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The Antioxidative Effects of Rhododendron brachycarpum Extracts

만병초(Rhododendron brachycarpum) 추출물의 항산화 효과

  • Rhim, Tae-Jin (Department of Biotechnology in Division of Animal and Life Resources, Sangji University) ;
  • Choi, Moo-Young (Department of Food and Nutrition, Sangji University)
  • 임태진 (상지대학교 동물생명자원학부 생명공학) ;
  • 최무영 (상지대학교 식품영양학과)
  • Received : 2011.05.06
  • Accepted : 2011.07.21
  • Published : 2011.08.31

Abstract

The objective of this study was to investigate the antioxidative capacity of Rhododendron brachycarpum 95% ethanol extracts. Total antioxidant status was examined by total antioxidant capacity against ABTS radical reactions. Total antioxidant capacities of R. brachycarpum extract at the concentrations of 0.2 and 1 mg/mL were 0.33 and 2.26 mM Trolox equivalents, respectively. Superoxide scavenging activities of R. brachycarpum extract at the concentrations of 0.2 and 1 mg/mL were 45.0 and 77.0%, respectively. Oxygen radical absorbance capacities of R. brachycarpum extract at the concentrations of 5 and 100 ${\mu}g/mL$ were 40.88 and 131.00 ${\mu}M$ Trolox equivalents, respectively. Total phenolic contents of R. brachycarpum extract at the concentrations of 0.2 and 1 mg/mL were 0.37 and 1.25 mM gallic acid equivalents, respectively. R. brachycarpum extract at the concentration of 0.1 mg/mL inhibited 0.2 mM and 0.5 mM tert-butyl hydroperoxide induced cyototoxicity by 52.1 and 30.3%, respectively, in HepG2 cell culture system. Thus, strong antioxidant and cytotoxicity-inhibiting effects of R. brachycarpum extract seem to be due to, at least in part, the prevention from free radicals-induced oxidation as well as high levels in total phenolic contents.

본 연구에서는 만병초 95% 에탄올추출물의 항산화 효과를 조사하였다. 총항산화 활성은 ABTS 라디칼에 대한 항산화능으로 측정하였다. 만병초 추출물 0.2 및 1 mg/mL의 총 항산화능은 각각 0.33 및 2.26 mM Trolox와 동등한 수준이었다. 만병초 추출물 0.2 및 1 mg/mL의 superoxide 소거능은 각각 45.0 및 77.0%이었다. 만병초 추출물 5 및 100 ${\mu}g/mL$의 산소라디칼 소거능은 각각 40.88 및 131.00 ${\mu}M$ Trolox와 동등한 수준이었다. 만병초 추출물 0.2 및 1 mg/mL의 총페놀 함량은 각각 0.37 및 1.25 mM gallic acid와 동등한 수준이었다. 또한, HepG2 세포주를 이용한 세포배양에서 만병초 추출물 0.1 mg/mL 농도의 첨가는 0.2 및 0.5 mM tert-butyl hydroperoxide로 유도된 세포독성을 각각 52.1 및 30.3% 감소시켰다. 따라서, 본 연구 결과들은 만병초 추출물의 강력한 항산화 효과와 세포독성 억제 효과를 나타내며, 이러한 효능은 적어도 자유라디칼의 산화 억제와 높은 총페놀 함량에 기인하는 것으로 사료된다.

Keywords

References

  1. Ahn, D.K. 2000. Illustrated book of Korean medicinal herbs. Kyohaksa. Seoul, Korea. p.751 (in Korean).
  2. Byun, K.S., Y.W. Lee, H.J. Jin, M.K., Lee, H.Y. Lee, K.J. Lee, M.Y. H, C.Y. Yu and J.H. Lee. 2005. Genotoxicity and cytotoxicity in human cancer and normal cell lines of the extracts of Rhododendron brachycarpum D. Don leaves. Korean J. Medicinal Crop Sci. 13:199-205 (in Korean).
  3. Choi, J.S., H.S. Young, J.C. Park, J.-H. Choi and W.S. Woo. 1986. Flavonoids from the leaves of Rhododendron brachycarpum. Arch. Pharm. Res. 9:233-36. https://doi.org/10.1007/BF02856638
  4. Erel, O. 2004. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin. Biochem. 37:277-285. https://doi.org/10.1016/j.clinbiochem.2003.11.015
  5. Halliwell, B., J.M.C. Gutteridge and C.E. Cross. 1992. Free radicals, antioxidants and human disease:where are we now? J. Lab Clin. Med. 119:598-620.
  6. Huang, D., B. Ou, M. Hampsch-Woodill, J. A. Flanagan and R. L. Prior. 2002. High-throughput assay of oxygen radical absorbance capacity (ORAC) using a multichannel liquid handling system coupled with a microplate fluorescence reader in 96-well format. J. Agric. Food Chem. 50:4437-4444. https://doi.org/10.1021/jf0201529
  7. Jung, S.J., D.-H. Kim, Y.-H. Hong, J.-H. Lee, H.-N. Song, Y.-D. Rho and N.-I. Baek. 2007. Flavonoids from the flower of Rhododendron yedoense var. Poukhanense and their antioxidant activities. Arch. Pharm. Res. 30:146-150. https://doi.org/10.1007/BF02977686
  8. Liu, F., V.E.C. Ooi and S.T. Chang. 1997. Free radical scavenging activities of mushroom polysaccharide extracts. Life Sci. 60:763-771. https://doi.org/10.1016/S0024-3205(97)00004-0
  9. Singleton, V.L. and R. Orthofer. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol. 299:152-178. https://doi.org/10.1016/S0076-6879(99)99017-1
  10. Steel, R.G.D. and J.H. Torrie. 1980. Principles and procedures of statistics, 2nd ed, McGraw-Hill, New York, pp. 186-187.
  11. Thannickal, V.J. and B.L. Fanburg. 2000. Reactive oxygen species in cell signaling. American J. Physiol. Lung Cell Mol. Physiol. 279:L1005-L1029.
  12. Tirmenstein, M.A., F.A. Nicholls-Grzemski, J.-G. Zhang and M.W. Fariss. 2000. Glutathione depletion and the production of reactive oxygen species in isolated hepatocyte suspensions. Chem. Biol. Interact. 127:201-217. https://doi.org/10.1016/S0009-2797(00)00180-0
  13. Yang Y.J., H.-J. Kim, S.-H. Kang and S.C. Kang. 2011. Screening of natural herb resources for anti-oxidative effects in Korea. Korean J. Plant Res. 24:1-9 (in Korean). https://doi.org/10.7732/kjpr.2011.24.1.001
  14. Youn, H. and J.-H. Cho. 1991. Isolation and structure elucidation of triterpenoidal constituents from the leaves of Rhododendron brachycarpum. Korean J. Pharmacogn. 22:18-21.

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