Properties of Semen and Sperm Motility of Black Porgy Acanthopagrus schlegelii Acclimated in Freshwater

담수순화 감성돔 Acanthopagrus schlegelii의 정액특성 및 정자운동성

  • Jeong, Min- Hwan (Aquaculture Management Division, National Fisheries Research and Development Institute) ;
  • Chang, Young-Jin (Dept. of Marine Bio-materials and Aquaculture, Pukyong National University)
  • 정민환 (국립수산과학원 양식관리과) ;
  • 장영진 (부경대학교 해양바이오신소재학과)
  • Received : 2011.02.17
  • Accepted : 2011.04.04
  • Published : 2011.06.30

Abstract

The comparison of the chemical properties of semen of black porgy Acanthopagrus schlegelii long-term acclimated reared in freshwater (BFW) and seawater (BSW) with sperm activity of salinity and ion composition. The chemical properties of seminal plasma on BFW of the factors that most there was not significant difference in the BSW. However, osmolality in seminal plasma of BFW and BSW was $307.0{\pm}4.6$ and $337.3{\pm}10.1$ mOsm/kg, respectively, where BFW showed significant lower concentration in contrast to BSW. Salinity effect on sperm motility of BFW and BSW in 0 psu solution, no sperm motility was observed, whereas in 10 psu solution, both BFW and BSW sperms showed low motility and short time post sperm activation. However, diluted in 20 and 32 psu solutions, highest motility and long time post sperm activation were observed in BFW and BSW sperm. SAI of BFW and BSW varied in depend on the osmolality regardless of ion kind and it showed the highest value in the similar osmolality of artificial seawater (956 mOsm/kg). Accordingly, even in sperm released from BFW, factors initiating sperm motility are determined by osmolality.

장기간 담수순화 사육한 감성돔 Acanthopagrus schlegelii(BFW) 정액의 화학적 특성과 염분 및 이온조성에 따른정자활성을 해수사육 감성돔(BSW) 정액과 비교하였다. BFW 정장의 화학적 특성은 대부분의 요인에서 BSW 정장과 차이를 보이지 않았으나, 삼투질농도는 각각 $307.0{\pm}4.6$, $337.3{\pm}10.1$ mOsm/kg으로 차이를 보였다. 염분에 따른 BFW 및BSW의 정자운동성는 0 psu에서는 운동성이 관찰되지 않았으나, 10 psu에서 낮은 운동성과 짧은 정자 운동지속시간을 보였다. 그러나 20, 32 psu에서는 높은 운동성과 긴 정자 운동지속시간 보였다. BFW와 BSW 정자의 SAI는 이온의 종류와는 상관없이 삼투질농도에 의존하여 변화하였으며, 인공해수와 비슷한 농도에서 높았다. 결론적으로 장기간 담수에서 사육한 감성돔의 정자의 운동개시요인은 환경수의 삼투질농도가 좌우하는 것으로 판단된다.

Keywords

References

  1. Alavi SMH, Cosson J (2006) Sperm motility in fishes: (II) Effects of ions and osmolality. Cell Biol Int 30:1-14. https://doi.org/10.1016/j.cellbi.2005.06.004
  2. Alavi SMH, Cosson J, Karami M, Mojazi Amiri B, Akhoundzadeh MA (2004) Spermatozoa motility in the persian sturgeon, Acipenser persicus: effects of pH, dilution rate, ions and osmolality. Reproduction 128:819-828. https://doi.org/10.1530/rep.1.00244
  3. Alavi SMH, Psenicka M, Rodina M, Policar T, Linhart O (2008) Changes of sperm morphology, volume, density and motility and seminal plasma composition in Barbus barbus (Teleostei: Cyprinidae) during the reproductive season. Aquat Living Resour 21:75-80. https://doi.org/10.1051/alr:2008011
  4. Alavi SMH, Rodina M, Policar T, Linhart O (2009) Relationship between semen characteristics and body size in Barbus barbus L. (Teleostei: Cyprinidae) and effects of ions and osmolality on sperm motility. Comp Biochem Physiol A 153:430-437. https://doi.org/10.1016/j.cbpa.2009.04.001
  5. Billard R, Cosson J, Perchec G, Linhart O (1995) Biology of sperm and artificial reproduction in carp. Aquaculture 124:95-112.
  6. Chang YJ, Choi YH, Lim HK, Kho KH (1999a) Milt property and sperm motility of grey mullet (Mugil cephalus). J Kor Fish Soc 32:238-241.
  7. Chang YJ, Lim HK, Chang YJ, Kim HS, Huh HT (1999b) Physico-chemical properties and cold storage of river puffer (Takifugu obscurus) milt. J Kor Fish Soc 32:243-246.
  8. Chang YJ, Lim HK, Kho KH (1995) Properties of semen and sperm motility in black porgy Acanthopagrus schlegeli. J Aquacult 8:149-157.
  9. Cosson J (2004) The ionic and osmotic factors controlling motility of fish spermatozoa. Aquac Int 12:69-85. https://doi.org/10.1023/B:AQUI.0000017189.44263.bc
  10. Cruca DD (1969) Some chemical and physical characteristics of fish sperm. Trans Ame Fish Soc 4:785-788.
  11. De Kruger JC, Smit GL, Van Vuren JHJ, Ferreira JT (1984) Some chemical and physical characteristics of the semen of Cyprinus carpio L. and Oreochromis mossambicus (Peters). J Fish Biol 24:263-272. https://doi.org/10.1111/j.1095-8649.1984.tb04797.x
  12. Gwo JC (1995) Ultrastructural study of osmolality effect on sperm of three marine teleosts. In: Goetz FW, Thomas P. Eds., Reproductive Physiology of Fish 1995. Fish Symposium 1995, Austin pp119.
  13. Haddy JA, Pankhurst NW (2000) The effects of salinity on reproductive development, plasma steroid levels, fertilisation and egg survival in black bream Acanthopagrus butcheri. Aquaculture 112:363-377.
  14. Harris JH (1986) Reproduction of the Australian bass Macquaria noíemaculeata (Perciformes: Percichthyidae) in the Sydney basin. Aust J Mar Freshwater Res 37:209-235. https://doi.org/10.1071/MF9860209
  15. Hatef A, Niksirat H, Amiri BM, Alavi SMH, Karami M (2007) Sperm density, seminal plasma composition and their physiological relationship in the endangered caspian brown trout Salmo trutta caspius. Aquacult Res 38:1175-1181. https://doi.org/10.1111/j.1365-2109.2007.01784.x
  16. Kho KH (2007) Milt property and sperm motility of panther puffer, Takifugu pardalis. Kor J Ichthyol 19:168-172.
  17. Kho KH, Morisawa M, Choi KS (2003) Membrane hyperpolarization increases cAMP to induce the initiation of sperm motility in salmonid fishes, rainbow trout and masu salmon. J Micro Biotech 13:833-840.
  18. Kho KH, Morisawa M, Choi KS (2004) The role of $Ca^{2+}$ and calmodulin on the initiation of sperm motility in salmonid fishes. J Micro Biotech 14:456-465.
  19. Kho KH, Tanimoto S, Inaba K, Oka Y, Morisawa M (2001) Transmembrane cell signaling for the initiation of trout sperm motility: Roles of ion channels and membrane hyperpolarization for cyclie AMP synthesis. Zool Sci 18:919-928. https://doi.org/10.2108/zsj.18.919
  20. Krasznai Z, Marian T, Izumi H, Damjanovich S, Balkay L, Tron L, Morisawa M (2000) Membrane hyperpolarization removes inactivation of $Ca^{2+}$ channels leading to $Ca^{2+}$ influx and initiation of sperm motility in the common carp. Proc Natl Acad Sci 97:2052-2067. https://doi.org/10.1073/pnas.040558097
  21. Lahnsteiner F, Berger B, Weismann T, Patzner RA (1996) Motility of spermatozoa of Albumus alburnus (Cyprinidae) and is relationship to seminal plasma composition and sperm metabolism. Fish Physiol Biochem 15:167-179. https://doi.org/10.1007/BF01875596
  22. Le MH, Lim HK, Min BH, Kim SY, Chang YJ (2007) Milt properties and spermatozoa structure of filefish Thamnaconus modestus. Dev Reprod 11:227-233.
  23. Lim HK, Byun SG, Lee JH, Park SU, Kim YC, Han HK, Min BH, Lee BY (2007) Sexual maturity and reproductive cycle of starry flounder Platichthys stellatus cultured in indoor tank. J Aquacult 20:212-218.
  24. Litvak MK, Trippel EA (1998) Sperm motility patterns of atlantic cod (Gadus morhua) in relation to salinity: effects of ovarian fluid and egg presence. Can J Fish Aquat Sci 55:1871-1877. https://doi.org/10.1139/f98-093
  25. Min BH, Kim BK, Hur JW, Bang IC, Byun SK, Choi CY, Chang YJ (2003) Physiological responses during freshwater acclimation of seawater-cultured black porgy (Acanthopagrus schlegeli). Kor J Ichthyol 15:265-275.
  26. Morisawa M (1985) Initiation mechanism of sperm motility at spawning in teleosts. Zool Sci 2:605-615.
  27. Morisawa M, Suzuki K (1980) Osmolality and potassium ion: Their roles in initiation of sperm motility in teleosts. Science 210:1145-1146. https://doi.org/10.1126/science.7444445
  28. Morisawa M, Suzuki K, Shimizu H, Morisawa S, Yasuda K (1983) Effects of osmolarity and potassium on motility of spermatozoa from freshwater cyprinid fishes. J Exp Biol 107:95-103.
  29. Morita M, Okuno M, Susilo ES, Setyo BP, Martarini D, Harnadi L, Takemura A (2006) Changes in sperm motility in response to osmolality / $Ca^{2+}$ in three Indonesian fresh water teleosts: goby (Oxyeleotris marmorata), Java carp (Puntius javanicus), and catfish (Clarias batrachus). Comp Biochem Physiol A 143:361-367. https://doi.org/10.1016/j.cbpa.2005.12.020
  30. Palmer PJ, Hogan AE, Barlow CG (1994) Chilled storage of pikey bream Acanthopagrus berda sperm and activation in different salinities. Asian Fish Sci 7:35-40.
  31. Strussmann CA, Renard P, Ling H, Takashima F (1994) Motility of pejerrey Odontesthes bonariensis spermatozoa. Fish Sci 60:9-13. https://doi.org/10.2331/fishsci.60.9
  32. Suquet M, Billard R, Cosson J, Dorange G, Chauvaud L, Mugnir C, Fauvel C (1994) Sperm features in turbot (Scophthalmus maximus): a comparison with other freshwater and marine fish species. Aquat Living Resour 7:283-294. https://doi.org/10.1051/alr:1994031
  33. Thorogood J, Blackshaw A (1992) Factors affecting the activation, motility and cryopreservation of the spermatozoa of the yellowfin bream, Acanthopagrus australis (Gunther). Aquacult Fish Manage 23:337-344.