Journal of Pharmaceutical Investigation

The Korean Society of Pharmaceutical Sciences and Technology (한국약제학회)
권호 표지
DOI QR코드

DOI QR Code

Quantitative Analysis and Preformulation of Extracts from Alnus Japonica

  • Baek, Jong-Suep (College of Pharmacy and Institute of Drug Research and Development, Chungnam National University) ;
  • Kang, Hee-Chul (College of Pharmacy and Institute of Drug Research and Development, Chungnam National University) ;
  • Keum, Chang-Gu (College of Pharmacy and Institute of Drug Research and Development, Chungnam National University) ;
  • Lim, Ji-Ho (College of Pharmacy and Institute of Drug Research and Development, Chungnam National University) ;
  • Hwang, Chan-Ju (College of Pharmacy and Institute of Drug Research and Development, Chungnam National University) ;
  • Na, Young-Guk (College of Pharmacy and Institute of Drug Research and Development, Chungnam National University) ;
  • Tung, N.H. (College of Pharmacy and Institute of Drug Research and Development, Chungnam National University) ;
  • Kim, Young-Ho (College of Pharmacy and Institute of Drug Research and Development, Chungnam National University) ;
  • Cho, Cheong-Weon (College of Pharmacy and Institute of Drug Research and Development, Chungnam National University)
  • Received : 2011.05.09
  • Accepted : 2011.07.01
  • Published : 2011.08.20
Download PDF
⟨ Previous Next ⟩

Abstract

Alnus japonica has been known to exert antioxidative, anti-inflammatory, anti-cancer and immune response inhibitory effects. The aim of this study was to figure out the characteristics of extracts obtained with different extraction solvent such as water, 100% ethanol, 70% ethanol or 70% methanol because characteristic components such as oregonin and hirsutanone extracted from Alnus japonica might be essential for the biological activity. For this purpose, oregonin and hirsutanon of four extracts, index ingredient of Alnus japonica, were analyzed with HPLC and physicochemical studies such as SEM, particle size and zeta potential were conducted. In cell cytotoxicity study, extract of water showed the highest cytotoxicity among four extracts. In case of oregonin, 70% MeOH and water extracts showed high contents and in case of hirsutanone, all extracts showed similar contents except 70% EtOH extracts. The extract of 70% MeOH from Alnus japonica for both oregonin and hirsutanone appeared to have the highest content. Both oregonin and hirsutanone extracted from Alnus japonica using 70% methanol showed stability in pH 1.2.

Keywords

References

  1. Lee, S.J., 1996. Korea folk medicine, Seoul National University Publishing Center Press, Seoul. 40.
  2. Kehrer, J.P., 1993. Free radicals as mediators of tissue injury and disease. Crit. Rev. Toxicol. 23, 21-48. https://doi.org/10.3109/10408449309104073
  3. Kim, J.H., Lee, K.W., Lee, M.W., Lee, H.J., Kim, S.H., 2006. Hirsutenone inhibits phorbol ester-induced upregulation of COX-2 and MMP-9 in cultured human mammary epithelial cells: NF-kappaB as a potential molecular target. FEBS Lett. 580, 385-392. https://doi.org/10.1016/j.febslet.2005.12.015
  4. Masanori K, Mari S, Yasuo N, Norio M, Takuro O, Nobuo K, Takahisa N, Toshikazu S. 2005. New diarylheptanoids from Alnus japonica and their antioxidative activity. Chem Pharm Bull 53, 1519-1523. https://doi.org/10.1248/cpb.53.1519
  5. Choi, S.E., Jeong, M.S., Kang, M.J., Lee, D.I., Joo, S.S., Lee, C.S., Bang, H., Lee, M.K., Myung, S.C., Choi, Y.W., Lee, K.S., Seo, S.J., Lee, M.W., 2010. Effect of topical application and intraperitoneal injection of oregonin on atopic dermatitis in NC/Nga mice. Exp. Dermatol. 8, e37-43.
  6. Lee, M.W., Kim, J.H., Jeong, D.W., Ahn, K.H., Toh, S.H., Surh, Y.J., 2000. Inhibition of cyclooxygenase-2 expression by diarylheptanoids from the bark of Alnus hirsuta var. sibirica. Biol. Pharm. Bull. 23, 517-518. https://doi.org/10.1248/bpb.23.517
  7. Cho, S.M., Kwon, Y.M., Lee, J.H., Yon, K.H., Lee, M.W., 2002. Melanogenesis inhibitory activities of diarylheptanoids from Alnus hirsuta Turcz in B16 mouse melanoma cell. Arch. Pharm. Res. 25, 885-888. https://doi.org/10.1007/BF02977009
  8. Kang, G., Kong, P.J., Yuh, S.V., Chun, W., Kim, S.S., 2004. Curcumin suppresses lipopolysaccharide-induced cyclooxygenase- 2 expression by inhibiting activator protein 1 and nuclear factor kappab bindings in BV2 microglial cells. J. Pharmacol. Sci. 94, 325-328. https://doi.org/10.1254/jphs.94.325
  9. Lee, M.W., Kim, N.Y., Park, M.S., 2000. Diarylheptanoids with in vitro inducible nitric oxide synthesis ibhibitory activity from Alnus hirsuta. Planta Med. 66, 551-553. https://doi.org/10.1055/s-2000-8606
  10. Kim, H.J., Yeom, S.H., Kim, M.K., Shim, J.G., Paek, I.N., Lee, M.W., 2005. Nitric oxide and prostaglandin E2 synthesis ibhibitory activities of diarylheptanoids from the barks of Alnus japonica steudel. Arch. Pharm. Res. 28, 177-179. https://doi.org/10.1007/BF02977711
  11. Choi, E.J., Ko, H.H., Lee, M.W., Bang, H.W., Lee, .C.S., 2008. Inhibition of activated responses in dendritic cells exposed to lipopolysacchardie and lipoteichoic acid by diarylheptanoid oregonin. Int. Immunopharmacol. 8, 748-755. https://doi.org/10.1016/j.intimp.2008.01.024
  12. Carstensen, J.T., 2001. Advanced Pharmaceutical Solids. New York: Marcel Dekker, 51-191.
  13. Kuroyanagi, M., Shimomae, M., Nagashima, Y., Nuro, N., Okuda, T., Kawahara, N., Najane, T., Sano, T., 2005. New diarylheptanoids from Alnus japonica and their antioxidative activity. Chem. Pharm. Bull. 53, 1519-1523. https://doi.org/10.1248/cpb.53.1519

Cited by

  1. Natural Dyeing of Ramie Fabrics with Acer Ginnala, Alnus Japonica and Gromwell Extracts vol.18, pp.4, 2014, https://doi.org/10.12940/jfb.2014.18.4.15