Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Neuroprotective Compounds Isolated from the Methanolic Extract of Lonicera japonica

  • Weon, Jin-Bae (Department of Biomaterials Engineering, School of Bioscience and Biotechnology, Kangwon National University) ;
  • Yang, Hye-Jin (Department of Biomaterials Engineering, School of Bioscience and Biotechnology, Kangwon National University) ;
  • Lee, Bo-Hyoung (Department of Biomaterials Engineering, School of Bioscience and Biotechnology, Kangwon National University) ;
  • Yun, Bo-Ra (Department of Biomaterials Engineering, School of Bioscience and Biotechnology, Kangwon National University) ;
  • Choong, Je-Ma (Department of Biomaterials Engineering, School of Bioscience and Biotechnology, Kangwon National University)
  • Received : 2011.04.18
  • Accepted : 2011.06.24
  • Published : 2011.09.30
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Abstract

A $CH_3Cl$ extract from the flower of Lonicera japonica (Lauraceae) significantly protected primary cultures of rat cortical cells injured by the excitotoxic amino acid, L-glutamate. Loganin (1), secoxyloganin (2), caffeic acid (3) rutin (4), hyperoside (5), quercetin-3-O-glucoside (6), lonicerin (7), kaempferol-3-O-rutinoside (8), luteolin-7-O-b-D-glucopyranoside (9), quercetin (10) and luteolin (11) were isolated by bioactivity-guided fractionation from the $CH_3Cl$ fraction and further separated using chromatographic techniques. Caffeic acid, lonicerin, kaempferol-3-O-rutinoside, quercetin and luteolin had significant neuroprotective activities against glutamate-induced neurotoxicity in primary cultures of rat cortical cells at concentrations ranging from $0.1{\mu}M$ to $10.0\;{\mu}M$.

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References

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