한국식품영양학회지 (The Korean Journal of Food And Nutrition)

  • 제24권4호
  • /
  • Pages.720-724
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  • 2011
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  • 1225-4339(pISSN)
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  • 2287-4992(eISSN)
한국식품영양학회 (The Korean Society of Food and Nutrition)
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탄닌분해효소를 이용한 녹차 농축액의 품질 변화

Changes in the Quality of Green Tea Concentration through Tannase Treatment

  • 김동호 (서원대학교 보은황토대추사업단) ;
  • 이진 (천안연암대학 외식산업과) ;
  • 강병선 (천안연암대학 친환경원예과)
  • Kim, Dong-Ho (Institute of Industry-Academy Collaboration, Seowon University) ;
  • Lee, Jin (Dept. of Food Service Industry, Cheonan Yonam College) ;
  • Kang, Byung-Sun (Dept. of Eco-Friendly Horticulture, Cheonan Yonam College)
  • 투고 : 2011.11.15
  • 심사 : 2011.12.20
  • 발행 : 2011.12.31
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초록

녹차 농축액을 탄닌 가수분해 효소를 이용하여 가수 분해한 후 기존 녹차 농축액과 비교하여 이화학 및 관능적 검사를 실시하였다. pH, 항산화 능력은 효소분해 전후 유의적인 차이를 나타내지 않았으나, 효소 분해 후 탁도는 유의적으로 더욱 맑아졌다. 녹차 탄닌의 주성분인 카테킨류는 탄닌분해 효소에 의해 분해되어 전체적으로 감소하는 경향을 나타내었다. 갈레이트형 카테킨 성분인 에피갈로카테킨 갈레이트(EGCG)와 에피카테킨 갈레이트(ECG)는 EGC와 EC 그리고 gallic acid로 효소 분해되어 효소처리 녹차 농축액에서는 EGCG와 ECG가 측정되지 않았다. 떫은맛의 관능검사는 효소분해 후 유의적으로 떫은맛의 감소가 발생하였다. 따라서 탄닌분해 효소를 이용하여 녹차 추출물을 분해하면 녹차액은 맑아지지만, 기존 녹차 추출물의 문제점인 떫은맛은 감소되어 다양한 식품에 응용할 수 있는 것으로 나타났다.

Differences in sensory evaluation, physicochemical properties and antioxidant activities between green tea concentrations and tannase-treated green tea concentrations were measured in this study. The results showed that pH did not differ dependent on tannase treatment but antioxidant ability were slightly increased through tannase treatment without significant($p$ <0.05). However, the turbidity of the tannase-treated green tea concentration had significant difference to the non-treated concentration. The astringency, tested via sensory evaluation, decreased significantly after tannase treatment($p$ <0.05). Traces of epicatechin gallate(ECG) and epigallocatechin gallate(EGCG) also disappeared after the tannase treatment, due to the fact that the ester linkages in the catechin structure were broken by the tannase. It was concluded that tannase treatment of tea products is a very effective process for decreasing astringency and turbidity.

키워드

참고문헌

  1. Bajpai B, Patil S. 1997. Induction of tannin acyl hydrolase (EC 3.1.1.20) activity in some members of fungi imperfecti. Enzyme & Microbial Tech 20:612-614 https://doi.org/10.1016/S0141-0229(96)00206-2
  2. Bang BH, Park HH. 2000. Preparation of yogurt added with green tea and mugwort tea and quality characteristics. J Korean Sco Food Sci Nutr 29:854-859
  3. Battestin V, Macedo GA, De Freitas VAP. 2008. Hydrolysis of epigallocatechin gallate using a tannase from Paecilomyces variotii. Food Chem 108:228-233 https://doi.org/10.1016/j.foodchem.2007.10.068
  4. Boadi1 DK, Neufeld RJ. 2001. Encapsulation of tannase for the hydrolysis of tea tannins. Enzyme & Microbial Tech 28:590- 595 https://doi.org/10.1016/S0141-0229(01)00295-2
  5. Chandini SK, Rao LR, Gowthaman MK, Haware DJ, Subramanian R. 2011. Enzymatic treatment to improve the quality of black tea extracts. Food Chem 127:1039-1045 https://doi.org/10.1016/j.foodchem.2011.01.078
  6. Choi SH. 2009. Changes in the composition of catechins, theaflavins and alkaloids in leaves from Korean yabukida tea plant during processing to fermented black tea. Korean J Food Culture 24:308-314
  7. Choi Y, Lee SM, Hwang IG, Jeong HS, Lee J. 2011. Changes in antioxidant activity of duck egg after pressurized soaking in green tea extract. J Korean Soc Food Sci Nutr 40:1328- 1332 https://doi.org/10.3746/jkfn.2011.40.9.1328
  8. Chun JU. 2010. Rapid measure of color and catechins contents in processed teas using NIRS. Korean J Plant Res 23: 386- 392
  9. Chung YH, Shin MK. 2005. A study on the physicochemical properties of Korean teas according to degree of fermentation. Korean J Food & Nutr 18:94-101
  10. Fernandez-Lorente G, Bolivar JM, Rocha-Martin J, Curiel JA, Munoz R, de las Rivas B, Carrascosa AV, Guisan JM. 2011. Synthesis of propyl gallate by transesterification of tannic acid in aqueous media catalysed by immobilised derivatives of tannase from Lactobacillus plantarum. Food Chem 128: 214-217 https://doi.org/10.1016/j.foodchem.2011.02.057
  11. Gu LW, Kelm MA, Hammerstone JF, Beecher G, Holden J, Haytowitz D, Prior RL. 2003. Screening of foods containing proanthocyanidins and their structural characterization using LC-MS/MS and thiolytic degradation. J Agri & Food Chem 51:7513-7521 https://doi.org/10.1021/jf034815d
  12. Macedo JA, Battestin V, Ribeiro ML, Macedo GA. 2011. Increasing the antioxidant power of tea extracts by biotransformation of polyphenols. Food Chem 126:491-497 https://doi.org/10.1016/j.foodchem.2010.11.026
  13. Park KR, Lee SG, Nam TG, Kim YJ, Kim YR, Kim DO. 2009. Comparative analysis of catechins and antioxidant capacity in various grades of organic green teas grown in Boseong. Korean J Food Sci Technol 41:82-86
  14. Ravichandran R, Parthiban R. 1998. Changes in enzyme activities (polyphenol oxidase and phenylalanine ammonia lyase) with type of tea leaf and during black tea manufacture and the effect of enzyme supplementation of dhool on black tea quality. Food Chem 62:277-281 https://doi.org/10.1016/S0308-8146(97)00220-3
  15. Rodrıguez H, Rivas B, Gomez-Cordoves C, Munoz R. 2008. Degradation of tannic acid by cell-free extracts of Lactobacillus plantarum. Food Chem 107:664-670 https://doi.org/10.1016/j.foodchem.2007.08.063
  16. Singh S, Gupta R. 2004. Apple juice clarification using fungal pectinolytic enzyme and gelatin. Indian J of Biotech 3: 573-574
  17. Song KJ, Beak DC, Kim YW, Kim YG, Lee MS, Lee SP, Kim CS. 2010. Catechin and caffeine concentration variations in Jeju green tea varieties harvested over a seven-month period. J Food Sci Nutr 15:229-232 https://doi.org/10.3746/jfn.2010.15.3.229
  18. Sung NY, Kweon SY, Park JN, Choi JI, Song BS, Kim, JK, Lee JW, Kim JH. 2011. Effect of Yukwa containing green tea powder on lipid composition and body weight change in mice. J Korean Soc Food Sci Nutr 40:17-182
  19. Wee JH, Moon JH, Park KH. 1999. Catechin content and composition of domestic tea leaves at different plucking time. Korean J Food Sci Technol 31:20-23

피인용 문헌

  1. 버섯균사체 배양액 처리에 의한 아로니아 균질액의 탄닌 감소 및 이화학성 변화 vol.33, pp.3, 2011, https://doi.org/10.9799/ksfan.2020.33.3.339