한국환경보건학회지 (Journal of Environmental Health Sciences)

  • 제37권4호
  • /
  • Pages.279-288
  • /
  • 2011
  • /
  • 1738-4087(pISSN)
  • /
  • 2233-8616(eISSN)
한국환경보건학회 (Korean Society of Environmental Health)
권호 표지
DOI QR코드

DOI QR Code

국내 일부 초등학교 바닥먼지 내 화학적 및 생물학적 유해인자의 분석

Chemical and Microbiological Hazard Analysis of Floor-Settled Dust in Elementary School Classrooms in Korea

  • 김지영 (서울대학교 보건대학원 환경보건학과) ;
  • 정세영 (서울대학교 보건대학원 환경보건학과) ;
  • 김수정 (서울대학교 보건대학원 환경보건학과) ;
  • 김진아 (서울대학교 보건대학원 환경보건학과) ;
  • 시지연 (서울대학교 보건대학원 환경보건학과) ;
  • 조연우 (서울대학교 보건대학원 환경보건학과) ;
  • 조경덕 (서울대학교 보건대학원 환경보건학과) ;
  • 고광표 (서울대학교 보건대학원 환경보건학과)
  • Kim, Ji-Young (Department of Environmental Health, Graduate School of Public Health, Seoul National University) ;
  • Jung, Se-Young (Department of Environmental Health, Graduate School of Public Health, Seoul National University) ;
  • Kim, Su-Jung (Department of Environmental Health, Graduate School of Public Health, Seoul National University) ;
  • Kim, Jin-Ah (Department of Environmental Health, Graduate School of Public Health, Seoul National University) ;
  • Si, Ji-Yeon (Department of Environmental Health, Graduate School of Public Health, Seoul National University) ;
  • Jo, Yeon-Woo (Department of Environmental Health, Graduate School of Public Health, Seoul National University) ;
  • Zoh, Kyung-Duk (Department of Environmental Health, Graduate School of Public Health, Seoul National University) ;
  • Ko, Gwang-Pyo (Department of Environmental Health, Graduate School of Public Health, Seoul National University)
  • 투고 : 2011.03.21
  • 심사 : 2011.08.22
  • 발행 : 2011.08.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objectives: This study investigated the levels and components of floor-settled dust in two elementary schools located at different sites (one near the Shihwa industrial complex and the other in a rural area) in order to evaluate the amounts of trace metal elements (As, Cd, Co, Cr, Cu, Ni, Pb and Zn) and microorganisms. Methods: Over twenty settled-dust samples were collected from the two elementary schools. Trace metal elements were extracted from the dust using hydrochloric acid and nitric acid, and the amounts were measured by ICP-OES. Microbiological analysis was performed by bacterial culturing using R2A medium and denaturing gradient gel electrophoresis (DGGE). Results: The results showed that the amounts of three metal elements (Cr, Pb, and Zn) were significantly different between the schools (${\alpha}$=0.05, p<0.04). In addition, microbial communities in each school were highly correlated with one another. Among the identified microorganisms, a number of potentially opportunistic microorganisms, including antibiotic-resistant bacteria such as Acinetobacter baumannii, were found. Conclusions: This study will provide preliminary data for assessing levels and types of chemical and microbiological agents in elementary schools and for further evaluating human health risks associated with the agents.

키워드

참고문헌

  1. Perez-Padilla R, Schilmann A, Riojas-Rodriguez H. Respiratory health effects of indoor air pollution. Int J Tuberc Lung Dis. 2010; 14(9): 1079-1086.
  2. Heinrich J. Influence of indoor factors in dwellings on the development of childhood asthma. Int J Hyg Environ Health. 2011; 214(1): 1-25. https://doi.org/10.1016/j.ijheh.2010.08.009
  3. Weisel CP. Assessing exposure to air toxics relative to asthma. Environ Health Perspect. 2002; 110 Suppl 4; 527-537.
  4. Weisel CP, Zhang J, Turpin BJ, Morandi MT, Colome S, Stock TH, et al. Relationship of Indoor, Outdoor and Personal Air (RIOPA) study: study design, methods and quality assurance/control results. J Expo Anal Environ Epidemiol. 2005; 15(2): 123-137. https://doi.org/10.1038/sj.jea.7500379
  5. Park JH, Exposure Assessment of Biological Agents in indoor Environments, J Environ Health Sci. 2009; 35(4): 239-248. https://doi.org/10.5668/JEHS.2009.35.4.239
  6. Macher JM. Review of methods to collect settled dust and isolate culturable microorganisms. Indoor Air. 2001; 11(2): 99-110. https://doi.org/10.1034/j.1600-0668.2001.110204.x
  7. Song DU, Song CG, Kim CH. A Study on the Atmospheric Dispersion of Odor Emitted from Banwol/ Sihwa Industrial Complex in Ansan Area. Kor Soc Environ Impact Assess. 2003; 12(5): 323-340.
  8. Song HB, Do HS, Lee MS, Shin DC, Yoon HS, Kwak JH, et al. Trace Elements Analysis and Source Assessment of School Dust in Daegu, Korea. J Kor Soc of Environ Engineers. 2007; 29(12): 1390-1399.
  9. Do HS, Song HB, Jung YW, Yoon HS, Kwak JH, Han JU, et al. Trace Element Analysis and Source Assessment of Household Dust in Daegu, Korea. J Kor Soc of Environ Engineers. 2010; 32(1): 1237- 1246.
  10. Ordonez A, Loredo J, De Miguel E, Charlesworth S. Distribution of heavy metals in the street dusts and soils of an industrial city in northern Spain. Arch Environ Contam Toxicol. 2003; 44(2): 160- 170. https://doi.org/10.1007/s00244-002-2005-6
  11. United State Environmental Protection Agency. Environmental Monitoring Systems Handbook of Analytical Quality Control in Water and Wastewater Laboratories. 1979: EPA-600/4-79-019.
  12. Macher JM. Evaluation of a procedure to isolate culturable microorganisms from carpet dust. Indoor Air. 2001; 11(2): 134-140. https://doi.org/10.1034/j.1600-0668.2001.110208.x
  13. Roy SR, Schiltz AM, Marotta A, Shen Y, Liu AH. Bacterial DNA in house and farm barn dust. J Allergy Clin Immunol. 2003; 112(3): 571-578. https://doi.org/10.1016/S0091-6749(03)01863-3
  14. Muyzer G, de Waal EC, Uitterlinden AG. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl Environ Microbiol. 1993; 59(3): 695- 700.
  15. Webster G, Newberry CJ, Fry JC, Weightman AJ. Assessment of bacterial community structure in the deep sub-seafloor biosphere by 16S rDNA-based techniques: a cautionary tale. J Microbiol Methods. 2003; 55(1): 155-164. https://doi.org/10.1016/S0167-7012(03)00140-4
  16. Nicolaisen MH, Ramsing NB. Denaturing gradient gel electrophoresis (DGGE) approaches to study the diversity of ammonia-oxidizing bacteria. J Microbiol Methods. 2002; 50(2): 189-203. https://doi.org/10.1016/S0167-7012(02)00026-X
  17. Lee S, Choi B, Yi SM, Ko G. Characterization of microbial community during Asian dust events in Korea. Sci Total Environ. 2009; 407(20): 5308- 5314. https://doi.org/10.1016/j.scitotenv.2009.06.052
  18. Choi SW, Song HD. Source Characteristics of Particulate Trace Metals in Daegu Area. J Kor Soc for Atmosph Environ. 2000; 16(5): 469-476.
  19. Ferreia-Baptista L, Miguel E. Geochemistry and risk assessment of street dust in Luanda, Angola: A tropical urban environment. Atmospheric Environ. 2005; 39(25): 4501-4512. https://doi.org/10.1016/j.atmosenv.2005.03.026
  20. Tong ST, Lam KC. Home sweet home? A case study of household dust contamination in Hong Kong. Sci Total Environ. 2000; 256(2-3): 115-123. https://doi.org/10.1016/S0048-9697(00)00471-X
  21. Zhang R, Wang M, Kanai Y, Ohta A. Seasonal Characterization of Dust Days, Mass Concentration and Dry Deposition of Atmospheric Aerosols over Qingdao, China. China Particuology. 2004; 2(5): 196-199. https://doi.org/10.1016/S1672-2515(07)60058-X
  22. Douwes J, Thorne P, Pearce N, Heederik D. Bioaerosol health effects and exposure assessment: progress and prospects. Ann Occup Hyg. 2003; 47(3): 187-200. https://doi.org/10.1093/annhyg/meg032
  23. Shin DC, Lim YU, Yang JY, Kim HY. Study about prevention and correlation at indoor air quality in school and environmental disease, The institute for Environmental Research, Yonsei University College of Medicine; 2006.
  24. Rintala H, Pitkaranta M, Toivola M, Paulin L, Nevalainen A. Diversity and seasonal dynamics of bacterial community in indoor environment. BMC Microbiol. 2008; 8: 56.
  25. Bergogne-Berezin E, Towner KJ. Acinetobacter spp. as nosocomial pathogens: microbiological, clinical, and epidemiological features. Clin Microbiol Rev. 1996; 9(2): 148-165.
  26. CDC : http: //www.cdc.gov/HAI/organisms/acinetobacter.html/. [Accessed 20 January 2011].
  27. Smith MG, Gianoulis TA, Pukatzki S, Mekalanos JJ, Ornston LN, Gerstein M, et al. New insights into Acinetobacter baumannii pathogenesis revealed by high-density pyrosequencing and transposon mutagenesis. Genes Dev. 2007; 21(5): 601-614. https://doi.org/10.1101/gad.1510307
  28. Chen L, Yang L, Zhao X, Shen L, Duan K. Identification of Pseudomonas aeruginosa genes associated with antibiotic susceptibility. Sci China Life Sci. 2010; 53(10): 1247-1251. https://doi.org/10.1007/s11427-010-4071-8
  29. Tronel H, Chaudemanche H, Pechier N, Doutrelant L, Hoen B. Endocarditis due to Neisseria mucosa after tongue piercing. Clin Microbiol Infect. 2001; 7(5): 275-276. https://doi.org/10.1046/j.1469-0691.2001.00241.x
  30. Lee JH, Min BH. Distribution and characteristics of heavy metals in the fallout dust deposits at the middle and high schools of some cities in South Korea. Kor J Environ Health. 2003; 29(3): 21-27.
  31. Tong STY. Indoor and outdoor household dust contamination in Cincinnati, Ohio, USA. Environ Geochem and Health. 1998; 20(3): 123-133. https://doi.org/10.1023/A:1006561832381
  32. Chattopadhyay G, Lin KC, Feitz AJ. Household dust metal levels in the Sydney metropolitan area. Environ Res. 2003; 93(3): 301-307. https://doi.org/10.1016/S0013-9351(03)00058-6