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trnL-trnF 서열에 의한 한국 귤나무속과 두 근연 식물종의 계통분류학적 연구

Phylogenic Study of Genus Citrus and Two Relative Genera in Korea by trnL-trnF Sequence

  • 허만규 (동의대학교 분자생물학과) ;
  • 윤혜정 (부산대학교 생물교육과) ;
  • 최주수 (동의대학교 분자생물학과)
  • Huh, Man-Kyu (Department of Molecular Biology, Dongeui University) ;
  • Yoon, Hye-Jeong (Department of Science Education, Busan National University) ;
  • Choi, Joo-Soo (Department of Molecular Biology, Dongeui University)
  • 투고 : 2011.07.30
  • 심사 : 2011.09.09
  • 발행 : 2011.10.31

초록

귤나무속은 운향과(Rutaceae)에 속한다. 동남아시아, 미얀마, 중국 운남에서 기원된 것으로 알려져 있다. 이 속의 분류관계는 복잡한데 클론번식과 야생식물과 교잡이 빈번하다. 식물에서 속간 속내 계통관계 추론을 위해 널리 이용되고 있는 엽록체 trnL-trnF 부위가 있다. 이 귤나무속과 두 근연한 탱자나무속과 금감속에 속하는 7종간 계통 관계를 평가하였다. 많은 삽입이 발견되었고 속내 종간 변이는 결실/삽입에 의한 것으로 밝혀졌다. 이 속의 레몬과 당귤나무은 네 계통도(MP, ML, ME, and NJ)에서 모두 같은 분지군을 형성하여 가장 근연관계에 있었고 광귤나무(향귤나무)과 자매군을 형성하였다. 유자나무는 이들과 많은 서열 차이를 나타내었다. 외부 분지군은 낮은 지지도를 나타내었다.

Citrus is a common term and genus (Citrus) of flowering plants in the rue family, Rutaceae. Citrus is believed to have originated in the part of Southeast Asia bordered by Northeastern India, Myanmar (Burma) and the Yunnan province of China. The taxonomy and systematics of the genus are complex and the precise number of natural species is unclear, as many of the named species are clonally propagated hybrids, and there is genetic evidence that even some wild, true-breeding species are of a hybrid origin. One of the most popular sequences for phylogenetic inference at the generic and infrageneric levels in plants is the chloroplast trnL-trnF region. We evaluated the seven taxa with the trnL-trnF region to estimate phenotypic relationships within the genera Citrus, Poncirus, and Fortunella in Korea. Alignment of the DNA sequences required the addition of numerous gaps. Sequence variation within Citrus was mostly due to insertion/deletion. Within the genus Citrus, C. lomonia and C. sinensis were relatives and sistered with C. aurantium in the four phylogenetic analyses (MP, ML, ME, and NJ). However, some external nodes were poorly supported.

키워드

참고문헌

  1. Andrews, A. C. 1961. Acclimatization of citrus fruits in the Mediterranean region. Agricultural History 35, 35-46.
  2. Bonnard, G., F. Michel, J. H. Well, and A. Steinmetz. 1984. Nucleotide sequence of the $tRNA^{Leu}$ gene from Vicia faba chloroplasts: evidence for structural homologies of the $tRNA^{Leu}$ intron with intron from the autosplicable Tetrahymena ribosomal RNA precursor. Mol. Gen. Genet. 194, 330-336. https://doi.org/10.1007/BF00383536
  3. Cech, T. R. 1988. Conserved sequences and structures of group I introns: building an active site for RNA catalysis - a review. Gene 73, 259-271. https://doi.org/10.1016/0378-1119(88)90492-1
  4. Felsenstein, J. 1993. PHYLIP (Phylogeny Inference Package) version 3.5s, Distributed by the author. Department of Genetics, Univ. Washington, seattle.
  5. Freitas, de A. E., de Q. L. Paganucci, and M. A. Machado. 2003. What is Citrus? Taxonomic implications from a study of cp-DNA evolution in the tribe Citreae (Rutaceae subfamily Aurantioideae). Organisms Diversity & Evolution 3, 55-62. https://doi.org/10.1078/1439-6092-00058
  6. Gmitter, F. and X. Hu. 1990. The possible role of Yunnan, China, in the origin of contemporary Citrus species (Rutaceae). Economic Botany 44, 267-277. https://doi.org/10.1007/BF02860491
  7. Hirai, M. and I. Kajiura. 1987. Genetic analysis of leaf isozyme in Citrus. Japan J. Breed. 37, 377-388. https://doi.org/10.1270/jsbbs1951.37.377
  8. Kumar, S. and S. R. Gadagkar. 2001. Disparity Index: A simple statistic to measure and test the homogeneity of substitution patterns between molecular sequences. Genetics 158, 1321-1327.
  9. Lee, T. B. 2003. Coloured Flora of Korea. Hyangmoon-sa, Seoul. Korea.
  10. Lee, Y. N. 2007. New Flora of Korea. Kyo-Hak Publishing Co., Seoul. Korea.
  11. Michel, F. and B. Dujon. 1983. Conservation of RNA secondary structures in two intron families including mitochondrial-, chloroplast- and nuclear-encoded members. EMBO J. 2, 33-38.
  12. Nei, M. 1973. Analysis of gene diversity in subdivided populations. Proc. Natl. Acad. Sci. USA 70I, 3321-3323.
  13. Nei, M. and S. Kumar. 2000. Molecular Evolution and Phylogenetics. Oxford University Press, New York.
  14. Nicolosi, E., Z. N. Deng, A. Gentile, S. La Malfa, G. Continella, and E. Tribulato. 2000. Citrus phylogeny and genetic origin of important species as investigated by molecular markers. Theor. Appl. Genet. 100, 1155-1166. https://doi.org/10.1007/s001220051419
  15. Oh, J. B. and D. K. Moon. 2002. RAPD and phylogenic relationship of Citrus growing in Jeju Island. J. Subtropical Agri. Biotech. 18, 1-10.
  16. Saitou, N. and M. Nei. 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406-425.
  17. Scora, R. W. 1975. On the history and origin of citrus. Bulletin of the Torrey Botanical Club 102, 369-375. https://doi.org/10.2307/2484763
  18. Swofford, D. L. 2003. PAUP*. Phylogenetic Analysis Using Parsimony (*and other methods). Version 4. Sunderland, Sinauer Associates, Inc. MA.
  19. Taberlet, P., L. Gielly, G. Pautou, and J. Bouvet. 1991. Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Mol. Biol. 17, 1105-1109. https://doi.org/10.1007/BF00037152
  20. Tajima, F. 1989. Statistical methods to test for nucleotide mutation hypothesis by DNA polymorphism. Genetics 123, 585-595.
  21. Tamura, K., M. Nei, and S. Kumar. 2004. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc. Natl. Acad. Sci. (USA) 101, 11030-11035. https://doi.org/10.1073/pnas.0404206101
  22. Tamura, K., D. Peterson, N. Peterson, G. Stecher, M. Nei, and S. Kumar. 2011. MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Mol. Biol. Evol. (In Press).
  23. Woo, H. S. 1997. Studies on RAPD analysis hybrid plants of interspecific and intergeneric among Citrus and Poncirus triforiata (L.) Raf. MS. Thesis, Chonnam National University, Gwangju, Korea.
  24. Yun, S. H. 2001. Classification of genus Citrus and its related genera using RAPD. MS. Thesis, Jeju Natl. Univ., Jeju, Korea.