Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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In vitro Activities of Polycalcium, a Mixture of Polycan and Calcium Lactate-Gluconate, on Osteoclasts and Osteoblasts

In vitro에서 polycalcium 복합조성물이 파골세포와 조골세포에 미치는 영향

  • Choi, Jae-Suk (RIS Center, Industry-Academic Cooperation Foundation, Silla University) ;
  • Kim, Joo-Wan (Glucan Corp. Research Institute, Marine Bio-industry Development Center) ;
  • Kim, Ki-Young (Glucan Corp. Research Institute, Marine Bio-industry Development Center) ;
  • Cho, Hyung-Rae (Glucan Corp. Research Institute, Marine Bio-industry Development Center) ;
  • Ha, Yu-Mi (RIS Center, Industry-Academic Cooperation Foundation, Silla University) ;
  • Ku, Sae-Kwang (Department of Anatomy and Histology, College of Oriental Medicine, Daegu Haany University) ;
  • Cho, Kwang-Keun (Department of Animal Resources Technology, Gyeongnam National University of Science and Technology) ;
  • Choi, In-Soon (RIS Center, Industry-Academic Cooperation Foundation, Silla University)
  • Received : 2011.05.17
  • Accepted : 2011.07.27
  • Published : 2011.08.30
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Abstract

The present study evaluated the beneficial effects of polycalcium (a mixture of Polycan and calcium lactate-gluconate 1:9 [g/g]) on osteoporosis using in vitro assays. Cell proliferation and alkaline phosphatase activities of osteoblasts (human primary osteoblasts) and osteoclast differentiation of RAW264.7 cells (murine osteoclast progenitor cells) treated with different concentrations of polycalcium for various periods were assessed. Osteoblast proliferation was stimulated and prevented RANKL-induced osteoclast differentiation of RAW264.7 cells. These results support the development of ideal anti-osteoporotic agents, such as polycalcium, that exhibit properties that accelerate bone formation and inhibit bone resorption.

본 실험에서는 폴리칸(베타-글루칸)과 칼슘 락테이트 글루코네이트 1:9 (g/g) 복합 조성물인 Polycalcium의 시험관 내(in vitro) 골다공증에 대한 효과를 사람 유래 조골세포(human primary osteoblast)와 설치류 유래 파골 전구세포(raw264.7 cell)를 이용하여 평가하였다. Polycalcium이 조골세포에 미치는 영향을 확인한 결과, 10 mg/ml 농도의 polycalcium 처리군에서 무처리 대조군에 비해 유의성 있는 조골세포의 수적 증가가 각각 배양 3, 7 및 10일 후에 확인되었으며, 또한 10 mg/ml 농도의 polycalcium 처리군에서 무처리 대조군에 비해 유의성있는 ALP함량의 증가가 확인되었다. Polycalcium이 파골세포에 미치는 영향을 확인한 결과, 각각 $10^{-5}$, $10^{-3}$ 및 $10^{-1}$ mg/ml polycalcium 처리군에서 무처리 대조군에 비해 유의성 있는 파골세포의 수적 감소가 배양 4일 후에 확인되었다. 이 같은 결과를 바탕으로, polycalcium이 조골세포의 증식 촉진 효과와 함께 파골세포 형성 억제 효과가 있는 것으로 확인되었다.

Keywords

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