Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Different Level of Plasma Free Hemoglobin between Qi-deficiency and Fire Heat among Korean Stoke Subjects

한국인 중풍환자의 기허군 화열군의 plasma free hemoglobin의 비교

  • Lim, Ji-Hye (Brain Disease Research Center, Korea Institute of Oriental Medicine) ;
  • Ko, Mi-Mi (Brain Disease Research Center, Korea Institute of Oriental Medicine) ;
  • Lee, Jung-Sup (National Rehabilitation Center) ;
  • Lee, Myeong-Soo (Brain Disease Research Center, Korea Institute of Oriental Medicine) ;
  • Cha, Min-Ho (Brain Disease Research Center, Korea Institute of Oriental Medicine)
  • 임지혜 (한국한의학연구원 표준화연구본부 뇌질환연구센터) ;
  • 고미미 (한국한의학연구원 표준화연구본부 뇌질환연구센터) ;
  • 이정섭 (국립재활원) ;
  • 이명수 (한국한의학연구원 표준화연구본부 뇌질환연구센터) ;
  • 차민호 (한국한의학연구원 표준화연구본부 뇌질환연구센터)
  • Received : 2011.05.23
  • Accepted : 2011.07.22
  • Published : 2011.08.25
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Abstract

The purpose of this study was to fine proteins, which have significantly different level in plasma between Qi-deficiency and Fire-heat group of Korean Oriental Stroke pattern identification (PI) among Korean stroke patients. Eighteen stroke patients with Qi-deficiency and forty nine patients with Fire-heat, which had critical syndrome of each PI, were participated in this study. Plasma protein pattern were analyzed by SELDI-TOF MS using Q10 strong anion exchange chip and Mass spectral data (m/z) statistically determined. The expression level of proteins, which were different between Qi-deficiency and Fire-heat in the results by SELDI-TOF MS, were confirmed by western blot. As a result of analyzing plasma protein by SELDI-TOF MS, six protein peaks were significantly higher in Fire-heat group than Qi-deficiency group. Two peaks among of them, M15003 and M15745, were respectively identified as hemoglobin alpha and beta in previous study. Expression level of plasma free hemoglobin of Fire-heat group was also confirmed higher in Fire-heat group than in Qi-deficiency group. These findings suggest that plasma free hemoglobin is a candidate for discriminating Qi-deficiency and Fire-heat group according to pattern identification (PI) of stroke.

Keywords

References

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