BMB Reports

생화학분자생물학회 (Korean Society for Biochemistry and Molecular Biology)
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Enhanced biological effects of Phe140Asn, a novel human granulocyte colony-stimulating factor mutant, on HL60 cells

  • Chung, Hee-Kyoung (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Sung-Woo (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Byun, Sung-June (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Ko, Eun-Mi (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Chung, Hak-Jae (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Woo, Jae-Seok (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Yoo, Jae-Gyu (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Lee, Hwi-Cheul (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Yang, Byoung-Chul (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Kwon, Moo-Sik (Department of Genetic Engineering, Sungkyunkwan University) ;
  • Park, Soo-Bong (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Park, Jin-Ki (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Kyung-Woon (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration)
  • 투고 : 2011.06.08
  • 심사 : 2011.06.23
  • 발행 : 2011.10.31
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초록

Granulocyte colony-stimulating factor (G-CSF) is a cytokine secreted by stromal cells and plays a role in the differentiation of bone marrow stem cells and proliferation of neutrophils. Therefore, G-CSF is widely used to reduce the risk of serious infection in immunocompromised patients; however, its use in such patients is limited because of its non-persistent biological activity. We created an N-linked glycosylated form of this cytokine, hG-CSF (Phe140Asn), to assess its biological activity in the promyelocyte cell line HL60. Enhanced biological effects were identified by analyzing the JAK2/STAT3/survivin pathway in HL60 cells. In addition, mutant hG-CSF (Phe140Asn) was observed to have enhanced chemoattractant effects and improved differentiation efficiency in HL60 cells. These results suggest that the addition of N-linked glycosylation was successful in improving the biological activity of hG-CSF. Furthermore, the mutated product appears to be a feasible therapy for patients with neutropenia.

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참고문헌

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피인용 문헌

  1. Differences in gene expression and alterations in cell cycle of acute myeloid leukemia cell lines after treatment with JAK inhibitors vol.765, 2015, https://doi.org/10.1016/j.ejphar.2015.08.037
  2. Antiapoptotic effects of Phe140Asn, a novel human granulocyte colony-stimulating factor mutant in H9c2 rat cardiomyocytes vol.45, pp.12, 2012, https://doi.org/10.5483/BMBRep.2012.45.12.095
  3. Soluble Prokaryotic Overexpression and Purification of Bioactive Human Granulocyte Colony-Stimulating Factor by Maltose Binding Protein and Protein Disulfide Isomerase vol.9, pp.3, 2014, https://doi.org/10.1371/journal.pone.0089906