The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
권호 표지
DOI QR코드

DOI QR Code

RT-PCR Detection of Five Quarantine Plant RNA Viruses Belonging to Potyand Tospoviruses

  • Lee, Jong-Seung (Department of Agricultural Biotechnology and Plant Breeding and Genomics Institute, College of Agriculture and Life Sciences, Seoul National University) ;
  • Cho, Won-Kyong (Department of Agricultural Biotechnology and Plant Breeding and Genomics Institute, College of Agriculture and Life Sciences, Seoul National University) ;
  • Choi, Hong-Soo (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Kook-Hyung (Department of Agricultural Biotechnology and Plant Breeding and Genomics Institute, College of Agriculture and Life Sciences, Seoul National University)
  • 투고 : 2011.07.13
  • 심사 : 2011.08.03
  • 발행 : 2011.09.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

In order to detect quarantine plant viruses, we developed reverse transcription-polymerase chain reaction (RT-PCR) primer pairs for five single-stranded (ss) plant RNA viruses that are not currently reported in Korea but could be potential harmful plant viral pathogens. Three viruses such as Chilli veinal mottle virus (ChiVMV), Colombian datura virus (CDV), and Tobacco etch virus (TEV) belong to the genus Potyvirus while Chrysanthemum stem necrosis virus (CSNV) and Iris yellow spot virus (IYSV) are members of the genus Tospovirus. To design RT-PCR primers, we used reported gene sequences corresponding to the capsid protein and polyprotein for ChiVMV, CDV, and TEV while using nucleocapsid protein regions for CSNV and IYSV. At least two different primer pairs were designed for each virus. Fifteen out of 16 primer pairs were successfully applied in detection of individual quarantine virus with high specificity and efficiency. Taken together, this study provides a rapid and useful protocol for detection of five quarantine viruses.

키워드

참고문헌

  1. Bezerra, I. C., Resende, R. O. de., Pozzer, L., Nagata, T., Kormelink, R. and Avila, A. C. De. 1999. Increase of tospoviral diversity in Brazil with the identification of two new tospovirus species, one from chrysanthemum and one from zucchini. Phytopathology 89:823-830. https://doi.org/10.1094/PHYTO.1999.89.9.823
  2. Carrasco, P., Daros, J. A., Agudelo-Romero, P. and Elena, S. F. 2007. A real-time RT-PCR assay for quantifying the fitness of tobacco etch virus in competition experiments. J. Virol. Methods 139:181-188. https://doi.org/10.1016/j.jviromet.2006.09.020
  3. Choi, M.-Y., Lee, S.-G., Kim, K.-H., Park, H.-H. and Kim, J. J. 2009. Recent outbreak of insect pests and climate change in Korea. International symposium on climate change and insect pest 3-304.
  4. Diaz-Montano, J., Fuchs, M., Nault, B. A., Fail, J. and Shelton, A. M. 2011. Onion thrips (Thysanoptera: Thripidae): A global pest of increasing concern in onion. J. Econ. Entomol. 104:1-13. https://doi.org/10.1603/EC10269
  5. Elena, S. F., Bedhomme, S., Carrasco, P., Cuevas, J. M., de la Iglesia, F., Lafforque, G., Lalic, J., Prosper, A., Tromas, N. and Zwart, M. P. 2011. The evolutionary genetics of emergin plant RNA viruses. Mol. Plant-Microbe. Interact. 24:287-293. https://doi.org/10.1094/MPMI-09-10-0214
  6. Garrett, K. A., Dendy, S. P., Frank, E. E., Rouse, M. N. and Travers, S. E. 2006. Climate change effects on plant disease: Genomes to ecosystems. Annu. Rev. Phytopathol. 44:489-509. https://doi.org/10.1146/annurev.phyto.44.070505.143420
  7. Harper, S. J., Delmiglio, C., Ward, L. I. and Clover, G. R. 2011. Detection of tomato black ring virus by real-time one-step RTPCR. J. Virol. Methods 171:190-194. https://doi.org/10.1016/j.jviromet.2010.10.023
  8. Hwang, J., Li, J., Liu, W. Y., An, S. J., Cho, H., Her, N. H., Yeam, I., Kim, D. and Kang, B. C. 2009. Double mutations in eIF4E and eIFiso4E confer recessive resistance to Chilli veinal mottle virus in pepper. Mol. Cells 27:329-336. https://doi.org/10.1007/s10059-009-0042-y
  9. Lee, J.-S., Cho, W. K., Lee, S.-H., Cho, H.-S. and Kim, K.-H. 2011. Development of RT-PCR based method for detecting five non-reported quarantine plant viruses infecting the family Cucurbitaceae or Solanaceae. Plant Pathol. J. 27:93-97. https://doi.org/10.5423/PPJ.2011.27.1.093
  10. Lee, B. Y., Lim, H. R., Choi, J. Y. and Ryu, K. H. 2004. Development of molecular detection of three species of seed-transmissible viruses useful for plant quarantine. Plant Pathol. J. 20: 302-307. https://doi.org/10.5423/PPJ.2004.20.4.302
  11. Lian, S., Jonson, M. G., Cho, W. K., Choi, H.-S., Je, Y. H. and Kim, K.-H. 2011. Generation of antibodies against Rice stripe virus proteins based on recombinant proteins and synthetic polypeptides. Plant Pathol. J. 27:37-43. https://doi.org/10.5423/PPJ.2011.27.1.037
  12. Park, M.-R. and Kim, K.-H. 2004. RT-PCR detection of three non-reported fruit tree viruses useful for quarantine purpose in Korea. Plant Pathol. J. 20:147-154. https://doi.org/10.5423/PPJ.2004.20.2.147
  13. Pappu, H. R., du Toit, L. J., Schwartz, H. F. and Mohan, S. K. 2006. Sequence diversity of the nucleoprotein gene of iris yellow spot virus (genus tospovirus, family bunyaviridae) isolates from the western region of the united states. Arch. Virol. 151:1015-1023. https://doi.org/10.1007/s00705-005-0681-z
  14. Salamon, P. and Palkovics, L. 2005. Occurrence of Colombian datura virus in Brugmansia hybrids, Physalis peruviana l. and Solanum muricatum Ait. in Hungary. Acta Virol. 49:117-122.
  15. Sreenivasulu, M. and Sai Gopal, D. V. R. 2010. Development of recombinant coat protein antibody based IC-RT-PCR and comparison of its sensitivity with other immunoassays for the detection of papaya ringspot virus isolates from India. Plant Pathol. J. 26:25-31. https://doi.org/10.5423/PPJ.2010.26.1.025
  16. Zhang, Y., Zhao, W., Li, M., Chen, H., Zhu, S. and Fan, Z. 2011. Real-time taqman RT-PCR for detection of maize chlorotic mottle virus in maize seeds. J. Virol. Methods 171:292-294. https://doi.org/10.1016/j.jviromet.2010.11.002

피인용 문헌

  1. Development and of Diagnostic System for Detection of Cowpea chlorotic mottle virus using by Nested PCR vol.41, pp.4, 2014, https://doi.org/10.7744/cnujas.2014.41.4.335
  2. First Report of Iris yellow spot virus Infecting Onion in Zimbabwe vol.100, pp.1, 2016, https://doi.org/10.1094/PDIS-07-15-0814-PDN
  3. Development and Practical Use of RT-PCR for Seed-transmitted Prune dwarf virus in Quarantine vol.30, pp.2, 2014, https://doi.org/10.5423/PPJ.NT.10.2013.0099
  4. Development of PCR Diagnostic System for Detection of the Seed-Transmitted Tobacco Ringspot Virus in Quarantine vol.55, pp.2, 2015, https://doi.org/10.1007/s12088-015-0518-8
  5. Iris yellow spot virus in Zimbabwe: Incidence, severity and characterization of Allium-infecting isolates vol.94, 2017, https://doi.org/10.1016/j.cropro.2016.12.019
  6. Highly Specific Detection of Five Exotic Quarantine Plant Viruses using RT-PCR vol.29, pp.1, 2013, https://doi.org/10.5423/PPJ.NT.09.2012.0142
  7. First Report of Iris yellow spot virus Infecting Garlic and Leek in Zimbabwe vol.100, pp.3, 2016, https://doi.org/10.1094/PDIS-09-15-1022-PDN
  8. Development of PCR Diagnosis System for Plant Quarantine Seed-borne Wheat Streak Mosaic Virus vol.49, pp.2, 2013, https://doi.org/10.7845/kjm.2013.3013
  9. Development of a PCR Diagnostic System for Iris yellow spot tospovirus in Quarantine vol.30, pp.4, 2014, https://doi.org/10.5423/PPJ.NT.06.2014.0052
  10. Detection of Carnation necrotic fleck virus and Carnation ringspot virus Using RT-PCR vol.19, pp.1, 2013, https://doi.org/10.5423/RPD.2013.19.1.036
  11. Development of RT-PCR and Nested PCR for Detecting Four Quarantine Plant Viruses Belonging to Nepovirus vol.19, pp.3, 2013, https://doi.org/10.5423/RPD.2013.19.3.220
  12. Development of a PCR Diagnostic System for Detecting Andean Potato Mottle Virus Associated with Potato Quarantine in Korea vol.92, pp.4, 2015, https://doi.org/10.1007/s12230-015-9468-2
  13. Development and Optimization of a Reverse Transcription Hemi-Nested PCR Primer for the Detection of Potato Mop-Top Virus at Quarantine Inspection Sites in Korea vol.57, pp.2, 2017, https://doi.org/10.1007/s12088-016-0623-3
  14. Development of Nucleotide Primers for Dignostic RT-PCR and Nested PCR Detection of Three Seed-transmitted Viruses (CRLV, SpLV and WClMV) in Quarantine vol.48, pp.3, 2014, https://doi.org/10.14397/jals.2014.48.3.75