생명과학회지 (Journal of Life Science)

  • 제21권9호
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  • Pages.1266-1273
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  • 2011
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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항산화 효능을 가진 사포닌이 사람섬유아세포에서 기질 금속 단백질 분해효소에 미치는 영향

Effect of Saponin with Antioxidant Activity on Matrix Metalloproteinase in Human Dermal Fibroblasts

  • 투고 : 2011.07.04
  • 심사 : 2011.08.09
  • 발행 : 2011.09.30
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초록

사포닌은 동양의 전통적인 약재로 널리 알려진 인삼의 주요한 성분이다. 사포닌의 다양한 생물학적 효능이 밝혀져 있으나, 피부재생과 관련된 효능은 현재까지도 명백하지 않다. 본 연구에서는 세포외 시스템에서 사포닌의 항산화 효과 뿐만 아니라 사람 진피 섬유아세포에서 기질금속단백질 분해효소(MMP)에 대한 효능이 조사되었다. 먼저 MTT assay를 이용한 세포생존력에 대한 사포닌의 효능을 조사한 결과, 10 ${\mu}g$/ml 이하의 농도에서 사포닌은 세포생존력을 증가시켰으나 25 ${\mu}g$/ml 이상의 농도에서는 세포독성을 나타내었다. 항산화에 대한 사포닌 효능을 조사한 결과, 1 ${\mu}g$/ml 이상의 농도에서 사포닌은 환원력 뿐만 $H_2O_2$에 대한 억제 효능을 보여주었다. 특히, DNA 산화에 대한 보호 효과도 나타내었다. 더욱이 gelatin 및 casein zymography 시험결과, 사포닌은 MMP-2를 활성화 시키고 MMP-1의 활성을 증가시키는 것으로 보아, 항노화 및 피부재생 약효제로 잠재적인 가능성이 기대된다.

Saponin is a main component of ginseng widely known as an oriental traditional medicinal ingredient. A variety of biological effects of saponin has been reported, but its action related to skin regeneration has remained unclear so far. In this study, the effect of saponin on matrix metalloproteinase as well as its antioxidant effect in cell free system was examined in human dermal fibroblasts. First of all, as a result of investigating the effect of saponin on cell viability using MTT assay, it was shown to increase cell viability below 10 ${\mu}g$/ml, but it also showed cytotoxicity above 25 ${\mu}g$/ml. The antioxidant effect of saponin was exerted by inhibition of $H_2O_2$ in addition to reducing power above 1 ${\mu}g$/ml. In particular, saponin showed a protective effect on DNA oxidation. Furthermore, it was observed that saponin activates MMP-2 and increases MMP-1 activity in gelatin and casein zymography analyses, respectively, indicating that saponin could have potential a therapeutic agent for anti-aging and skin regeneration.

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