Journal of the Korean Society for Marine Environment & Energy (한국해양환경ㆍ에너지학회지)

The Korean Society for Marine Environment and Energy (한국해양환경•에너지학회)
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Proposal of Standard Method for the Analysis of Polycyclic Aromatic Hydrocarbons in Marine Sediments

해양퇴적물내 다환방향족탄화수소 분석을 위한 표준작업절차서 제안

  • Lee, Hyo-Jin (Department of marine Environmental Engineering, Institute of Marine Industry, Gyeongsang National University) ;
  • Kim, Gi-Beum (Department of marine Environmental Engineering, Institute of Marine Industry, Gyeongsang National University) ;
  • Choi, Yoon-Seok (Tidal-Flat Research Center)
  • 이효진 (경상대학교 해양과학대학 해양환경공학과 해양산업연구소) ;
  • 김기범 (경상대학교 해양과학대학 해양환경공학과 해양산업연구소) ;
  • 최윤석 (국립수산과학원 갯벌연구소)
  • Received : 2011.03.22
  • Accepted : 2011.08.11
  • Published : 2011.08.25
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Abstract

Though polycyclic aromatic hydrocarbon compounds (PAHs) in marine sediment has been produced by many colleges and institution in Korea, it is difficult to compare PAHs data in a study area with those in other areas due to the lack of confidence for the quality of data from the other organization. Therefore, we suggested the protocol for PAHs analysis in marine sediment through examining the method of PAHs analysis described in over twenty scientific papers and reports. When a known amount of 23 PAHs were spiked into a sediment and anlyzed following this new protocol, very good recoveries were obtained. In addition, for college and institution with their own method to analyze PAHs can keep producing PAHs data without exchanging to this new PAHs protocol, the method to get a full confidence through the QA/QC for the PAHs data produced by these organization is included to the protocol.

현재 국내의 수많은 연구기관과 대학에서 다양한 화학분석법과 기기를 이용하여 다환방향족탄화수소(polycyclic aromatic hydrocarbons, PAHs)자료를 산출해 내고 있으나, 자료에 대한 엄격한 정도관리가 이루어지지 않은 관계로 자료를 산출한 상대 기관에 대한 신뢰가 부족하고, 농도에 대한 절대 비교가 불가능한 경우가 많았다. 그러므로 본 연구에서는 최신의 분석방법으로 국내외학술지 및 연구보고서에 게재되어진 20여 편의 참고문헌으로부터 보편적으로 받아들여지는 분석방법과 자료질을 조사하여 이를 반영한 표준작업 절차서를 제시하였다. 이 절차서에 따라 표준물질 첨가법으로 회수율을 검정하였을 때 좋은 회수율 값을 보여주었다. 또한 PAHs 분석하고 있는 기존의 연구 기관들의 경우 굳이 실험법을 바꾸지 않더라도 표준시료의 사용 등 환경정도관리를 통해 산출되어진 자료가 신뢰성을 획득할 수 있는 방안을 절차서 내에 포함하였다.

Keywords

References

  1. 국토해양부, 2010, 해양환경공정시험기준, pp. 495.
  2. 김창준, 홍기훈, 전지연, 김석현, 2010, "해양환경시료(해저퇴적물과 해양생물)에 축적된 석유계총탄화수소(TPHs) 분석에 대한 공정시험기준 제안", 한국해양환경공학회지 제13권 4호, 249-262.
  3. 해양경찰청, 2008, 해양경찰백서, pp. 523.
  4. Boonyatumanond, R., Wattayakorn, G., Togo, A. and Takada, H., 2006, Distribution and origins of polycyclic aromatic hydrocarbons (PAHs) in riverine, estuarine, and marine sediments in Thailand, Marine Pollution Bulletin, Vol. 52, 942-956. https://doi.org/10.1016/j.marpolbul.2005.12.015
  5. Cardellicchio, N., Buccolieri, A., Giandomenico, S., Loperz, L., Pizzulli, F. and Spada, L., 2007, Organic pollutants (PAHs, PCBs) in sediments from the Mar Piccolo in Taranto (Ionian Sea, Southern Italy), Marine Pollution Bulletin, Vol. 55, 451- 458. https://doi.org/10.1016/j.marpolbul.2007.09.007
  6. Culotta, L., Stefano, C.D., Gianguzza, A., Mannino, M.R. and Orecchio, S., 2006, The PAH composition of surface sediments from Stagnone coastal lagoon, Marsala (Italy), Marine Chemistry, Vol. 99, 117-127. https://doi.org/10.1016/j.marchem.2005.05.010
  7. Franco, M.A., Vinas, L., Soriano, J.A., de Armas, D., Gonzalez, J.J., Beiras, R., Salas, N., Bayona, J.M. and Albaiges, J., 2006, Spatial distribution and ecotoxicity of petroleum hydrocarbons in sediments from the Galicia continental shelf (NW Spain) after the Prestige oil spill, Marine Pollution Bulletin, Vol. 53, 260- 271. https://doi.org/10.1016/j.marpolbul.2005.10.004
  8. Gaspare, L., Machiwa, J.F., Mdachi, S.J.M., Streck, G. and Brack, W., 2009, Polycyclic aromatic hydrocarbon (PAH) contamination of surface sediments and oysters from the inter-tidal areas of Dar es Salaam, Tanzania, Environmental Pollution, Vol. 157, 24-34. https://doi.org/10.1016/j.envpol.2008.08.002
  9. Gu, S.H., Kralovec, A.C., Christensen, E.R. and Van Camp, R.P., 2003, Source apportionment of PAHs in dated sediments from the Black River, Ohio, Water Research, Vol. 37, 2149- 2161. https://doi.org/10.1016/S0043-1354(02)00584-5
  10. Guo, W., He, M., Yang, Z., Lin, C., Quan, X. and Wang, H., 2007, Distribution of polycyclic aromatic hydrocarbons in water, suspended particulate matter and sediment from Daliao River watershed, China, Chemosphere, Vol. 68, 93-104. https://doi.org/10.1016/j.chemosphere.2006.12.072
  11. Houghton, J.P., Lees, D.C. and Driskell, W.B., 1991, Impacts of the Exxon Valdez spill and subsequent cleanup on intertidal biota-1 year later. In: Proceeding of the 1991 International Oil Spill Conference: Prevention, Behavior, Control, Clean up. America Petroleum Institute, Washington, pp. 467-475.
  12. Iwano, S., Ichikawa, M., Takizawa, S., Hashimoto, H. and Miyamoto, Y., 2010, "Identification of AhR-regulated genes involved in PAH-induced immunotoxicity using a highly-sensitive DNA chip, 3D-$Gene^{TM}$HumanImmunity and Metabolic Syndrome9k", Toxicology in Vitro, Vol. 24, 85-91. https://doi.org/10.1016/j.tiv.2009.08.030
  13. Jackson, J.B.C., Cubit, J.D., Keller, B.D., Batista, V., Burns, K., Caffey, H.M., Caldwell, R.L., Garrity, S.D. and Getter, C.D., 1989, "Ecological effects of a major oil spill on Panamanian coastal marine communities", Science, Vol. 243, 37-44. https://doi.org/10.1126/science.243.4887.37
  14. Jiang, J.J., Lee, C.L., Fang, M.D. and Liu, J.T., 2009, Polycyclic aromatic hydrocarbons in coastal sediments of southwest Taiwan: An appraisal of diagnostic ratios in source recognition, Marine Pollution Bulletin, Vol. 58, 752-760. https://doi.org/10.1016/j.marpolbul.2008.12.017
  15. Kim, M., Kennicutt II, M.C. and Qian, Y., 2008, Source characterization using compound composition and stable carbon isotope ratio of PAHs in sediments from lakes, harbor, and shipping waterway, Science of the total environment, Vol. 389, 367-377. https://doi.org/10.1016/j.scitotenv.2007.08.045
  16. Law, R.J., Kelly, C.A., Baker, K.L., Langford, K.H. and Bartlett, T., 2002, "Polycyclic aromatic hydrocarbons in sediments, mussels and crustacea around a former gasworks site in Shorehamby- Sea, UK", Marine Pollution Bulletin, Vol. 44, 903-911. https://doi.org/10.1016/S0025-326X(02)00119-4
  17. Lemiere, S., Cossu-Leguille, C., Bispo, A., Jourdain, M.J., Lanhers, M.C., Burnel, D. and Vasseur, P., 2005, DNA damage measured by the single-cell gel electrophoresis (Comet) assay in mammals fed with mussels contaminated by the 'Erika' oilspill, Mutation Research, Vol. 581, 11-21. https://doi.org/10.1016/j.mrgentox.2004.10.015
  18. Luo, X.J., Chen, S.J., Mai, B.X., Yang, Q.S., Sheng, Q.Y. and Fu, J.M., 2006, Polycyclic aromatic hydrocarbons in suspended particulate matter and sediments from the Pearl River Estuary and adjacent coastal areas, China, Environmental Pollution, Vol. 139, 9-20. https://doi.org/10.1016/j.envpol.2005.05.001
  19. Macías-Zamora, J.V., Mendoza-Vega, E. and Villaescusa-Celaya, J.A., 2002, PAHs composition of surface marine sediments: a comparison to potential local sources in Todos Santos Bay, B.C., Mexico, Chemosphere, Vol. 46, 459-468. https://doi.org/10.1016/S0045-6535(01)00069-8
  20. Meador, J.P., Stein, J.E., Reichert, W.L. and Varanasi, U., 1995, "Bioaccumulation of polycyclic aromatic hydrocarbons by marine organisms", Reviews of Environmental Contamination & Toxicology, Vol. 143, 79-165. https://doi.org/10.1007/978-1-4612-2542-3_4
  21. Nunn, J.W., Livingstone, D.R. and Chipman, J.K., 1996, "Effect of genetic toxicants in aquatic organisms", Soc. Exp. Biol. Semin. Ser., Vol. 57, 225-251.
  22. Peterson, C.H., Rice, S.D., Short, J.W., Esler, D., Bodkin, J.L., Ballachey, B.E. and Irons, D.B., 2003, "Long-term ecosystem response to the Exxon Valdez oil spill", Science, Vol. 302, 2082- 2086. https://doi.org/10.1126/science.1084282
  23. Qiao, M., Wang, C., Huang, S., Wang, D. and Wang, Z., 2006, Composition, sources, and potential toxicological significance of PAHs in the surface sediments of the Meiliang Bay, Taihu Lake, China, Environment International, Vol. 32, 28-33. https://doi.org/10.1016/j.envint.2005.04.005
  24. Qiu, Y.W., Zhang, G., Liu, G.Q., Guo, L.L., Li, X.D. and Wai, O., 2009, Polycyclic aromatic hydrocarbons (PAHs) in the water column and sediment core of Deep Bay, South China, Estuarine, Coastal and Shelf Science, Vol. 83, 60-66. https://doi.org/10.1016/j.ecss.2009.03.018
  25. Saunders, C.R., Ramesh, A. and Shockley, D.C., 2002, "Modulation of neurotoxic behavior in F-344 rats by temporal disposition of benzo(a)pyrene", Toxicology Letters, Vol. 129, 33-45. https://doi.org/10.1016/S0378-4274(01)00467-2
  26. Scarpato, R., Migliore, L., Alfinito-Cogneti, G. and Barale, R., 2009, "Induction of micronuclei in gill tissue of Mytilus galloprovincialis exposed to polluted marine waters", Marine Pollution Bulletin, Vol. 21, 74-80.
  27. Sole, M., Porte, C., Biosca, X., Mitchelmore, C.L., Chipman, J.K., Livingstone, D.R. and Albaiges, J., 1996, "Effects of the "Aegean Sea" oil spill on biotransformation enzymes, oxidative stress and DNA adducts in digestive gland of the mussel (Mytilus edulis L.)", Comparative Biochemistry and Physiology, Vol. 113C, 257-265.
  28. Unlu, S. and Alpar, B., 2006, Distribution and sources of hydrocarbons in surface sediments of Gemlik Bay (Marmara Sea, Turkey), Chemosphere, Vol. 64, 764-777. https://doi.org/10.1016/j.chemosphere.2005.10.064
  29. Wang, X.C., Zhang, Y.X. and Chen, R.F., 2001, Distribution and Partitioning of Polycyclic Aromatic Hydrocarbons (PAHs) in Different Size Fractions in Sediments from Boston Harbor, United States, Marine Pollution Bulletin, Vol. 42, 1139-1149. https://doi.org/10.1016/S0025-326X(01)00129-1
  30. White, P.A., 2002, 'The genotoxicity of priority polycyclic aromatic hydrocarbons in complex mixtures", Mutation Research, Vol. 515, 85-98. https://doi.org/10.1016/S1383-5718(02)00017-7
  31. Wu, Y., Zhang, J., Mi, T. and Li, B., 2001, Occurrence of nalkanes and polycyclic aromatic hydrocarbons in the core sediments of the Yellow Sea, Marine Chemistry, Vol. 76, 1-15. https://doi.org/10.1016/S0304-4203(01)00040-8
  32. Xu, J., Ping, Y.Y., Guo, W., Dai, S. and Sun, H., 2007, Polycyclic aromatic hydrocarbons in the surface sediments from Yellow River, China, Chemosphere, Vol. 67, 1408-1414. https://doi.org/10.1016/j.chemosphere.2006.10.074
  33. Yan, W., Chi, J., Wang, Z., Huang, W. and Zhang, G., 2009, Spatial and temporal distribution of polycyclic aromatic hydrocarbons (PAHs) in sediments from Daya Bay, South China, Environmental Pollution, Vol. 157, 1823-1830. https://doi.org/10.1016/j.envpol.2009.01.023
  34. Yim, U.H., Hong, S.H. and Shim, W.J., 2007, "Distribution and characteristics of PAHs in sediments from the marine environment of Korea", Chemosphere, Vol. 68, 85-92. https://doi.org/10.1016/j.chemosphere.2006.12.032
  35. Yim, U.H., Hong, S.H., Shim, W.J., Oh, J.R. and Chang, M., 2005, Spatio-temporal distribution and characteristics of PAHs in sediments from Masan Bay, Korea, Marine Pollution Bulletin, Vol. 50, 319-326. https://doi.org/10.1016/j.marpolbul.2004.11.003
  36. Zhou, J.L. and Maskaoui, K., 2003, Distribution of polycyclic aromatic hydrocarbons in water and surface sediments from Daya Bay, China, Environmental Pollution, Vol. 121, 269-281. https://doi.org/10.1016/S0269-7491(02)00215-4