Journal of Oriental Neuropsychiatry (동의신경정신과학회지)

The Korean Society of Oriental Neuropsychiatry (대한한방신경정신과학회)
권호 표지
DOI QR코드

DOI QR Code

The Effects of DoDamTanghapChongMungTang(Daotantanghecongmingtang) on LPS induced-Microglia and Memory Deficit Mice Model

도담탕합총명탕(導痰湯合聰明湯)이 LPS로 처리된 microglia 및 기억력 감퇴 생쥐 모델에 미치는 영향

  • Park, Dae-Myung (Dept. of Neuropsychiatry, College of Oriental Medicine, Dae-Jeon University) ;
  • Lee, Sang-Ryong (Dept. of Neuropsychiatry, College of Oriental Medicine, Dae-Jeon University) ;
  • Jung, In-Chul (Dept. of Neuropsychiatry, College of Oriental Medicine, Dae-Jeon University)
  • 박대명 (대전대학교 한의과대학 신경정신과학교실) ;
  • 이상룡 (대전대학교 한의과대학 신경정신과학교실) ;
  • 정인철 (대전대학교 한의과대학 신경정신과학교실)
  • Received : 2011.05.09
  • Accepted : 2011.06.13
  • Published : 2011.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : This experiment was designed to investigate the efficacy of DDTCMT hot water extract & ultra-fine powder on Alzheimer's Disease Model. Methods : The effects of the DDTCMT hot water extract on expression of IL-$1{\beta}$, IL-6, TNF-${\alpha}$, COX-2, NOS-II, IL-10, IL-1 receptor antagonist mRNA and production of IL-$1{\beta}$, IL-6, TNF-${\alpha}$ in BV2 microglial cell line treated by lipopolysacchaide(LPS) were investigated. Expression of NO, ROS in BV2 microglial cell line treated by LPS and AChE activity in PC-12 cell treated by NGF were investigated. anti-AChE was observed through Western blot analysis. The effects of the DDTCMT hot water extract & ultra-fine powder on the behavior of the memory deficit mice induced by scopolamine were investigated. Results : 1. The DDTCMT hot water extract significantly decreased the production of mIL-6, mNOS-II, mTNF-${\alpha}$, and increased the production of mIL-10, mIL-1 receptor antagonist. 2. The DDTCMT hot water extract significantly suppressed the production of IL-$1{\beta}$, IL-6, TNF-${\alpha}$ in BV2 microglial cell line treated by LPS. 3. The DDTCMT hot water extract significantly suppressed the NO and ROS production in BV2 microglial cell line treated by LPS. 4. The DDTCMT hot water extract groups showed inhibition of AChE activity in NGF treated PC-12 cell line. 5. The DDTCMT hot water extract suppressed anti-AChE expression in NGF treated PC-12 cell line was observed by Western blot analysis. 6. The DDTCMT hot water extract & ultra-fine powder groups showed significantly inhibitory effect on the scopolamine -induced impairment of memory in the experiment of Morris water maze. Conclusions : These results suggest that the DDTCMT hot water extract & ultra-fine powder may be effective for the prevention and treatment of Alzheimer's disease.

Keywords

References

  1. 전국한의과대학 신경정신과 교과서편찬위원회. 한의신경정신과학. 경기:집문당. 2007:324-33.
  2. 민성길. 최신정신의학. 서울:일조각. 2004:189-200, 611-2.
  3. 이광우. 임상신경학. 서울:고려의학. 2002:203-8.
  4. Cummings JL. Frontal-subcortical circuits and human behavior. Arch Neurol. 1993;50:873-80. https://doi.org/10.1001/archneur.1993.00540080076020
  5. Oide, T, Kinoshita, T, Arima, K. Regression stage senile plaques in the natural course of alzheimer's disease. Neuropathology appl neurobiology. 2006;32(5):539-56. https://doi.org/10.1111/j.1365-2990.2006.00767.x
  6. David, S. Treatment guidelines for alzheimer's disease - redefinding perceptions in primary care. Primary care companion J clinic psychiatry. 2007;9(2):113-21. https://doi.org/10.4088/PCC.v09n0205
  7. McGrath LT. Increased oxidative stress in alzheimer's disease as assessed with 4-hydroxynonenal but not malondialdehyde. Q J medicin. 2001;94:485-90. https://doi.org/10.1093/qjmed/94.9.485
  8. Ramazan O, Namik D. Malondialdehyde, superoxide dismutase, melatonin, iron, copper, and zinc blood concentrations in patients with Alzheimer disease: cross-sectional study. Clinical sciences. 2002;43(1):28-32.
  9. 孫思邈. 備急千金要方. 서울:행림출판사. 1982:129-35, 534, 545, 550.
  10. 王肯堂. 證治準繩. 서울:翰成社. 1982:306-7.
  11. 洪元植. 精校黃帝內經素問. 서울:洋醫學硏究院. 1985:37, 124, 196, 217-8, 229.
  12. 張介賓. 張氏景岳全書. 서울:翰成社. 1978:610-1.
  13. 陳輝. 實用中醫腦病學. 北京:學苑出版社. 1993:793-5.
  14. 許浚. 東醫寶鑑. 서울:동의보감출판사. 2005:98-100.
  15. 李尙仁. 漢藥臨床應用. 서울:成輔社. 1990:151-3, 308-13, 419-20, 426-8.
  16. 박지운, 정인철, 이상룡. 聰明湯과 木槿皮聰明湯이 CT105와 βA로 유도된 Alzheimer's Disease病態 모델에 미치는 영향. 동의신경정신과학회지. 2006;17(1):37-5.
  17. 하수영, 정인철, 이상룡. 聰明湯과 山査聰明湯이 Alzheimer's Disease 病態 모델에 미치는 影響. 동의신경정신과학회지. 2006;17(1):59-78.
  18. 최강욱, 이상룡, 정인철. 聰明湯과 木槿皮聰明湯 열수추출물, 초미세분말제형이 microglia 및 기억력 감퇴 병태모델에 미치는 영향. 동의생리병리학회지. 2006;20(5):1200-10.
  19. 徐承浩, 鄭仁哲, 李相龍. 聰明湯和山査聰明湯 熱水提取物, 超微細粉末劑型對microglia及記 憶力減退病態模型的影響. 第5會天津國際中醫藥學術硏討會議論文集. 2006;325-7.
  20. 임정화, 정인철, 이상룡. 총명탕과 파극천총명탕 열수추출물, 초미세분말제형이 microglia 및 기억력 감퇴 병태모델에 미치는 영향. 동의생리병리학회지. 2006;20(4):997-1008.
  21. 오영진, 김보경. 聰明湯과 香附子聰明湯의 추출물, 나노분말 제형을 이용한 치매에 관한 연구. 동의신경정신과학회지. 2006;17(1):79-105.
  22. 嚴用和. 中訂嚴氏濟生方. 北京:人民衛生出版社. 1987:79.
  23. 전국 한의과대학 심계내과학교실. 心系內科學. 서원당. 1999:73, 77, 277-9, 420-2.
  24. 李仲梓. 醫宗必讀. 台北:文光圖書公司. 1976:217.
  25. 吳謙. 醫宗金鑑. 北京:人民衛生出版社. 1982:1036-7.
  26. 김연두, 문병순, 박영순, 김세길. 導痰湯이 家兎의 高脂血症 및 血栓症에 미치는 영향. 한국전통의학지. 1994:85-128.
  27. 조현경, 임승민, 안정조, 최영, 김용진, 유호룡, 박양춘, 설인찬, 황치원. 導痰湯이 뇌손상 및 고혈압에 미치는 영향. 대한한방내과학회지. 2001;22(4):503-12.
  28. 최현정, 방나영, 송보완, 김남재, 류봉하. 한약제형 선호도에 관한 설문조사. 경희의학. 2004;20(1):356-67.
  29. 김현수, 이상룡, 정인철. 세심탕 열수추출물, 초미세분말제형이 Alzheimer's Disease 병태모델에 미치는 영향. 동의생리병리학회지. 2007;21(3):688-99.
  30. 최강욱, 정인철. 동의신경정신과학회지. 천왕보심단 열수추출물, 초미세분말제형이 Alzheimer's Disease 병태 모델에 미치는 영향. 2008;19(2):77-93.
  31. 서승호, 정인철, 이상룡. 가미보양환오탕 열수추출물, 초미세분말제형이 Alzheimer's Disease 병태 모델에 미치는 영향. 동의신경정신과학회지. 2008;29(3):69-84.
  32. 이승희, 이상룡, 정인철. 귀비총명탕 열수추출물과 초미세분말제형이 Alzheimer's Disease 병태 모델에 미치는 영향. 동의생리병리학회지. 2007;21(4):921-33.
  33. 임정화, 이상룡. 팔미합총명탕 열수추출물, 초미세분말제형이 Alzheimer's Disease 병태 모델에 미치는 영향. 동의신경정신과학회지. 2008;21(4):95-110.
  34. 민경직, 이상룡, 정인철. 성심지황탕 열수추출물과 초미세분말제형이 Alzheimer's Disease 병태 모델에 미치는 영향. 동의생리병리학회지. 2008;22(5):1178-91.
  35. 최보윤, 이상룡, 정인철. 각병연수장합증손백출산 열수추출물, 초미세분말제형이 Alzheimer's Disease 병태 모델에 미치는 영향. 대한한의학회지. 2007;28(2):137-54.
  36. Skehan P, Storeng R, Scudiero D, Monk A, McMahon J, Visca D, Warren JT, Kennedy S, Boyd MR. New colorimetric cytotoxicity assay for anticancer drug screening. Journal of the National Cancer Institute. 1990;82(13):1107-12. https://doi.org/10.1093/jnci/82.13.1107
  37. 이부영 역. ICD-10 정신 및 행동장애 분류. 서울:일조각. 1994:63-6.
  38. 서무규. 성인병.노인병학. 서울:고려의학. 1986:225-67.
  39. 한일우. Alzheimer병의 증상. 계명의대논문집. 1997;16(3):339-47.
  40. Cacquevel M, Lebeurrier N, Cheenne S, Vivien D. Cytokines in neuroinflammation and Alzheimer's disease. Current Drug Targets. 2004;5(6):529-34. https://doi.org/10.2174/1389450043345308
  41. Kuhl DE, Koeppe RA, Minoshima S, Snyder SE, Ficaro EP, Foster NL, Frey KA, Kilbourn MR. In vivo mapping of cerebral acetylcholinesterase activity in aging and Alzheimer's disease. Neurology. 1999;52(4):691-9. https://doi.org/10.1212/WNL.52.4.691
  42. Mhatre M, Floyd RA, Hensley K. Oxidative stress and neuroinflammation in Alzheimer's disease and amyotrophic lateral sclerosis: common links and potential therapeutic targets. Journal of Alzheimers disease. 2004;6(2):147-57.
  43. 안준익, 이용성. Beta-amyloid의 분자생물학. 한양의대학술지. 2001;21(1):11-6.
  44. Kasa P, Papp H, Torok I. Donepezil dose-dependently inhibits acetylcholinesterase activity in various areas and in the presynaptic cholinergic and the postsynaptic cholinoceptive enzyme-positive structures in the human and rat brain. Neuroscience. 2000;101(1):89-100. https://doi.org/10.1016/S0306-4522(00)00335-3
  45. Spassov A, Getova DP, Dimitrova DS. P4.025 Study of ancholinesterase drugs galanthamine and donepezil on scopolamine-induced amnesia in rats. European Neuropsychopharmacology. 2004;14:333.
  46. Summers WK. Tacrine (THA, cognex). Journal of alzheimer. 2000;2(2):85-93.
  47. Yen GC , Lai HH Nitric oxide-scavenging and antioxidant effects of Uraria crinita root. Food Chemistry. 2001;74:471-8. https://doi.org/10.1016/S0308-8146(01)00165-0
  48. Mates, JM. Antioxidant enzymes and human disease. Clinical Biochemistry. 1999;32:595. https://doi.org/10.1016/S0009-9120(99)00075-2
  49. 陳士鐸. 石室秘錄. 北京:中國中醫藥出版社. 1991:125.
  50. 錢鏡湖. 辨證奇問全書. 台北:甘地出版社. 1990:222-5, 233-5.
  51. 許沛虎. 中醫腦病學. 北京:中醫醫藥科技出版社. 1998:464-5.
  52. 薜己. 校注婦人良方 (卷三). 서울:金泳出版社. 1962:1-13.
  53. 全國韓醫科大學本草學敎室. 本草學. 서울:永林社. 1991:136-7, 302-4, 350-1, 400-1, 419-20, 448-51, 466-7, 509-10, 523-4, 531-3, 540-1.
  54. Moschwitzer J, Muller RH. New method for the effective production of ultrafine drug nanocrystals. J Nanosci Nanotechnol. 2006;6(9,10):3145-53. https://doi.org/10.1166/jnn.2006.480
  55. Qiu RL, Li L, Li X, Zhang HX, Chen JW. Effect of ultra-fine powder technique on dissolution rates of andrographolide and dehydroandrographolide in Andrographis paniculata. Journal of chinese intergrative medicine. 2004;2(6):456-8. https://doi.org/10.3736/jcim20040618
  56. Rogers J, Mastroeni D, Leonard B, Joyce J, Grover A. Neuroinflammation in Alzheimer's disease and Parkinson's disease: are microglia pathogenic in either disorder?. Int Rev Neurobiol. 2007;82:235-46. https://doi.org/10.1016/S0074-7742(07)82012-5
  57. Nick JA, Avdi NJ, Gerwins P, Johnson GL, Worthen GS. Activation of p38 mitogen-activated protein kinase in human neutrophils by lipopolysaccharide. J Immunol. 1996;156(12):4867-75.
  58. Woo MS. Selective modulation of lipopolysaccharide-stimulated cytokine expression and mitogen-activated protein kinase pathways by dibutyryl-cAMP in BV2 microglial cells. Molecular Brain Research. 2003;113(1,2):86-96. https://doi.org/10.1016/S0169-328X(03)00095-0
  59. Kim YS. Microglia, major player in the brain inflammation: their roles in the pathogenesis of Parkinson's disease. Experimental and molecular medicine. 2006;38(4):333-47. https://doi.org/10.1038/emm.2006.40
  60. Pasinatti GM, Alsen PS. Cyclooxygenase-2 expression is increased in frontal cortex of Alzheimer's disease brain. Neuroscience. 1998;87(2):319-24. https://doi.org/10.1016/S0306-4522(98)00218-8
  61. Marcheselli VL, Bazan NG. Sustained induction of prostaglandin endoperoxide-2 by seizure in hippocampus. The Journal of biological chemistry. 1997;271:24794-9.
  62. Fernandez-Vizarra P. Expression of nitric oxide system in clinically evaluated cases of Alzheimer's disease. Neurobiology of Disease. 2004;15(2):287-305. https://doi.org/10.1016/j.nbd.2003.10.010
  63. Culpan D, Prince JA, Matthews S, Palmer L, Hughes A, Love S, Kehoe PG, Wilcock GK. Neither sequence variation in the IL-10 gene promoter nor presence of IL-10 protein in the cerebral cortex is associated with Alzheimer's disease. Neurosci Lett. 2006;13;408(2):141-5. https://doi.org/10.1016/j.neulet.2006.08.068
  64. Rothwell NJ, Luheshi GN. Interleukin 1 in the brain: biology, pathology and therapeutic target. Trends Neurosci. 2000;23:618-25. https://doi.org/10.1016/S0166-2236(00)01661-1
  65. Yamazaki M, Matsuoka N, Kuratani K, Ohkubo Y, Yamaguchi I. FR121196, a potential antidementia drug, ameliorates the impaired memory of rat in the Morris water maze. J Phamacol Exp Ther. 1995;272(1):256-63.

Cited by

  1. The Effects of Oriental Herbal Tea on the Brain Function Quotient of Elders at Health Facility vol.19, pp.2, 2013, https://doi.org/10.14370/jewnr.2013.19.2.128
  2. The Effects of Oriental Herbal Tea on the Physiological Function and Behavioral Change of Elders at Geriatric Facility vol.15, pp.3, 2014, https://doi.org/10.5762/KAIS.2014.15.3.1448