl 32: 181-185.
  27. Younan M, Estoepangestie AT, Cengiz M, Alber J, El-Sayed A, Lammler C. 2005. Identification and molecular characterization of Streptococcus equi subsp. zooepidemicus isolated from camels (Camelus dromedarius) and camel milk in Kenya and Somalia. J Vet Med B Infect Dis Vet Public Health 52: 142-146. https://doi.org/10.1111/j.1439-0450.2005.00828.x

피인용 문헌

  1. A case of meningitis caused by Streptococcus equi subsp. zooepidemicus infection in a foal in Jeju vol.37, pp.1, 2014, https://doi.org/10.7853/kjvs.2014.37.1.67