Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Characteristics on Equine Herpesvirus Type 3 from Korea

국내분리 말구진 원인병원체(Equine herpesvirus type 3)의 특징

  • Yang, Jae-Hyuk (Jeju Race Park, Korea Racing Authority) ;
  • Lim, Yoon-Kyu (College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University)
  • 양재혁 (한국마사회) ;
  • 임윤규 (제주대학교 수의과대학 및 수의과학연구소)
  • Received : 2011.05.11
  • Accepted : 2011.07.28
  • Published : 2011.08.30
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Abstract

Equine coital exanthema caused by equine herpesvirus type 3 (EHV-3) is a venereal disease which seriously drops horse reproduction rates. Here, we isolated EHV-3 from infected horses and investigated their biological characteristics. Initial cytopathic effects such as rounding of cells were detected 48 hours post infection of the virus into RK-13 cells. The infected cells were going to detach from the surface of culture flasks 72 hours post infection. The type of isolated viruses from swabbed samples was EHV-3 by PCR analysis. Glycoprotein G (gG) of isolated EHV-3 has a 99.25 percent similarity rate to that of EHV-3 334/74 control strain. The isolated EHV-3 was named Georo strain. Georo strain consisted of four major proteins including 145 kD, 60 kD, 45 kD and 40 kD, as shown by SDS-PAGE analysis. We hope the newly isolated Georo strain of EHV-3 can be used for studying various aspects of Korean equine coital exanthema.

Equine herpesvirus type 3 (EHV-3)는 말[마(馬)]에서 말구진을 유발하고 말산업에 치명적인 악영향을 끼치는 매우 중요한 병원체이다. 국내에서 처음으로 말구진이 발생하였고, 말 생산농가에 경제적 피해를 입혔으나 다행히 원인바이러스 분리에 성공하였다. 이에 원인바이러스의 생물학적 특징을 연구하여 다음과 같은 결과를 얻었다. EHV-3를 RK-13 cell에 접종한 결과 접종 48 시간부터 세포변성효과가 나타남을 확인하였고 접종 72 시간부터는 세포박리가 일어났다. 세포를 수확하여 바이러스 DNA를 추출한 후 PCR 검사를 수행하였다. 그 결과 EHV-3에만 양성이었고 EHV-1 및 EHV-4에는 음성이었다. Swab에서 바이러스 DNA를 추출하여 10배씩 serial dilution을 한 후 PCR 검사를 실시한 결과 $10^4$까지 밴드를 확인할 수 있었다. 국내분리 EHV-3의 gG 특정부위에 대한 염기에서 표준주인 EHV-3 334/74 strain과 397개가 일치하여 99.25%의 상동성을 나타내었고 국내분리주를 EHV-3 거로주(Georo strain)라 명명하였다. 전기영동 결과에서는 EHV-3가 EHV-1보다 적은 수의 밴드를 확인할 수 있었다. 국내 분리주 EHV-3 거로주의 단백질을 분석한 결과 145 kD, 60 kD, 45 kD, 40 kD에서 단백 밴드를 관찰하였다.

Keywords

References

  1. Borchers, K. and J. Slater. 1993. A nested PCR for the detection and differentiation of EHV-1 and EHV-4. J. Virol. Methods 45, 331-336. https://doi.org/10.1016/0166-0934(93)90117-A
  2. Bryans, J. T. and G. P. Allen. 1973. In vitro and in vivo studies of equine coital exanthema, pp. 322-336, In Bryans, J. T. and H. Gerber (eds.), Equine Infectious Diseases III. S. Karger, New York.
  3. Cho, G. J., B. H. Kim, D. S. Lee, M. Y. Oh, and M. H. Ko. 1995. Studies on characters and immunogenicity of equine herpesvirus isolated from Korean horses. Korean J. Vet. Res. 35, 743-752.
  4. Colahan, P. T., A. M. Merritt, J. N. Moore, and I. G. Mayhew. 1999. Equine medicine and surgery. pp. 1071-1921, 5th eds., Mosby, Missouri.
  5. Crandell, R. A. and E. R. Davis. 1985. Isolation of equine coital exanthema virus (equine herpesvirus 3) from the nostril of a foal. J. Am. Vet. Med. Assoc. 187, 503-504.
  6. Fields, B. N., D. M. Knipe, R. M. Chanock, P. M. Howley, J. L. Melnick, T. P. Monath, B. Roizman, and S. E. Straus. 1995. Fundamental virology. pp. 1043-1107, 3rd eds., Lippincott-Raven, New York.
  7. Fukushi, H., A. Tanaiguchi, K. Yasuda, T. Yanai, T. Masegi, T. Yamaguchi, and K. Hirai. 2000. A hamster model of equine herpesvirus 9 induced encephalitis. J. Neurovirol. 6, 314-319. https://doi.org/10.3109/13550280009030757
  8. Girard, A., A. S. Greig, and D. Mitchell. 1968. Virus associated with vulvitis and balanitis in the horse-a preliminary report. Can. J. Comp. Med. 32, 603-604.
  9. Hartley, C. A., H. E. Drummer, and M. J. Studdert. 1999. The nucleotide sequence of the glycoprotein G homologue of equine herpesvirus 3 (EHV3) indicates EHV3 is a distinct equid alphaherpesvirus. Arch. Virol. 144, 2023-2033. https://doi.org/10.1007/s007050050723
  10. Jacob, R. J. 1986. Molecular pathogenesis of equine coital exanthema: Temperature-sensitive function(s) in cells infected with equine herpesviruses. Vet. Microbiol. 11, 221-237. https://doi.org/10.1016/0378-1135(86)90025-8
  11. Jacob, R. J. and M. R. Steiner. 1986. Molecular pathogenesis of equine coital exanthema (ECE): Identification and expression of infected cell polypeptides at the restricted temperature during equine herpesvirus 3 infection. Vet. Microbiol. 18, 363-371.
  12. Kamada, M. and M. J. Studdert. 1983. Analysis of small and large plaque variants of equine herpesvirus type 3 by restriction endonucleases. Arch. Virol. 77, 259-264. https://doi.org/10.1007/BF01309273
  13. Kobluk, C. N., T. R. Ames, and R. J. Geor. 1995. The horse diseases & clinical management. pp. 557-985, 1st ed., Saunders, Philadelphia.
  14. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 15, 227, 680-685.
  15. Ostlund, E. N. 1993. The equine herpesvirus. Vet. Clin. North Am. Equine Pract. 9, 283-294.
  16. Pascoe, R. R., P. B. Spradbrow, and T. J. Bagust. 1969. An equine genital infection resembling coital exanthema associated with a virus. Aust. Vet. J. 45, 166-170. https://doi.org/10.1111/j.1751-0813.1969.tb01922.x
  17. Reed, S. M. and W. M. Bayly. 1998. Equine internal medicine. pp. 545-546, 1st ed. Saunders, Philadelphia.
  18. Roizman, B., R. C. Desrosiers, B. Fleckenstein, C. Lopez, A. C. Minson, and M. J. Studdert. 1992. The family herpesviridae: an update. Arch. Virol. 123, 425-449. https://doi.org/10.1007/BF01317276
  19. Samper, J. C. 2009. Equine breeding management and artificial insemination. pp. 103-161, 2nd eds., Saunders, Missouri.
  20. Seki, Y., T. M. Seimiya, G. Yaegashi, S. Kumagai, H. Sentsui, T. Nishimori, and R. Ishihara. 2004. Occurrence of equine coital exanthema in pastured Draft horses and isolation of equine herpesvirus 3 from progenital lesions. J. Vet. Med. Sci. 66, 1503-1508. https://doi.org/10.1292/jvms.66.1503
  21. Spear, P. G. 1993. Entry of alphaherpesviruses into cells. Seminars Virol. 4, 167-180. https://doi.org/10.1006/smvy.1993.1012
  22. Studdert, M. J., T. Simpson, and B. Roizman. 1981. Differentiation of respiratory and abortigenic isolates of equine herpesvirus 1 by restriction endonucleases. Science 214, 562-564. https://doi.org/10.1126/science.6270790
  23. Studdert, M. J. 1996. Virus infections of equines. pp. 39-46, 1st ed., Elsevier, Amsterdam.
  24. Sullivan, D. C., S. S. Atherton, J. Staczek, and D. J. O'Callaghan. 1984. Structure of the genome of equine herpesvirus type 3. Virology 132, 352-367. https://doi.org/10.1016/0042-6822(84)90041-2

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