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DNA Barcode Examination of Bryozoa (Class: Gymnolaemata) in Korean Seawater

  • Lee, Hyun-Jung (Division of EcoScience, Ewha Womans University) ;
  • Kwan, Ye-Seul (Division of EcoScience, Ewha Womans University) ;
  • Kong, So-Ra (Division of EcoScience, Ewha Womans University) ;
  • Min, Bum-Sik (Department of Life Science, The University of Suwon) ;
  • Seo, Ji-Eun (Department of Rehabilitation, Woosuk University) ;
  • Won, Yong-Jin (Division of EcoScience, Ewha Womans University)
  • 투고 : 2011.05.16
  • 심사 : 2011.06.08
  • 발행 : 2011.07.31

초록

DNA barcoding of Bryozoa or "moss animals" has hardly advanced and lacks reference sequences for correct species identification. To date only a small number of cytochrome c oxidase subunit I (COI) sequences from 82 bryozoan species have been deposited in the National Center for Biotechnology Information (NCBI) GenBank and Barcode of Life Data Systems (BOLD). We here report COI data from 53 individual samples of 29 bryozoan species collected from Korean seawater. To our knowledge this is the single largest gathering of COI barcode data of bryozoans to date. The average genetic divergence was estimated as 23.3% among species of the same genus, 25% among genera of the same family, and 1.7% at intraspecific level with a few rare exceptions having a large difference, indicating a possibility of presence of cryptic species. Our data show that COI is a very appropriate marker for species identification of bryozoans, but does not provide enough phylogenetic information at higher taxonomic ranks. Greater effort involving larger taxon sampling for the barcode analyses is needed for bryozoan taxonomy.

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참고문헌

  1. Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R, 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3:294-299.
  2. Gomez A, Hughes RN, Wright PJ, Carvalho GR, Lunt DH, 2007. Mitochondrial DNA phylogeography and mating compatibility reveal marked genetic structuring and speciation in the NE Atlantic bryozoan Celleporella hyalina. Molecular Ecology, 16:2173-2188. https://doi.org/10.1111/j.1365-294X.2007.03308.x
  3. Kumar S, Tamura K, Nei M, 2004. MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Briefings in Bioinformatics, 5:150-163. https://doi.org/10.1093/bib/5.2.150
  4. Mackie JA, Keough MJ, Christidis L, 2006. Invasion patterns inferred from cytochrome oxidase I sequences in three bryozoans, Bugula neritina, Watersipora subtorquata, and Watersipora arcuata. Marine Biology, 149:285-295. https://doi.org/10.1007/s00227-005-0196-x
  5. Seo JE, 1999. Taxonomic review of Korean Watersipora (Bryozoa, Gymnolaemata, Cheilostomata). Korean Journal of Systematic Zoology, 15:221-229.
  6. Seo JE, 2005. Illustrated encyclopedia of fauna and flora of Korea. Vol. 40. Bryozoa. Ministry of Education and Human Resources Development, Seoul, pp. 1-596.
  7. Vrijenhoek RC, 2009. Cryptic species, phenotypic plasticity, and complex life histories: assessing deep-sea faunal diversity with molecular markers. Deep Sea Research Part II: Topical Studies in Oceanography, 56:1713-1723. https://doi.org/10.1016/j.dsr2.2009.05.016

피인용 문헌

  1. Molecular variability in the Celleporella hyalina (Bryozoa; Cheilostomata) species complex: evidence for cryptic speciation from complete mitochondrial genomes vol.39, pp.9, 2012, https://doi.org/10.1007/s11033-012-1714-9
  2. complex (Bryozoa, Cheilostomata) vol.43, pp.2, 2014, https://doi.org/10.1111/zsc.12042
  3. Resurrection of the genus Licornia for Scrupocellaria jolloisii (Bryozoa) and related species, with documentation of L. jolloisii as a non-indigenous species in the western Atlantic vol.93, pp.07, 2013, https://doi.org/10.1017/S0025315413000301
  4. sensu lato (Bryozoa, Buguloidea) vol.44, pp.3, 2015, https://doi.org/10.1111/zsc.12103