한국어병학회지 (Journal of fish pathology)

  • 제24권1호
  • /
  • Pages.39-45
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  • 2011
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  • 1226-0819(pISSN)
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  • 2233-5412(eISSN)
한국어병학회 (The Korean Society of Fish Pathology)
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수온에 따른 넙치의 특이 항체 반응

Specific antibody response of olive flounder Paralichthys olivaceus by water temperature

  • 김위식 (전남대학교 수산과학연구소) ;
  • 장민석 (전남대학교 수산생명의학과) ;
  • 정성주 (전남대학교 수산생명의학과) ;
  • 김석렬 (국립수산과학원 병리연구과) ;
  • 박명애 (국립수산과학원 병리연구과) ;
  • 이정호 (국립수산과학원 육종연구센터) ;
  • 명정인 (국립수산과학원 육종연구센터) ;
  • 오명주 (전남대학교 수산생명의학과)
  • Kim, Wi-Sik (The Fisheries Science Institute, Chonnam National University) ;
  • Jang, Min-Seok (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University) ;
  • Jung, Sung-Ju (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University) ;
  • Kim, Seok-Ryel (Pathology Division, National Fisheries Research and Development Institute) ;
  • Park, Myoung-Ae (Pathology Division, National Fisheries Research and Development Institute) ;
  • Lee, Jeong-Ho (Genetics & Breeding Research Center, National Fisheries Research & Development Institute) ;
  • Myeong, Jeong-In (Genetics & Breeding Research Center, National Fisheries Research & Development Institute) ;
  • Oh, Myung-Joo (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University)
  • 투고 : 2010.12.09
  • 심사 : 2011.03.16
  • 발행 : 2011.04.30
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초록

본 연구에서는 넙치를 대상으로 항체 검출 ELISA법을 적용하기 위한 기초연구로서 넙치에 BSA를 면역시킨 후 사육 수온에 따른 특이 항체 반응을 항체 검출 ELISA법을 사용하여 조사하였다. 수온 $15^{\circ}C$에서는 면역 후 14일째부터 BSA에 대한 특이 항체가가 형성되는 개체가 확인되었고 (OD값: 0.69), 28~42일에 가장 높은 항체가 (OD값: 1.94~3.04)가 관찰되었으며, 면역 84일째는 0.03~1.28의 OD값이 관찰되었다. 수온 $12{\sim}13^{\circ}C$에서는 면역 후 28일째부터 항체가 관찰되었고 (OD값: 0.14~0.25), 3개체에서 56~70일에 가장 높은 항체가 (OD값: 1.88~2.68)가 확인되었으며, 면역 84일째에는 0.49~2.35의 OD값이 관찰되었다. 2개체의 경우는 면역 84일까지 0.8 이하의 OD값을 나타내었다. 수온 $10^{\circ}C$에서는 면역 후 56일째부터 항체가가 확인되었고 (OD값: 0.11~0.83), 2개체에서 면역 70일째 가장 높은 항체가가 (OD값: 1.37~1.53)가 확인되었으며, 면역 84일째에는 1.00~1.11의 OD값이 관찰되었다. 이에 반해 3개체에서는 항체가가 천천히 상승하여 면역 84일째 0.12~0.68의 OD값을 나타내었다. 이상의 결과로 넙치의 특이 항체 반응은 개체별로 차이를 보이나 수온이 높을수록 항체 형성이 빠르고 항체가도 높게 나타나며, 지속기간이 길게 나타나는 것이 확인되었다.

The specific antibody response of olive flounder, Paralichthys olivaceus to different water temperature were tested by enzyme-linked immunosorbent assay (ELISA). In the rearing temperature of $15^{\circ}C$, first anti-bovine serum albumin (BSA) antibody titer was appeared after 14 days of immunization, whereas 24~48 days post-immunization (PI) resulted maximum antibody titer in all 5 experimental fish with optical density (OD) values 1.94~3.04. At the end of the experiment (84 days), 0.03~1.28 OD values were observed. In the rearing temperature of $12{\sim}13^{\circ}C$, first antibody titer was found 28 days PI in 2 out of 5 fish. Three fish shown high OD titer (1.88~2.68) between 56 and 70 days and OD values of 0.49 to 2.35 were observed at 84 days. However, the anti-BSA antibodies of two fish showed less than 0.8 OD values until 84 days. In the rearing temperature of $10^{\circ}C$, specific antibody appeared at 56 days, maximum antibody titer was observed at 70 days in 2 out of 5 fish (OD values: 1.37~1.53) and 1.00 to 1.11 OD values were observed at 84 days. Rest 3 fish showed OD values of 0.12 to 0.68 much below to that of other 2 fish, throughout the experimental period. In conclusion, specific antibody response of olive flounder at high temperature was much faster, higher and longer than that at lower temperature.

키워드

참고문헌

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피인용 문헌

  1. 항원의 투여방법 및 사육환경 변화에 따른 넙치의 특이항체 반응 vol.24, pp.2, 2011, https://doi.org/10.7847/jfp.2011.24.2.153
  2. 넙치(Paralichthys olivaceus)의 immunoglobulin M에 대한 단클론 항체 생산 vol.50, pp.2, 2011, https://doi.org/10.5657/kfas.2017.0169
  3. Enzyme-linked immunosorbent assay를 이용한 바이러스성 출혈성 패혈증 바이러스 감염 넙치(Paralichthys olivaceus)의 특이 항체반응 검사 vol.50, pp.5, 2011, https://doi.org/10.5657/kfas.2017.0547