Parasites, Hosts and Diseases

  • 제49권2호
  • /
  • Pages.133-138
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  • 2011
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  • 2982-5164(pISSN)
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  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
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Hematological and Serum Biochemical Analyses in Experimental Caprine Besnoitiosis

  • Nazifi, Saeed (Department of Clinical Studies, School of Veterinary Medicine, Shiraz University, Stem Cell and Transgenic Technology Research, Shiraz University) ;
  • Oryan, Ahmad (Department of Pathobiology, School of Veterinary Medicine, Shiraz University) ;
  • Namazi, Fatemeh (Department of Pathobiology, School of Veterinary Medicine, Shiraz University)
  • 투고 : 2011.03.17
  • 심사 : 2011.04.22
  • 발행 : 2011.06.30
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초록

This study was undertaken to investigate the hematological and biochemical changes in experimentally infected goats with Besnoitia caprae from the time of infection till 360 days post-infection (PI). Six male goats were inoculated subcutaneously with $13{\times}10^7$ bradyzoites of B. caprae, and blood samples were collected from the jugular vein. The total erythrocyte and total leukocyte counts, hematocrit value, and differential leukocyte counts were determined. Serum biochemical analysis, including the total protein, albumin, total globulin, cholesterol, triglyceride, chloride, testosterone, calcium ($Ca^{2+}$), inorganic phosphorus, sodium ($Na^+$), potassium ($K^+$), iron ($Fe^{2+}$), glucose, serum amyloid A (SAA), haptoglobin (Hp), fibrinogen, ceruloplasmin, aspartate aminotransferase, alanine aminotransferase, creatine kinase, lactate dehydrogenase, and alkaline phosphatase, was undertaken. Skin biopsy from the limbs were collected at weekly intervals and histologically examined for Besnoitia cysts. Cysts were present in the skin biopsies of the leg of the infected goats from day 28 PI. There were variations in hematological analyses, but no significant difference was seen. From day 30 to 360 PI, results showed that SAA, Hp, fibrinogen, and ceruloplasmin concentrations increased, whereas testosterone concentrations decreased. Infected goats exhibited decrease of albumin and increase of serum total protein and globulin concentrations. By contrast, there were no significant differences in the remained analyses concentrations.

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참고문헌

  1. Cheema AH, Toofanian F. Besnoitiosis in wild and domestic goats in Iran. Cornell Vet 1979; 69: 159-168.
  2. Bwangamoi O, Carles AB, Wandera JG. An epidemic of besnoitiosis in goats in Kenya. Vet Rec 1989; 125: 461. https://doi.org/10.1136/vr.125.18.461
  3. Dubey JP, Sreekumar C, Lindsay DS, Hill D, Rosenthal BM, Venturini L, Venturini MC, Greiner EC. Besnoitia oryctofelisi n. sp. (Protozoa: Apicomplexa) from domestic rabbits. Parasitology 2003; 126: 521-539.
  4. Eleni C, Crotti S, Manuali E, Costarelli S, Filippini G, Moscati L, Magnino S. Detection of Neospora caninum in an aborted goat fetus. Vet Parasitol 2004; 123: 271-274. https://doi.org/10.1016/j.vetpar.2004.06.017
  5. Ellis JT, Holmdahl OJM, Ryce C, Njenga JM, Harper PAW, Morrison DA. Molecular phylogeny of Besnoitia and the genetic relationships among Besnoitia of cattle, wildebeest and goats. Protist 2000; 151: 329-336. https://doi.org/10.1078/S1434-4610(04)70031-0
  6. Leighton FA, Gajadhar AA. Besnoitia spp. and besnoitiosis. In Samuel B, Pybus M, Kocan AM, eds, Parasitic Diseases of Wild Animals. Iowa State University Press. Iowa, USA. 2001, p 468-478.
  7. Oryan A, Sadeghi MJ. An epizootic of besnoitiosis in goats in Fars province of Iran. Vet Res Commun 1997; 21: 559-570. https://doi.org/10.1023/A:1005918931296
  8. Bwangamoi O, Njenga JM. A review of besnoitiosis with special reference to goats. Zimbabwe Vet J 1993; 24: 23-27.
  9. Oryan A, Azizi S. Ultrastructure and pathology of Besnoitia caprae in the naturally infected goats of Kerman, East of Iran. Parasitol Res 2008; 102: 1171-1176. https://doi.org/10.1007/s00436-008-0890-y
  10. Oryan A, Kafi M, Morgan-Azghadi N. Besnoitiosis of the reproductive tract of male goats. Comp Clin Path 2008; 17: 185-191. https://doi.org/10.1007/s00580-007-0717-8
  11. Nazifi S, Oryan A, Mohebbi H. Evaluation of hematological parameters in caprine besnoitiosis. J Appl Anim Res 2002; 21: 123-128. https://doi.org/10.1080/09712119.2002.9706364
  12. Oryan A, Nazifi S, Mohebbi H. Pathology and serum biochemical changes in natural caprine besnoitiosis. Rev Med Vet (Toulouse) 2008; 159: 27-32.
  13. Jain NC. Schalm's Veterinary Hematology, 4th ed. Philadelphia, USA. Lea & Febiger. 1986, p 20-80.
  14. McGowan MW, Artiss JD, Strandbergh DR, Zak B. A peroxidase-coupled method for the colorimetric determination of serum triglycerides. Clin Chem 1983; 29: 538-542.
  15. Burtis CA, Ashwood ER. Textbook of Clinical Chemistry, 2nd ed. Philadelphia, USA. Saunders. 1994, p 735-888.
  16. Sunderman FW Jr, Nomoto S. Measurement of human serum ceruloplasmin by its p-phenylenediamine oxidase activity. Clin Chem 1970; 16: 903-910.
  17. Thrall M, Baker AD, Lassen E, Campbell DT, Denicola D, Fettman M, Alan R, Weiser G. Veterinary Hematology and Clinical Chemistry. Philadelphia, USA. Lippincott Williams and Wilkins. 2004, p 355-377.
  18. Latimer KS, Mahaffey EA, Prasse KW. Veterinary Laboratory Medicine. Clinical Pathology. 4th ed. Iowa, USA. Iowa State Press. 2003, p 162-215.
  19. Kaneko JJ. Serum proteins and dysproteinaemias. In Kaneko JJ, Harvey JW, Bruss ML, eds, Clinical Biochemistry of Domestic Animals, 5th ed. San Diego, USA. Academic Press. 1997, p 117-138.
  20. Jain NC. Essentials of Veterinary Hematology, 1st ed. Philadelphia, USA. Lea & Febiger. 1993, p 295-306.
  21. Stockham SL, Scott MA. Fundamentals of Veterinary Clinical Pathology. 2nd ed. Iowa, USA. 2008, p 251-277 and 433-461.
  22. Eckersall PD. The time is right for acute phase protein assays. Vet J 2004; 168: 3-5. https://doi.org/10.1016/S1090-0233(03)00165-5
  23. Murata H, Shimada N, Yoshioka M. Current research on acute phase proteins in veterinary diagnosis: an overview. Vet J 2004; 168: 28-40. https://doi.org/10.1016/S1090-0233(03)00119-9
  24. Gruys E, Toussaint MJM, Niewold TA, Koopmans SJ. Acute phase reaction and acute phase proteins. J Zhejiang Univ Sci 2005; 6: 1045-1056.
  25. Eckersall PD. Recent advances and future prospects for the use of acute phase proteins as markers of disease in animals. Rev Med Vet (Toulouse) 2000; 151: 577-584.
  26. Murata H. Stress and acute phase protein response: an inconspicuous but essential linkage. Vet J 2007; 173: 473-474. https://doi.org/10.1016/j.tvjl.2006.05.008
  27. Feldman BF, Zinkl JG, Jain NC. Schalm's Veterinary Hematology. Philadelphia, USA. Lippincott Williams & Wilkins. 2000, p 891-896.
  28. Alsemgeest SPM, Lambooy IE, Wierenga HK, Dieleman SJ, Meerkerk B, van Ederen AM, Niewold TA. Influence of physical stress on the plasma concentrations of serum amyloid A (SAA) and haptoglobin (Hp) in calves. Vet Q 1995; 17: 9-12.
  29. Hicks TA, McGlone JJ, Whisnant CS, Kattesh HG, Norman RL. Behavioral, endocrine, immune, and performance measures for pigs exposed to acute stress. J Anim Sci 1998; 76: 474-483. https://doi.org/10.2527/1998.762474x
  30. Arthington JD, Eicher SD, Kunkle WE, Martin FG. Effect of transportation and commingling on the acute-phase protein response, growth, and feed intake of newly weaned beef calves. J Anim Sci 2003; 81: 1120-1125. https://doi.org/10.2527/2003.8151120x
  31. Hickey MC, Drennan M, Earley B. The effect of abrupt weaning of suckler calves on the plasma concentrations of cortisol, catecholamines, leukocytes, acute phase proteins and in vitro interferon gamma production. J Anim Sci 2003; 81: 2847-2855. https://doi.org/10.2527/2003.81112847x
  32. Pineiro M, Pineiro C, Carpintero R, Morales J, Campbell FM, Eckersall PD, Toussaint MJ, Lampreave F. Characterisation of the pig acute phase protein response to road transport. Vet J 2007; 173: 669-674. https://doi.org/10.1016/j.tvjl.2006.02.006
  33. Alsemgeest SPM, Kalsbeek HC, Wensing T, Koeman JP, van Ederen AM, Gruys E. Concentrations of serum amyloid A (SAA) and haptoglobin (Hp) as parameters of inflammatory diseases in cattle. Vet Q 1994; 16: 21-23. https://doi.org/10.1080/01652176.1994.9694410
  34. Katoh N, Nakagawa H. Detection of haptoglobin in the high-density lipoprotein and the very high-density lipoprotein fractions from sera of calves with experimental pneumonia and cows with naturally occurring fatty liver. J Vet Med Sci 1999; 61: 119-124. https://doi.org/10.1292/jvms.61.119
  35. Ganheim C, Hulten C, Carlsson U, Kindahl H, Niskanen R, Waller KP. The acute phase response in calves experimentally infected with bovine viral diarrhea virus and/or Mannheimia haemolytica. J Vet Med B Infect Dis Vet Public Health 2003; 50: 183-190. https://doi.org/10.1046/j.1439-0450.2003.00658.x
  36. Uhlar CM, Whitehead AS. Serum amyloid A, the major vertebrate acute-phase reactant. Eur J Biochem 1999; 265: 501-523. https://doi.org/10.1046/j.1432-1327.1999.00657.x
  37. Glass EJ, Graigmile SC, Springbett A, Preston PM, Kirvar E, Wilkie GM, Eckersall PD, Hall FR, Brown CG. The protozoan parasite Theileria annulata induces a distinct acute phase protein response in cattle that is associated with pathology. Int J Parasitol 2003; 33: 1409-1418. https://doi.org/10.1016/S0020-7519(03)00166-8
  38. Gentry PA. Comparative aspects of blood coagulation. Vet J 2004; 168: 238-251. https://doi.org/10.1016/j.tvjl.2003.09.013
  39. Kafi M, Oryan A, Morgan-Azghadi N. Pathology of testis and epididymis in native goats in southern Iran. Comp Clin Path 2007; 16: 201-205. https://doi.org/10.1007/s00580-006-0662-y
  40. Njenga MJ, Munyua SJ, Mutiga ER, Gathuma JM, Kang'ethe EK, Bwangamoi O, Mugera GM, Mitaru BN. Semen characteristics of goats with subacute, acute and chromic besnoitiosis. J S Afr Vet Assoc 1999; 70: 18-20.

피인용 문헌

  1. Natural Besnoitia besnoiti infections in cattle: hematological alterations and changes in serum chemistry and enzyme activities vol.11, pp.None, 2011, https://doi.org/10.1186/s12917-015-0326-8
  2. Effect of Ketoprofen on acute phase protein concentrations in goats undergoing castration vol.12, pp.None, 2016, https://doi.org/10.1186/s12917-016-0748-y