Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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Structure of Egg Envelope and Oogenesis of Kichulchoia multifasciata (Pisces, Cobitidae)

수수미꾸리 Kichulchoia multifasciata의 난자형성과정과 난막의 구조

  • Kim, Chi-Hong (Central Inland Fisheries Research Institute, NFRDI) ;
  • Kim, Jae-Goo (Faculty of Biological Science and Institute for Biodiversity Research, College of Natural Sciences, Chonbuk National University) ;
  • Park, Jong-Young (Faculty of Biological Science and Institute for Biodiversity Research, College of Natural Sciences, Chonbuk National University)
  • 김치홍 (국립수산과학원 중앙내수면연구소) ;
  • 김재구 (전북대학교 자연과학대학 생물학과, 전북대학교 생물다양성연구소) ;
  • 박종영 (전북대학교 자연과학대학 생물학과, 전북대학교 생물다양성연구소)
  • Received : 2011.08.10
  • Accepted : 2011.09.25
  • Published : 2011.09.30
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Abstract

Histological study on egg envelope and oogenesis of Kichulchoia multifasciata (Pisces, Cobitidae) was carried out by light microscopes and scanning electron microscopes. Various developmental cells appeared in ovary of the specimen catched during November 2010. The cytoplasm of oogonia was acidic and many nucleoli were located at the inner side of nucleus membrane. The size of the oogonia was $103.9{\pm}24.7$ ${\mu}m$ with nucleus size $42.9{\pm}6.9$ (31.1~50.3) ${\mu}m$. Primary oocyte having $277.5{\pm}60.5$ (216.7~354.9) ${\mu}m$ in diameter began to accumulate yolk vesicles. As the developmental stages proceed, secondary oocyte grows larger to $617.6{\pm}85.1$ (503.4~723.6) ${\mu}m$, and eosinophilic yolk granules yolk granules appeared between the yolk vesicles occupying most cytoplasm, and there are some yolk mass formed already. There are some yolk mass formed already. Envelope of fertilized egg investigated by a scanning electron microscope had plenty of microvilli (2~3 ${\mu}m$ in length) over the entire egg surface and a micropyle. Especially, the microvilli surrounding the micropyle were longer than those of egg surface with $5.26{\pm}1.22$ ${\mu}m$.

미꾸리과 (Cobitidae)에 속하는 한국 고유종인 수수미꾸리 Kichulchoia multifasciata 생식세포들의 형태학적 특징을 연구하기 위해 조직학적 방법으로 난소 내의 난세포 및 난막의 구조를 광학현미경과 전자현미경을 이용하여 조사하였다. 2010년 11월 수수미꾸리의 난소에는 다양한 발생단계의 생식세포들을 가지고 있었다. 난원세포의 핵은 평균 $42.9{\pm}6.9$ (31.1~50.3) ${\mu}m$이며, 세포 크기는 평균 $103.9{\pm}24.7$ (69.8~130.1) ${\mu}m$로 나타났다. 핵 내 에는 인이 산재하였으며, 전체적으로 hematoxylin에 강하게 염색되었다. 제1난모세포의 핵은 난원세포의 핵보다 커졌으며, 핵 내 의 인들이 핵막 인근으로 이동하여 분포하였고 세포는 평균 $277.5{\pm}60.5$ (216.7~354.9) ${\mu}m$였다. 제1난모세포 성숙 초기에는 세포질에 난황포를 형성하기 시작하였으며 이후 난막의 방사대가 형성되기 시작하였다. 성숙단계가 진행될수록 평균 $617.6{\pm}85.1$ (503.4~723.6) ${\mu}m$의 크기에 제2난모세포가 발달하였고 핵막과 인을 제외한 부분이 호염성 염색물질인 eosin에 강하게 염색되었다. 또한 세포질의 대부분을 차지하고 있는 난황포에는 난황구가 축적되기 시작하였으며 일부 난황괴가 형성되고 있었다. 한편 난막에는 미세융모가 관통하는 방사대와 외측의 미세융모가 확 인되었다. 2011년 3월 수수미꾸리의 수정난을 주사전자현미경으로 관찰한 결과 난막의 표면에는 2~3 ${\mu}m$의 미세융모가 분포하였으며 외경이 크고 내경이 작아지는 깔대기 모양의 난문이 관찰되었다. 난문 주변의 미세융모는 평균 $5.26{\pm}1.22$ (6.79~3.39) ${\mu}m$로 난막 표면의 미세융모 보다 긴 것으로 확인되었다.

Keywords

References

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