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Protective effects of a transgenic carrot vaccine on piglet diarrhea

자돈 설사병에 대한 형질전환 당근백신의 방어 효능 평가

  • Kim, Yeong-Hun (College of Veterinary Medicine and Institution of Veterinary Science, Kangwon National University) ;
  • Nam, Jin-Young (School of bioresource Sciences, Dankook University) ;
  • Lee, Hyang-Keun (School of bioresource Sciences, Dankook University) ;
  • Hwang, Cheol-Ho (School of bioresource Sciences, Dankook University) ;
  • Han, Jeong-Hee (College of Veterinary Medicine and Institution of Veterinary Science, Kangwon National University)
  • 김영훈 (강원대학교 수의과대학 및 동물의학종합연구소) ;
  • 남진영 (단국대학교 생명자원과학부) ;
  • 이향근 (단국대학교 생명자원과학부) ;
  • 황철호 (단국대학교 생명자원과학부) ;
  • 한정희 (강원대학교 수의과대학 및 동물의학종합연구소)
  • Accepted : 2011.03.14
  • Published : 2011.06.30

Abstract

The study evaluated whether a transgenic carrot vaccine could induce a K88-specific immune response in sows and whether the resultant maternal antibody could protect piglets against enterotoxigenic Escherichia coli (ETEC) K88ac infection. Sows (n = 15) selected randomly from a farm in Korea were assigned to three groups (n = 5 per group: control [untreated]), group A (orally inoculated with a nontransgenic and transgenic carrot vaccines at 2 and 4 weeks ante partum, respectively), and group B (conventionally vaccinated according to the manufacturer's instructions). After 7 days of lactation, 5 piglets selected randomly from each group were challenged with $1{\times}10^{10}$ colony forming units/mL ETEC K88ac. Group C had the lowest mean fecal consistency score on post-challenge days 1 and 7. Histiologically, On post-challenge day 7, group C showed an increased duodenum and ileum villus:crypt ratio, compared to group A in the duodenum, with group B displaying the highest ratio. Groups B and C had more increased villus width than group A in the jejunum. Group C displayed the greatest increase in villus width in the ileum. The colostrums and serum from groups B and C displayed higher concentrations of IgA and IgG against ETEC K88, compared to group A. Based on the results, it was concluded that the transgenic carrot vaccine in sow per oral may have an effect on preventing piglet diarrhea as good as commercial recombinant vaccine.

Keywords

References

  1. 이영선, 황철호. 자돈 설사병 방지를 위한 경구백신용 형질전환 당근 개발. 식물생명공학회지 2002, 29, 287-293.
  2. Austin S, Bingham ET, Koegel RG, Mathews DE, Shahan MN, Straub RJ, Burgess RR. An overview of a feasibility study for the production of industrial enzymes in transgenic alfalfa. Ann N Y Acad Sci 1994, 721, 234-244. https://doi.org/10.1111/j.1749-6632.1994.tb47395.x
  3. Brown J, Resurreccion RS, Dickson TG, Horne A. The relationship of egg yolk and serum antibody. I. Infectious bursal disease virus. Avian Dis 1989, 33, 654-656. https://doi.org/10.2307/1591140
  4. Chae C. Shiga toxin, Shiga-like toxin and Verotoxin. Korea J Vet Res 1993, 33, 773-779.
  5. Demicheli V, Jefferson T. Economic aspects of vaccination. Vaccine 1996, 14, 941-943. https://doi.org/10.1016/0264-410X(96)00088-6
  6. Fagarasan S, Kinoshita K, Muramatsu M, Ikuta K, Honjo T. In situ class switching and differentiation to IgA-producing cells in the gut lamina propria. Nature 2001, 413, 639-643. https://doi.org/10.1038/35098100
  7. Fairbrother JM, Gyles CL. Escherichia coli infections. In: Straw BE, Zimmerman JJ, D' Allaire S, Taylor DJ (eds.). Diseases of Swine. 9th ed. pp. 639-674, Blackwell, Ames, 2006.
  8. Fairbrother JM, Nadeau E, Gyles CL. Escherichia coli in postweaning diarrhea in pigs: an update on bacterial types, pathogenesis, and prevention strategies. Anim Health Res Rev 2005, 6, 17-39. https://doi.org/10.1079/AHR2005105
  9. Gaastra W, de Graaf FK. Host-specific fimbrial adhesins of noninvasive enterotoxigenic Escherichia coli strains. Microbiol Rev 1982, 46, 129-161.
  10. Gelberg HB. Alimentary system. In: McGavin MD, Zachary JF (eds.). Pathologic Basis of Veterinary Disease. 4th ed. pp. 361-363, Mosby, St. Louis, 2007.
  11. Hampson DJ, Smith WC. Influence of creep feeding and dietary intake after weaning on malabsorption and occurrence of diarrhoea in the newly weaned pig. Res Vet Sci 1986, 41, 63-69.
  12. Hooper BE, Haelterman EO. Concepts of pathogenesis and passive immunity in transmissible gastroenteritis of swine. J Am Vet Med Assoc 1966, 149, 1580-1586.
  13. Krugmann S, Pleass RJ, Atkin JD, Woof JM. Structural requirements for assembly of dimeric IgA probed by site-directed mutagenesis of J chain and a cysteine residue of the $\alpha$-chain CH2 domain. J immunol 1997, 159, 244-249.
  14. Levine MM. Escherichia coli that cause diarrhea: enterotoxigenic, enteropathogenic, enteroinvasive, enterohemorrhagic, and enteroadherent. J infect Dis 1987, 155, 377-389. https://doi.org/10.1093/infdis/155.3.377
  15. Levine MM, Kaper JB, Black RE, Clements ML. New knowledge on pathogenesis of bacterial enteric infections as applied to vaccine development. Microbiol Rev 1983, 47, 510-550.
  16. Macpherson AJ, Gatto D, Sainsbury E, Harriman GR, Hengartner H, Zinkernagel RM. A primitive T cell-independent mechanism of intestinal mucosal IgA responses to commensal bacteria. Science 2000, 288, 2222-2226. https://doi.org/10.1126/science.288.5474.2222
  17. Nataro JP, Kaper JB. Diarrheagenic Escherichia coli. Clin Microbiol Rev 1998, 11, 142-201.
  18. Rao MC. Toxins which activate guanylate cyclase: Heat-stable enterotoxins. Ciba Found Symp 1985, 112, 74-93.
  19. Russell MW, Kilian M. Biological activities of IgA. In: Mestecky J, Lamm ME, Strober W, Bienenstock J, NcGhee JR, Mayer L (eds.). Mucosal Immunology. 3th ed. pp. 267-289, Elsevier, Amsterdam, 2005.
  20. Salmon H, Berri M, Gerdts V, Meurens F. Humoral and cellular factors of maternal immunity in swine. Dev Comp Immunol 2009, 33, 384-393. https://doi.org/10.1016/j.dci.2008.07.007
  21. Shea KM. Antibiotic resistance: what is the impact of agricultural uses of antibiotics on children's health? Pediatrics 2003, 112, 253-258.
  22. Sherman DM, Acres SD, Sadowski PL, Springer JA, Bray B, Raybould TJ, Muscoplat CC. Protection of calves against fatal enteric colibacillosis by orally administered Escherichia coli K99-specific monoclonal antibody. Infect Immun 1983, 42, 653-658
  23. Streatfield SJ. Mucosal immunization using recombinant plant-based oral vaccines. Methods 2006, 38, 150-157. https://doi.org/10.1016/j.ymeth.2005.09.013
  24. Svendsen L, Crowley A, Ostergaard LH, Stodulski G, Hau J. Development and comparison of purification strategies for chicken antibodies from egg yolk. Lab Anim Sci 1995, 45, 89-93.
  25. Tacket CO, Mason HS. A review of oral vaccination with transgenic vegetables. Microbes Infect 1999, 1, 777-783. https://doi.org/10.1016/S1286-4579(99)80080-X
  26. Tizard IR. Veterinary Immunology: An Introduction. 7th ed. pp. 256-258, Elsevier Saunders, Philadelphia, 2004.
  27. Twyman RM, Schillberg S, Fischer R. Transgenic plants in the biopharmaceutical market. Expert Opin Emerg Drugs 2005, 10, 185-218. https://doi.org/10.1517/14728214.10.1.185
  28. Uzogara SG. The impact of genetic modification of human foods in the 21st century: a review. Biotechnol Adv 2000, 18, 179-206. https://doi.org/10.1016/S0734-9750(00)00033-1
  29. Whitelam GC, Cockburn B, Gandecha AR, Owen MR. Heterologous protein production in transgenic plants. Biotechnol Genet Eng Rev 1993, 11, 1-29. https://doi.org/10.1080/02648725.1993.10647896
  30. Woof JM, Kerr MA. The function of immunoglobulin A in immunity. J Pathol 2006, 208, 270-282. https://doi.org/10.1002/path.1877