한국약용작물학회지 (Korean Journal of Medicinal Crop Science)

  • 제19권4호
  • /
  • Pages.238-245
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  • 2011
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  • 1225-9306(pISSN)
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  • 2288-0186(eISSN)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
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흰민들레 에탄올 추출물 및 분획물의 항산화 및 항균활성과 Tyrosinase 저해활성

Antioxidative and Antibacterial Activity and Tyrosinase Inhibitory Activity of the Extract and Fractions from Taraxacum coreanum Nakai

  • 임도연 (광주여자대학교 미용과학과) ;
  • 이경인 (동신대학교 생물자원산업화지원센터)
  • Im, Do-Youn (Deptartment of Beauty Science, Kwangju Women's University) ;
  • Lee, Kyoung-In (Biotechnology Industrialization Center, Dongshin University)
  • 투고 : 2011.07.28
  • 심사 : 2011.08.13
  • 발행 : 2011.08.30
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초록

In this study, we investigated antioxidative activity, antibacterial activity against pathogenic strains including methicillin resistant Staphylococcus aureus (MRSA), and tyrosinase inhibitory activity in 75% ethanol extract of Taraxacum coreanum and its fractions. The total polyphenol and flavonoid contents of the extract were 238.59mg/g and 33.18mg/g and the total polyphenol and flavonoid contents of the ethyl acetate fraction were 427.81mg/g and 148.90mg/g as the highest content of fractions. In DPPH radical scavenging ability, $SC_{50}$ values of the ethyl acetate and butanol fraction were 38.40${\mu}g/ml$ and 82.28 ${\mu}g/ml$, respectively. In antibacterial activity by the disc diffusion assay against S. aureus, S. epidermidis and MRSA, the ethyl acetate fraction showed stronger antibacterial activity than other fractions and the extract. Especially, the ethyl acetate fraction was exhibited effective antibacterial activity against MRSA. In the cytotoxicity measurement by MTT assay, the extract and fractions were exhibited Raw 264.7 cell viabilities of 96.32~143.21% as nontoxic result in concentration of 5~100 ${\mu}g/ml$. As a result, the ethyl acetate fraction of the 75% ethanol extract from T. coreanum could be applicable to functional materials for related fields.

키워드

참고문헌

  1. Akashi T, Furuno T, Takahashi T and Ayabe SI. (1994). Biosynthesis of triterpenoids in cultured cells, and regenerated and wild plant organs of Taraxacum officinale. Phytochemistry. 36:303-308. https://doi.org/10.1016/S0031-9422(00)97065-1
  2. Blois MS. (1958). Antioxidant determinations by the use of a stable free radical. Nature. 181:1199-1200. https://doi.org/10.1038/1811199a0
  3. Choi SR, You DH, Kim JY, Park CB, Kim DH and Ryu J. (2009). Antioxidant activity of methanol extracts from Cudrania tricuspidata Bureau according to harvesting parts and time. Korean Journal of Medicinal Crop Science. 17:115-120.
  4. Chung HT, Pae HO, Choi BM, Billiar TR and Kim YM. (2001). Nitric oxide as a bioregulator of apoptosis. Biochemical and Biophysical Research Communications. 282:1075-1079. https://doi.org/10.1006/bbrc.2001.4670
  5. Fridovich I. (1989). Superoxide dismutase an adaption to paramagnetic gas. Journal of Biological Chemistry. 264:7761-7762.
  6. Haenen GR, Paquay JB, Korthhouwer RE and Bast A. (1997). Peroxinitrite scavenging by flavonoids. Biochemical and Biophysical Research Communications. 236:591-593. https://doi.org/10.1006/bbrc.1997.7016
  7. Han SH, Hwang JK, Park SN, Lee KH, Ko KI, Kim KS and Kim KH. (2005). Potential effect of solvent fractions of Taraxacum mongolicum H. on protection of gastric mucosa. Korean Journal of Food Science and Technology. 37:84-89.
  8. Heo SI and Wang MH. (2008). Antioxidant activity and cytotoxicity effect of extracts from Taraxacum mongolicum H. Korean Journal of Pharmacognosy. 39:255-259.
  9. Jung SW, Lee NK, Kim SJ and Han DS. (1995). Screening of tyrosinase inhibitor from plants. Korean Journal of Food Science and Technology 27:891-896.
  10. Kang MJ and Kim KS. (2001). Current trends of research and biological activities of dandelion. Food Industry and Nutrition. 6:60-67.
  11. Katrin S, Carles R and Schieber A. (2006). Taraxacum-A review on its phytochemical and pharmacological profile. Journal of Ethnopharmacology. 107:313-323. https://doi.org/10.1016/j.jep.2006.07.021
  12. Kim TW and Kim TH. (2011). Pancreatic lipase inhibitors in the roots of Taraxacum ohwianum, a herb used in korean traditional medicine. Korean Journal of Food Preservation. 18:53-58. https://doi.org/10.11002/kjfp.2011.18.1.053
  13. Lee HH and Lee SY. (2008). Cytotoxic and antioxidant effects of Taraxacum coreanum Nakai. and T. officinale WEB. extracts. Korean Journal of Medicinal Crop Science. 16:79-85.
  14. Lee KI, Choi CH, Kim SM and Pyo BS. (2010). Antibacterial activity and enhancing antibiotic effect of extract and fractions from Curcuma longa against MRSA strain. Korean Journal of Pharmacognosy. 41:38-42.
  15. Lee KI and Im DY. (2011). Biological activities of the water extract and its fractions from Taraxacum coreanum Nakai. Korean Journal of Pharmacognosy. 42:195-200.
  16. Lee KI, Yang SA, Pyo BS and Kim SM. (2011). Antibacterial activity against pathogens of acne and tyrosinase inhibitory activity of extract and fractions from bark of Prunus sargentii. Korean Journal of Pharmacognosy. 42:155-160.
  17. Lee MS. (2009). New antimicrobials on the horizon. Korean Journal of Medicine. 77:35-51.
  18. Ling J, Ha JH, Choi YY, Seo YC, Kim JS, Kim YO, Cha SW, Kim JC and Lee HY. (2011). Enhancement of cosmeceutical activities of Berberis koreana bark by high pressure and ultrasonification extraction processes. Korean Journal of Medicinal Crop Science. 19:54-65. https://doi.org/10.7783/KJMCS.2011.19.1.054
  19. Liu RH. (2004). Potential synergy of phytochemicals in cancer prevention : mechanism of action. The Journal of Nutrition. 134:3479S-3485S.
  20. Manach C, Williamson G, Morand C, Scalbert A and Remesy C. (2005). Bioavailability and bioefficacy of polyphenols in humans. The American Journal of Clinical Nutrition. 81:230S-242S.
  21. Marcocci L, Maguire JJ, Droy-Lefaix MT and Packer L. (1994). The nitric oxide-scavenging properties of Ginkgo biloba extract EGb 761. Biochemical and Biophysical Research Communications. 201:748-755. https://doi.org/10.1006/bbrc.1994.1764
  22. Marklund S and Marklund G. (1975). Involvement of superoxide anion radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase. European Journal of Biochemistry. 47:468-474.
  23. Moreno MIN, Isla MIN, Sampietro AR and Vattuone MA. (2000). Comparison of the free radical-scavenging activity of propolis from several region of Argentina. Journal of Ethnopharmacology. 71:109-114. https://doi.org/10.1016/S0378-8741(99)00189-0
  24. Oh YJ, Seo HR, Choi YM and Jung DS. (2010). Evaluation of antioxidant activity of the extracts from the aerial parts of Cnidium officinale Makino. Korean Journal of Medicinal Crop Science. 18:373-378.
  25. Radi R, Beckman JS, Bush KM and Freeman BA. (1991). Peroxynitrite oxidation of sulfhydryls, the cytotoxic potential of superoxide and nitric oxide. Journal of Biological Chemistry. 266:4244-4250.
  26. Ryu MJ, Lee SY, Park Y and Yang YK. (2010). Antioxidative activities and antifungal effect against Malassezia furfur in the extracts from 6 spp. medicinal plants. Journal of Korean Society of Cosmetology. 16:120-128.
  27. Seo YC, Choi WY, Kim JS, Zou YY, Lee CG, Ahn JH, Shin IS and Lee HY. (2010). Enhancement of antimicrobial activity of nano-encapsulated horseradish aqueous extracts against foodborne pathogens. Korean Journal of Medicinal Crop Science. 18:389-397.
  28. Shin KM, Park YM, Kim IT, Hong SP, Hong JP and Lee KT. (2003). In vitro antiinflammatory activity of amygdalin in murine macrophage Raw264.7 cells. Korean Journal of Pharmacognosy. 34:223-227.
  29. Song JH. (2009). Current status and future strategies of antimicrobial resistance. Korean Journal of Medicine. 77:143-151.
  30. Videla LA and Fermandez V. (1988). Biochemical aspects of cellular oxidative stress. Archivos De Biologia y Medicina Experimentales. 21:85-92.
  31. Xu ML, Hu JH, Wang L, Kim HS, Jin CW and Cho DH. (2010). Antioxidant and anti-diabetes activity of extracts from Machilus thunbergii S. et Z. Korean Journal of Medicinal Crop Science. 18:34-39.
  32. Yoon TJ. (2008). Effect of water extracts from root of Taraxacum officinale in innate and adaptive immune responses in mice. Korean Journal of Food and Nutrition. 21:275-282.

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  7. Nutritional Composition and Antioxidative Activity of Different Parts of Taraxacum coreanum according to Drying Methods vol.19, pp.4, 2013, https://doi.org/10.14373/JKDA.2013.19.4.389
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  9. Antioxidant Activity and Cytotoxicity of Ethanol Extracts from Different Parts of Taraxacum coreanum Nakai cultivated in South Korea vol.28, pp.4, 2015, https://doi.org/10.9799/ksfan.2015.28.4.594
  10. Nitric Oxide Production Inhibitory and Scavenging Activity and Tyrosinase Inhibitory Activity of Extracts from Taraxacum officinale and Taraxacum coreanum vol.19, pp.5, 2011, https://doi.org/10.7783/KJMCS.2011.19.5.362