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ITS 서열에 의한 한국 왕대속 식물종의 계통분류학적 연구

Phylogenic Study of Genus Phyllostachys (Phyllostachys) in Korea by Internal Transcribed Spacer Sequence (ITS)

  • 이송진 (부산대학교 생물교육과) ;
  • 허만규 (동의대학교 분자생물학과) ;
  • 허홍욱 (부산대학교 생물교육과) ;
  • 이병룡 (서원대학교 과학교육과)
  • Lee, Song-Jin (Department of Science Education, Busan National University) ;
  • Huh, Man-Kyu (Department of Molecular Biology, Dongeui University) ;
  • Huh, Hong-Wook (Department of Science Education, Busan National University) ;
  • Lee, Byeong-Ryong (Department of Science Education (Biology), Seowon University)
  • 투고 : 2011.07.11
  • 심사 : 2011.09.02
  • 발행 : 2011.09.30

초록

왕대속은 벼과 또는 화본과에 속하는 다년생 목본성 초본이며 아시아의 인도와 중국에 많은 종이 주로 분포한다. 식물에서 속간 속내 계통관계 추론을 위해 널리 이용되고 있는 ITS 부위가 있다. 이 속에 속하는 우리나라 자생 식물 왕대 속내 네 식물종 간 계통 관계를 평가하기 위해 핵 게놈의 ITS 부위로 평가하였다. DNA 서열 배당은 많은 갭(gaps)을 가지고 있었다. 이 속 내 서열 변이는 일부 삽입과 결실이 발견되었고 속내 종간 변이는 자연도태에 의한 것으로 밝혀졌다. 이 속의 오죽과 분죽은 세 계통도(MP, ML, and NJ)에서 모두 같은 분지군을 형성하여 가장 근연관계에 있었다. 기존의 형태학적 특성과 단순 반복 서열(ISSR)의 결과와 유사한 계통 관계를 나타내어 왕대속에서 ITS의 서열이 이들 분류군에 매우 유익한 정보를 제공하고 있음을 시사하였다.

Phyllostachys consists of high and fast growing trees and is a genus in the family Gramineae. The genus has many species in Asia, with main distribution being in India and China. One of the most popular sequences for phylogenetic inference at the generic and infrageneric levels in plants is the internal transcribed spacer (ITS) region of the 18S-5.8S-26S nuclear ribosomal cistron. We evaluated four taxa with the ITS region to estimate phenotypic relationships within the genus Phyllostachys in Korea. Alignment of the DNA sequences required the addition of numerous gaps. Sequence variation within the Phyllostachys was mostly due to natural selection, although several indels and inserts were found. Within the genus Phyllostachys, P. nigra and P. nigra var. henonis were the relatives in the three phylogenetic analyses (MP, ML, and NJ). However, some external nodes were poorly supported. Morphological traits and simple repeats (ISSR) represented the result of a relationship similar to the that of ITS sequences in the genus Phyllostachys. This suggests that ITS sequences are very informative for identification of these taxa.

키워드

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