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Control Efficacy of Fungicides on Chinese Cabbage Clubroot under Several Conditions

발병 조건에 따른 살균제들의 배추 뿌리혹병 방제효과

  • Eom, Min-Yong (Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology) ;
  • Jo, Su-Jung (Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology) ;
  • Jang, Kyoung-Soo (Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology) ;
  • Choi, Yong-Ho (Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology) ;
  • Kim, Jin-Cheol (Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology) ;
  • Choi, Gyung-Ja (Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology)
  • 엄민용 (한국화학연구원 산업바이오화학연구센터) ;
  • 조수정 (한국화학연구원 산업바이오화학연구센터) ;
  • 장경수 (한국화학연구원 산업바이오화학연구센터) ;
  • 최용호 (한국화학연구원 산업바이오화학연구센터) ;
  • 김진철 (한국화학연구원 산업바이오화학연구센터) ;
  • 최경자 (한국화학연구원 산업바이오화학연구센터)
  • Received : 2011.06.16
  • Accepted : 2011.07.13
  • Published : 2011.08.31

Abstract

To develop the efficient screening methods for antifungal compound active to Chinese cabbage clubroot caused by Plasmodiophora brassicae, the control efficacy of three fungicides fluazinam, ethaboxam, and cyazofamid on the disease was tested under several conditions such as soil types, cultivars of Chinese cabbage, growth stages of the host, and inoculum concentrations. The in vivo antifungal activities of the fungicides on clubroot of two Chinese cabbage cultivars were hardly different. At 7- and 14-day-old seedlings, the fungicides were more effective to control of clubroot than at 21-day-old seedlings. In a commercial horticulture media soil (CNS), disease severity of untreated controls was higher and control activity of the fungicides was less than in a mixture of CNS and upland soil (1:1, v/v). Disease development of the seedlings inoculated with P. brassicae at $1.8{\times}10^7$ spores/pot to $1.1{\times}10^9$ spores/pot was almost same, but control efficacy of the fungicides was negatively correlated with inoculum dosages. To effectively select in vivo antifungal compound on Chinese cabbage clubroot, 14-day-old seedlings need to be inoculated with P. brassicae by drenching the spore suspension to give $1{\times}10^8$ spores/pot 1 day after chemical treatment. To develop clubroot, the inoculated plants are incubated in a growth chamber at $20^{\circ}C$ for 2 days, and then cultivated in a greenhouse ($20{\pm}5^{\circ}$) for four weeks.

배추 뿌리혹병에 대한 효율적인 살균 활성 검정법을 확립하기 위하여, 배추 품종, 기주 식물의 생육 시기, 접종원 농도, 토양 종류 등의 발병 조건에 따른 살균제 fluazinam, ethaboxam 및 cyazofamid의 배추 뿌리혹병에 대한 방제 효과를 온실에서 실험하였다. 배추의 생육 시기에 따른 살균제들의 방제효과는 파종 7일 및 14일 유묘에서는 21일 유묘보다 더 높은 방제효과를 보였다. 그리고 토양종류에 따른 살균제의 방제효과는 원예용 상토보다 혼합토양에서 더 높은 방제효과를 나타냈다. 무처리구의 뿌리혹병 발생은 배추가 커질수록 증가하였으며, 혼합토양보다 원예용 상토에서 병 발생이 더 많았다. 그러나 배추품종에 따른 살균제들의 방제효과 차이는 거의 없었다. 한편 P. brassicae 포자 농도가 100배 증가하여도 무처리구의 뿌리혹병 발생은 거의 차이가 없었으나, 살균제들의 배추 뿌리혹병 방제효과는 크게 감소하였다. 이상의 결과로부터 살균제 fluazinam, ethaboxam 및 cyazofamid는 토양 종류, 배추 생육시기 및 접종원 농도 등의 발병 조건에 따라 배추 뿌리혹병에 대하여 다양한 방제효과를 보였으며, 이들 중 포자 농도가 살균제의 방제효과에 가장 큰 영향을 미친다고 생각되었다.

Keywords

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