Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Association of the KAP 8.1 Gene Polymorphisms with Fibre Traits in Inner Mongolian Cashmere Goats

  • Liu, Haiying (College of Animal Science and Veterinary Science, Shenyang Agricultural University) ;
  • Yue, Chun-Wang (College of Animal Science and Technology, Hebei North University) ;
  • Zhang, Wei (College of Animal Science and Technology, China Agricultural University) ;
  • Zhu, Xiaoping (College of Animal Science and Technology, China Agricultural University) ;
  • Yang, Guiqin (College of Animal Science and Veterinary Science, Shenyang Agricultural University) ;
  • Jia, Zhihai (College of Animal Science and Technology, China Agricultural University)
  • Received : 2011.04.25
  • Accepted : 2011.06.11
  • Published : 2011.10.01
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Abstract

The objective of this study was to investigate polymorphisms of keratin-associated protein 8.1 (KAP8.1) gene and its effect on fibre traits of Chinese Inner Mongolian Cashmere goats. The fibre traits data investigated were cashmere fibre diameter, combed cashmere weight, cashmere fibre length and guard hair length. Five hundred and forty animals were used to detect polymorphisms in the complete coding sequence of the hircine KAP8.1 gene by means of PCR-SSCP. The results identified six genotypes, AA, BB, CC, AB, AC and BC, coded for by three different alleles A, B and C. Two SNPs in the coding region were confirmed by sequencing, which were T113G and G116C respectively. The relationships between the genotypes and cashmere fibre diameter, combed cashmere weight, cashmere fibre length and guard hair length were analyzed. There were significant differences between the associations of the different genotypes with cashmere weight (p<0.01), cashmere length (p<0.05) and hair length (p<0.01). Cashmere fibre diameter was the only trait that was not associated with the genotypes. The animals of genotype AB and BB had the higher cashmere weight compared with the genotype AA. By further analysis, it appeared that the KAP8.1 genotype effects on fibre traits may be due to a mutation at the 113 locus. These results suggested that polymorphisms in the hircine KAP8.1 gene might be a potential molecular marker for cashmere weight in Cashmere goats.

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References

  1. Adelson, D. L., D. E. Hollis and G. H. Brown. 2002. Wool fibre di ameter and follicle density are not specified simultaneously during wool follicle initiation. Aust. J. Agric. Res. 53:1003-1009. https://doi.org/10.1071/AR01200
  2. Bai, J. Y., Q. Zhang, J. Q. Li, Er-Ji Dao and X. P. Jia. 2006. Estimates of genetic parameters and genetic trends for production traits of Inner Mongolian White Cashmere goat. Asian-Aust. J. Anim. Sci. 19(1):13-18.
  3. Beh, K. J., M. J. Callaghan, Z. Leish, D. J. Hulme, I. Lenane and J. F. Maddox. 2001. A genome scan for QTL affecting fleece and wool traits in Merino sheep. Wool Technol Sheep Breed. 49:88-89.
  4. Chamary, J. V. and L. D. Hurst. 2005. Evidence for selection on synonymous mutations affecting stability of mRNA secondary structure in mammals. Genome Biol. 6:R75. https://doi.org/10.1186/gb-2005-6-9-r75
  5. Frenkel, M. J., J. M. Gillespie and P. J. Reis. 1974. Factors influencing the biosynthesis of the tyrosine-rich proteins of wool. Aust. J. Biol. Sci. 27:31-38.
  6. Gillespie, J. M. 1972. Proteins rich in glycine and tyrosine from keratins. Comp. Biochem. Physiol. B. 41(4):723-734. https://doi.org/10.1016/0305-0491(72)90085-5
  7. Gillespie, J. M. 1990. The proteins of hair and other hard $\alpha$-keratins. In: Cellular and Molecular Biology of Intermediate Filaments (Ed. R. D. Goldman and P. M. Steinert). pp. 95-128. Plenum Press, New York.
  8. Itenge-Mweza, T. O., R. H. J. Forrest, G. W. McKenzie, A. Hogan, J. Abbott, O. Amoafo and J. G. H. Hickford. 2007. Polymorphism of the KAP1.1, KAP1.3 and K33 genes in Merino sheep. Mol. Cell. Probes. 21:338-342. https://doi.org/10.1016/j.mcp.2007.04.002
  9. Komar, A. A. 2007. Silent SNPs: impact on gene function and phenotype. Pharmacogenomics 8(8):1075-1080. https://doi.org/10.2217/14622416.8.8.1075
  10. Liu, H. Y., N. Li, C. L. Jia, X. P. Zhu and Z. H. Jia. 2007. Effect of the polymorphisms of keratin associated protein 8.2 gene on fibre traits in inner Mongolia cashmere goats. Asian-Aust. J. Anim. Sci. 20(6):821-827. https://doi.org/10.5713/ajas.2007.821
  11. McCarthy, B. J. 1998. Specialty animal fibres. Textiles 1:6-8.
  12. McKenzie, G., A. P. Maher, K. G. Dodds, A. E. Beattie, H. M. Henry, J. G. H. Hickford and G. W. Montgomery. 2001. The search for quantitative trait loci affecting wool colour. Proc. N.Z. Soc. Anim. Prod. 61:104-108.
  13. McLaren, R. J., G. R. Rogers, K. P. Davies, J. F. Maddox and G. W. Montgomery. 1997. Linkage mapping of wool keratin and keratin-associated protein genes in sheep. Mamm. Genome 8:938-940. https://doi.org/10.1007/s003359900616
  14. Parsons, Y. M., D. W. Cooper and L. R. Piper. 1994. Evidence of linkage between high-glycine-tyrosine keratin gene loci and wool fibre diameter in a merino half-sib family. Anim. Genet. 25:105-108. https://doi.org/10.1111/j.1365-2052.1994.tb00414.x
  15. Powell, B. C. and G. E. Rogers. 1997. The role of keratin proteins and their genes in the growth, structure and properties of hair. In Formation and structure of human hair (Ed. P. Jolles, H. Zahn, H. Hocker) pp 59-148. Birkhauser verlag, Basel.
  16. Rogers, G. R., J. G. H. Hickford and R. Bickerstaffe. 1994. A potential QTL for wool strength located on ovine chromosome 11. In: Proceedings of the fifth World congress on genetics applied to livestock production, vol. 21. p. 291-294.
  17. Salomons, G. S., L. A. Bok, E. A. Struys, L. L. Pope, P. S. Darmin, P. B. Mills, P. T. Clayton, M. A. Willemsen and C. Jakobs. 2007. An intriguing "silent" mutation and a founder effect in antiquitin (ALDH7A1). Ann. Neurol. 62:414-418. https://doi.org/10.1002/ana.21206
  18. Sambrool, J., E. F. Fritsch and T. Maniatis. 1989. Molecular Cloning: a laboratory manual. 2nd ed. N.Y., Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory Press.
  19. Shah, J. H., D. J. Maguire, T. B. Munce and A. Cotterill. 2008. Alanine in HI: a silent mutation cries out! Adv. Exp. Med. Biol. 614:145-150. https://doi.org/10.1007/978-0-387-74911-2_17
  20. Shimomura, Y., N. Aoki, J. Schweizer, L. Langbein, M. A. Rogers, W. Hermelita and M. Ito. 2002. Polymorphisms in the human high sulfur hair keratin-associated protein 1, KAP1, gene family. J. Biol. Chem. 277(47):45493-45501. https://doi.org/10.1074/jbc.M206398200
  21. Wood, N. J., S. H. Phua and A. M. Crawford. 1992. A dinucleotide repeat polymorphism at the glycine-rich and tyrosine-rich keratin locus in sheep. Anim. Genet. 23:391.
  22. Yu, H., X. Wang, H. Chen, M. Wang, M. Zhao, X. Y. Lan, C. Z. Lei, K. Y. Wang, X. S. Lai and X. L. Wang. 2008. The polymorphism of a novel 30 bp-deletion mutation at KAP9.2 locus in the cashmere goat. Small. Rumin. Res. 80(1):111-115. https://doi.org/10.1016/j.smallrumres.2008.09.010
  23. Zhao, J. X., Y. S. Ren and W. B. Yue. 2007. Analysis of polymorphism on KAP 8 gene in three goat breeds with PCR-SSCP. Biotechnology 17(5):3-6.
  24. Zhao, M., H. Chen, X. Wang, H. Yu, M. Wang, J. Wang. X. Y. Lan, C. F. Zhang, L. Z. Zhang, Y. K. Guo, B. Zhang and S. R. Hu. 2009. aPCR-SSCP and DNA sequencing detecting two silent SNPs at KAP8.1 gene in the cashmere goat. Mol. Biol. Rep. 36(6):1387-1391. https://doi.org/10.1007/s11033-008-9325-1
  25. Zhou, H. M., D. Allain, J. Q. Li, W. G. Zhang and X. C. Yu. 2003. Effects of non-genetic factors on production traits of Inner Mongolia cashmere goats in China. Small. Rumin. Res. 47:85-89. https://doi.org/10.1016/S0921-4488(02)00246-8

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