대한수의학회지 (Korean Journal of Veterinary Research)

  • 제51권3호
  • /
  • Pages.249-252
  • /
  • 2011
  • /
  • 2466-1384(pISSN)
  • /
  • 2466-1392(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
권호 표지
DOI QR코드

DOI QR Code

An animal model using Eimeria live vaccine and to study coccidiosis protozoa pathogenesis

  • Lee, Hyun-A (Center for Animal Resources Development, Wonkwang University) ;
  • Hong, Sunhwa (Center for Animal Resources Development, Wonkwang University) ;
  • Choe, Ohmok (Center for Animal Resources Development, Wonkwang University) ;
  • Kim, Okjin (Center for Animal Resources Development, Wonkwang University)
  • 투고 : 2011.06.03
  • 심사 : 2011.09.22
  • 발행 : 2011.09.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Cell culture systems for the protozoan Eimeria are not yet available. The present study was conducted to develop an animal model system by inoculating animals with a live Eimeria vaccine. This study was conducted on 3-day-old chickens (n = 20) pretreated with cyclophosphamide. The chickens were divided into 2 groups: the control group (n = 10) and the inoculated group that received the live Eimeria vaccine (n = 10). During the study period, we compared the clinical signs, changes in body weight, and number of oocysts shed in the feces of the control and inoculated group. This study showed that oocyst shedding was significantly higher in the chickens inoculated with live Eimeria oocysts than in the control chickens. Moreover, body weight gain was lesser in the animals in the inoculated group than in the control animals. Fecal oocyst shedding was observed in the inoculated animals. On the basis of these findings, we suggest that live Eimeria vaccination with cyclophosphamide pretreatment may be used to obtain an effective animal model for studying protozoan infections. This animal study model may eliminate the need for a tedious continuous animal inoculation process every 6 months because the live coccidiosis vaccine contains live oocysts.

키워드

참고문헌

  1. Allen PC, Fetterer RH. Recent advances in biology and immunobiology of Eimeria species and in diagnosis and control of infection with these coccidian parasites of poultry. Clin Microbiol Rev 2002, 15, 58-65. https://doi.org/10.1128/CMR.15.1.58-65.2002
  2. Chapman HD, Matsler PL, Muthavarapu VK, Chapman ME. Acquisition of immunity to Eimeria maxima in newly hatched chickens given 100 oocysts. Avian Dis 2005, 49, 426-429. https://doi.org/10.1637/7359-032805R1.1
  3. Chaudhry KM. Immunomodulatory effects of coccidiostats in broiler chicks. Master thesis. University of Agriculture, Faisalabad, Pakistan, 1991.
  4. Cox FEG. Control of coccidiosis: lessons from other sporozoa. Int J Parasitol 1998, 28, 165-179. https://doi.org/10.1016/S0020-7519(97)00166-5
  5. Dalloul RA, Lillehoj HS. Recent advances in immunomodulation and vaccination strategies against coccidiosis. Avian Dis 2005, 49, 1-8. https://doi.org/10.1637/7306-11150R
  6. Kim Y, Brown TP, Pantin-Jackwood MJ. The effects of cyclophosphamide treatment on the pathogenesis of subgroup J avian leukosis virus (ALV-J) infection in broiler chickens with Marek's disease virus exposure. J Vet Sci 2004, 5, 49-58.
  7. Lillehoj HS, Trout JM. Avian gut-associated lymphoid tissues and intestinal immune responses to Eimeria parasites. Clin Microbiol Rev 1996, 9, 349-360.
  8. Markowski-Grimsrud CJ, Schat KA. Infection with chicken anaemia virus impairs the generation of pathogen-specific cytotoxic T lymphocytes. Immunology 2003, 109, 283-294. https://doi.org/10.1046/j.1365-2567.2003.01643.x
  9. McDougald LR. The growth of avian Eimeria in vitro. In: Long PL, Boorman KN, Freeman BM (eds.). Avian Coccidiosis. pp. 185-223, British Poultry Science, Edinburgh, 1978.
  10. Min W, Dalloul RA, Lillehoj HS. Application of biotechnological tools for coccidia vaccine development. J Vet Sci 2004, 5, 279-288.
  11. Min W, Lillehoj HS, Burnside J, Weining KC, Staeheli P, Zhu JJ. Adjuvant effects of IL-1${\beta}$, IL-2, IL-8, IL-15, IFN-${\alpha}$, IFN-${\gamma}$, TGF-4 and lymphotactin on DNA vaccination against Eimeria acervulina. Vaccine 2001, 20, 267-274. https://doi.org/10.1016/S0264-410X(01)00270-5
  12. Misra RR, Bloom SE. Roles of dosage, pharmacokinetics, and cellular sensitivity to damage in the selective toxicity of cyclophosphamide towards B and T cells in development. Toxicology 1991, 66, 239-256. https://doi.org/10.1016/0300-483X(91)90196-8
  13. Muruganandan S, Lal J, Gupta PK. Immunotherapeutic effects of mangiferin mediated by the inhibition of oxidative stress to activated lymphocytes, neutrophils and macrophages. Toxicology 2005, 215, 57-68. https://doi.org/10.1016/j.tox.2005.06.008
  14. Naeem K, Niazi T, Malik SA, Cheema AH. Immunosuppressive potential and pathogenicity of an avian adenovirus isolate involved in hydropericardium syndrome in broilers. Avian Dis 1995, 39, 723-728. https://doi.org/10.2307/1592408
  15. Sharma JM, Kim IJ, Rautenschlein S, Yeh HY. Infectious bursal disease virus of chickens: pathogenesis and immunosuppression. Dev Comp Immunol 2000, 24, 223-235. https://doi.org/10.1016/S0145-305X(99)00074-9
  16. Shirley MW. Eimeria species and strains of chicken. In: Eckert J, Braun R, Shirley MW, Coudert P (eds.). Guidelines on Techniques in Coccidiosis Research. pp. 1-25, European Commission, Luxemburg, 1995.
  17. Williams RB. Fifty years of anticoccidial vaccines for poultry (1952-2002). Avian Dis 2002, 46, 775-802. https://doi.org/10.1637/0005-2086(2002)046[0775:FYOAVF]2.0.CO;2