The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Comparison of Viability in Basidiomycetes After Low Temperature Storage According to Storage Period

저온 보존 기간에 따른 담자균류의 생존율 비교

  • Ryu, Sung-Ryul (Korea Forest Seed & Variety Center) ;
  • Ka, Kang-Hyeon (Department of Forest Resources Utilization, Korea Forest Research Institute) ;
  • Lee, Bong-Hun (Department of Forest Resources Utilization, Korea Forest Research Institute) ;
  • Park, Hyun (Department of Forest Resources Utilization, Korea Forest Research Institute) ;
  • Bak, Won-Chull (Department of Forest Resources Utilization, Korea Forest Research Institute)
  • 유성열 (국립산림품종관리센터) ;
  • 가강현 (국립산림과학원 녹색자원이용부) ;
  • 이봉훈 (국립산림과학원 녹색자원이용부) ;
  • 박현 (국립산림과학원 녹색자원이용부) ;
  • 박원철 (국립산림과학원 녹색자원이용부)
  • Received : 2011.06.13
  • Accepted : 2011.07.27
  • Published : 2011.08.01
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Abstract

Short-preservation of basidiomycetes is generally being conducted in slant tubes containing solid medium based on agar. In this study, we investigated the vitality of 28 species and 76 strains preserved on potato dextrose agar (PDA) at $4^{\circ}C$ for 2~7 years. The survival rates of the fungi were 82%, 86%, 94%, 96%, 94%, and 94% for seven, six, five, four, three, and two years old preservation, respectively. The volume of medium in Lentinula edodes showed decrease after 2 years preserved. The pH of preserved medium was 5.42 in 2007 (two years old), but it became nearly neutral as increasing preservation term.

균류의 단기 보존은 일반적으로 한천이 포함된 고체 사면배지에서 이루어진다. 본 연구에서는 2~7년간 PDA 배지를 이용하여 $4^{\circ}C$에서 보관된 담자균류 28종 76개 균주들에 대한 활력을 조사하였다. 균의 생존율은 보존기간이 7년 82%, 6년 86%, 5년 94%, 4년 96%, 3년 94%, 2년 94%로 나타났다. 표고 균주의 배지 부피는 2년 이후부터 감소하였다. 보존된 배지의 pH는 2007년에 5.42였지만 보존기간이 길어질수록 거의 중성 pH에 도달하였다.

Keywords

References

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Cited by

  1. Effects of Preservation Period at Low Temperature on the Mycelial Growth and the Lignocellulolytic Enzyme Activities of Basidiomycetes vol.42, pp.4, 2014, https://doi.org/10.4489/KJM.2014.42.4.322