The Alleviative Effect of White Tea Water Extract on Inflammation and Skin Barrier Damage

백차 열수추출물의 자외선 조사에 의한 피부염증 및 피부장벽손상 완화 효과

  • 이경옥 (호원대학교 미용예술학과) ;
  • 김영철 (계명대학교 대학원 공중보건학과) ;
  • 장병수 (한서대학교 보건학부 피부미용학과)
  • Received : 2011.08.16
  • Accepted : 2011.09.22
  • Published : 2011.09.30

Abstract

To investigate the alleviative effect of white tea water extract on the inflammation and skin barrier damage, skin aging animal model was produced by the irradiation of UVB to the backs of hairless mice for 12 weeks. And then experimental materials were applied topically for 4 weeks. At the 28th day of experiment, positive control (PC, 0.01% retinoic acid treatment) and experimental groups (E1, 1% white tea water extract treatment; E2, 2% white tea water extract treatment) had significantly (p<0.001) lower values of both skin erythema index and transepidermal water loss (TEWL) than the control (C, saline treatment) group. The appearance of mast cell and the degree of its degranulation in dermal and subcutaneous layers were remarkably reduced in E1 and E2 groups compared to the C group. It is found that white tea water extract is effective in skin barrier damage and inflammation in hairless mouse.

백차 열수추출물의 염증 및 피부장벽 손상완화 효과를 알아보기 위해 생후 6~7주령 암컷 SKH-1 hairless 마우스 등 부위에UVB를 12주간 조사하여 피부노화를 유발시킨 후, 실험동물을정상군(N), 대조군(UVB+saline, C), 양성대조군(UVB+0.01%retinoic acid, PC), 실험군1 (UVB+1% white tea water extract, E1), 실험군2 (UVB+2% white tea water extract, E2)로 나누어 1일 2회, 주 5일, 4주 동안 매회 200 ${\mu}L$씩 시료를 도포하여 다음과 같은 결과를 얻었다. (1) 피부 홍반도 측정에서, 실험 4주 후 C군에비해 PC, E1, E2군은 각각 31, 41, 46% 유의하게(p<0.001) 낮았다. (2) 수분함량 측정에서, 실험 4주 후 C군에 비해 PC, E1, E2군은 각각 190, 207, 225% 유의하게(p<0.001) 높았다. 경표피수분 손실량(transepidermal water loss, TEWL) 측정에서, 실험 4주후 C군에 비해 PC, E1, E2군은 각각 61, 79, 85% 유의하게(p<0.001) 낮았다. 조직학적 관찰에서, C군에 비해 E1, E2군은 표피층과 진피층의 비후, 비만세포수와 탈과립 정도가 현저하게 경감되었다. 이상의 연구결과를 종합할 때, hairless 마우스를 이용한in vivo 실험에서 백차 열수추출물 경피 도포는 자외선 조사로 인해 유발된 피부장벽 손상 및 염증 반응에 상당한 개선 효과가 있음을 확인하였다.

Keywords

References

  1. Camouse MM, Domingo DS, Swain FR, Conrad EF, Matsui MS, Maes D, Declercq L, Cooper KD, Stevens SR, Baron ED: Topical application of green and white tea extracts provides protection from solar-simulated ultraviolet light in human skin. Exp Dermatol 18(6) : 522-526, 2009. https://doi.org/10.1111/j.1600-0625.2008.00818.x
  2. Cerutti PA: Oxidant stress and carcinogenesis. Eur J Clin Invest 21 : 1-5, 1991. https://doi.org/10.1111/j.1365-2362.1991.tb01350.x
  3. Chaqour B, Seite S, Coutant K, Foyrtanier A, Borel JP, Bellon G: Chronic UVB- and all-trans retinoic-acid-induced qualitative and quantitative changes in hairless mouse skin. J Photochem Photobiol 28(2) : 125-135, 1995. https://doi.org/10.1016/1011-1344(94)07080-8
  4. Chiba K, Kawakami K, Sone T, Onoue M: Characteristics of skin wrinkling and dermal changes induced by repeated application of squalene monohydroperoxide to hairless mouse skin. Skin Pharmacol Appl Skin Physiol 16(4) : 242-251, 2003. https://doi.org/10.1159/000070847
  5. Choi OS, Kwon MH, Kong MK, Lee SH, Gang SR, Kim PS, Kim YC: Inhibition effects of frankincense oil on skin aging (II). J Environ Toxicol 23(2) : 129-138, 2008. (Korean)
  6. Dona M, Isabella DA, Fiorella C, Roberto B, Monica M, Adriana A, Spiridione G: Neutrophil restraint by green tea: inhibition of inflammation, associated angiogenesis, and pulmonary fibrosis. J Immunol 170 : 4335-4341, 2003. https://doi.org/10.4049/jimmunol.170.8.4335
  7. Fisher GJ, Wang ZQ, Datta SC, Varani J, Kang S, Voorhees JJ: Pathophysiology of premature skin aging induced by ultraviolet light. N Engl J Med 337(20) : 1419-1428, 1997. https://doi.org/10.1056/NEJM199711133372003
  8. Frei B, Jane VH: Antioxidant activity of tea polyphenols in vivo: evidence from animal. Am Soc Nutr Sci 133(10) : 3275S-3284S, 2003.
  9. Ghadially R, Brown BE, Sequeira-Martin SM, Feingold KR, Elias PM: The aged epidermal permeability barrier: Structural, functional, and lipid biochemical abnormalities in humans and a senescent murine model. Clin Invest 95(5) : 2281-2290, 1995. https://doi.org/10.1172/JCI117919
  10. Heinrich U, Neukam K, Tronnier H, Sies H, Stahl W: Long-term ingestion of high flavanol cocoa provides photoprotection against UV-induced erythema and improves skin condition in women. J Nutr 136 : 1565-1569, 2006. https://doi.org/10.1093/jn/136.6.1565
  11. Katiyar SK, Afaq F, Perez A, Mukhtar H: Green tea polyphenol (-)-epigallocatechin-3-gallate treatment of human skin inhibits ultraviolet radiation-induced oxidative stress. Carcinog 22(2) : 287-294, 2001. https://doi.org/10.1093/carcin/22.2.287
  12. Katiyar SK, Perez A, Mukhtar H: Green tea polyphenol treatment to human skin prevents formation of ultraviolet light B-induced pyrimidine dimers in DNA. Clin Cancer Res 6 : 3864-3869, 2000.
  13. Kim J, Hwang JS, Cho YK, Han Y, Jeon YJ, Yang KH: Protective effects of (-)-epigallocatechin-3-gallate on UVA- and UVB-induced skin damage. Skin Pharmacol Appl Skin Physiol 14 : 11-19, 2001. https://doi.org/10.1159/000056329
  14. Kondo S: The roles of cytokines in photoaging. J Dermatol Sci 23(1) : S30-S36, 2000. https://doi.org/10.1016/S0923-1811(99)00076-6
  15. Kown MH, Choi SY, Kim YC: Inhibition effects of Peonia japonica water extract on skin aging (II). J Environ Toxicol 24(2) : 169-178, 2009. (Korean)
  16. Matsumura Y, Ananthaswamy HN: Toxic effects of ultraviolet radiation on the skin. Toxicol Appl Pharmacol 19(3) : 298-308, 2004.
  17. Mukherjee S, Abhijit D, Vandana P, Orting HC, Roeder A, Weindl G: Retinoids in the treatment of skin aging: an overview of clinical efficacy and safety. Clin Interv Aging 1(4) : 327-348, 2006. https://doi.org/10.2147/ciia.2006.1.4.327
  18. Park EY, Kim YC: Effectiveness of white tea extract on skin whitening using in vitro test. J Kor Soc Cosm 15(2) : 611-617, 2009. (Korean)
  19. Pitta PG: Flavonoids as antioxidants. J Nat 63 : 1035-1042, 2000. https://doi.org/10.1021/np9904509
  20. Puch F, Samson-Villeger S, Guyonnet D, Blachon JL, Rawlings AV, Lassel T: Consumption of functional fermented milk containing borage oil, green tea and vitamin E enhances skin barrier function. Exp Dermatol 17(8) : 668-674, 2008. https://doi.org/10.1111/j.1600-0625.2007.00688.x
  21. Shahidi F: Antioxidants in food and food antioxidants. Nahr 44(3) : 158-163, 2000. https://doi.org/10.1002/1521-3803(20000501)44:3<158::AID-FOOD158>3.0.CO;2-L
  22. Shirley BW: Flavonoid biosynthesis: 'new' functions for an 'old' pathway. Trends Plant Sci 11(1) : 377-382, 1996.
  23. Suck TS, Choi SY, Kim YC: Inhibition effects of LED light irradiation on the wrinkle formation in hairless mouse. J Cosmetic Sci 6(4) : 347-356, 2010. (Korean)
  24. Svobodova A, Psotova J, Walterova D: Natural phenolics in the prevention of UV-induced skin damage a review. Biomed Papers 147(2) : 137-145, 2003. https://doi.org/10.5507/bp.2003.019
  25. Varani J, Gendimenico GJ, Shah B, Gibbs D, Capetola RJ, Mezick JA: A direct comparison of pharmacologic effects of retinoids on skin cells in vitro and in vivo. Skin Pharmacol Physiol 4(4) : 254-261, 1991. https://doi.org/10.1159/000210959
  26. Wang ZY, Agarwal R, Bickers RD, Mukhtar H: Protection against ultraviolet B radiation-induced photocarcinogenesis in hairless mice by green tea polyphenols. Carcinog 12(8) : 1527-1530, 1991. https://doi.org/10.1093/carcin/12.8.1527
  27. Zhaorigetu S, Yanaka N, Sasaki M, Watanabe H, Kato N: Inhibitory effects of silk protein, sericin on UVB-induced acute damage and tumor promotion by reducing oxidative stress in the skin of hairless mouse. J Photochem Photobiol B Biol 71 : 11-17, 2003. https://doi.org/10.1016/S1011-1344(03)00092-7