BMB Reports

생화학분자생물학회 (Korean Society for Biochemistry and Molecular Biology)
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Estrogen receptor β stimulates Egr-1 transcription via MEK1/Erk/Elk-1 cascade in C6 glioma cells

  • Kim, Ji-Ha (Division of Molecular and Life Sciences, College of Science and Technology, Hanyang University) ;
  • Jeong, Il-Yeup (Division of Molecular and Life Sciences, College of Science and Technology, Hanyang University) ;
  • Lim, Yoong-Ho (Division of Bioscience and Biotechnology, BMIC) ;
  • Lee, Young-Han (Department of Biomedical Science and Technology, SMART Institute of Advanced Biomedical Science) ;
  • Shin, Soon-Young (Department of Biomedical Science and Technology, SMART Institute of Advanced Biomedical Science)
  • 투고 : 2011.03.11
  • 심사 : 2011.04.27
  • 발행 : 2011.07.31
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초록

The Egr-1 is an immediate early response gene encoding a transcription factor that functions in the regulation of cell growth, differentiation, and apoptosis. Estrogen has diverse physiological effects, including cellular proliferation and neuroprotection against brain injury. There are two types of estrogen receptors (ERs), $ER{\alpha}$ and $ER{\beta}$. $ER{\alpha}$-induced Egr-1 expression has been extensively studied; however, the role of $ER{\beta}$ is yet not known. In the present study, we investigated whether or not $ER{\beta}$ induces Egr-1 expression in C6 rat glioma cells, which express $ER{\beta}$ but not $ER{\alpha}$. Our results show that $ER{\beta}$ promoted up-regulation of Egr-1 expression via a non-genomic mechanism involving the Raf/MEK1/Erk/Elk-1 signaling cascade.

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참고문헌

  1. McEwen, B. S. and Alves, S. E. (1999) Estrogen actions in the central nervous system. Endocr. Rev. 20, 279-307. https://doi.org/10.1210/er.20.3.279
  2. Czlonkowska, A., Ciesielska, A. and Joniec, I. (2003) Influence of estrogens on neurodegenerative processes. Med. Sci. Monit. 9, RA247-256.
  3. Mosselman, S., Polman, J. and Dijkema, R. (1996) ER beta: identification and characterization of a novel human estrogen receptor. FEBS Lett. 392, 49-53. https://doi.org/10.1016/0014-5793(96)00782-X
  4. Song, R. X., Zhang, Z. and Santen, R. J. (2005) Estrogen rapid action via protein complex formation involving ERalpha and Src. Trends Endocrinol. Metab. 16, 347-353. https://doi.org/10.1016/j.tem.2005.06.010
  5. Milbrandt, J. (1987) A nerve growth factor-induced gene encodes a possible transcriptional regulatory factor. Science 238, 797-799. https://doi.org/10.1126/science.3672127
  6. Duan, R., Xie, W., Burghardt, R. C. and Safe, S. (2001) Estrogen receptor-mediated activation of the serum response element in MCF-7 cells through MAPK-dependent phosphorylation of Elk-1. J. Biol. Chem. 276, 11590-11598. https://doi.org/10.1074/jbc.M005492200
  7. Chen, C. C., Lee, W. R. and Safe, S. (2004) Egr-1 is activated by 17beta-estradiol in MCF-7 cells by mitogen- activated protein kinase-dependent phosphorylation of ELK-1. J. Cell. Biochem. 93, 1063-1074. https://doi.org/10.1002/jcb.20257
  8. de Jager, T., Pelzer, T., Muller-Botz, S., Imam, A., Muck, J. and Neyses, L. (2001) Mechanisms of estrogen receptor action in the myocardium. Rapid gene activation via the ERK1/2 pathway and serum response elements. J. Biol. Chem. 276, 27873-27880. https://doi.org/10.1074/jbc.M010984200
  9. Pratt, M. A., Satkunaratnam, A. and Novosad, D. M. (1998) Estrogen activates raf-1 kinase and induces expression of Egr-1 in MCF-7 breast cancer cells. Mol. Cell. Biochem. 189, 119-125. https://doi.org/10.1023/A:1006827015320
  10. Sur, P., Sribnick, E. A., Wingrave, J. M., Nowak, M. W., Ray, S. K. and Banik, N. L. (2003) Estrogen attenuates oxidative stress-induced apoptosis in C6 glial cells. Brain Res. 971, 178-188. https://doi.org/10.1016/S0006-8993(03)02349-7
  11. Tremblay, G. B., Tremblay, A., Copeland, N. G., Gilbert, D. J., Jenkins, N. A., Labrie, F. and Giguere, V. (1997) Cloning, chromosomal localization, and functional analysis of the murine estrogen receptor beta. Mol. Endocrinol. 11, 353-365. https://doi.org/10.1210/me.11.3.353
  12. Ling, Y., Lakey, J. H., Roberts, C. E. and Sharrocks, A. D. (1997) Molecular characterization of the B-box protein- protein interaction motif of the ETS-domain transcription factor Elk-1. EMBO J. 16, 2431-2440. https://doi.org/10.1093/emboj/16.9.2431
  13. Schratt, G., Weinhold, B., Lundberg, A. S., Schuck, S., Berger, J., Schwarz, H., Weinberg, R. A., Ruther, U. and Nordheim, A. (2001) Serum response factor is required for immediate-early gene activation yet is dispensable for proliferation of embryonic stem cells. Mol. Cell. Biol. 21, 2933-2943. https://doi.org/10.1128/MCB.21.8.2933-2943.2001
  14. Watson, D. K., Robinson, L., Hodge, D. R., Kola, I., Papas, T. S. and Seth, A. (1997) FLI1 and EWS-FLI1 function as ternary complex factors and ELK1 and SAP1a function as ternary and quaternary complex factors on the Egr1 promoter serum response elements. Oncogene 14, 213-221. https://doi.org/10.1038/sj.onc.1200839
  15. Razandi, M., Pedram, A., Greene, G. L. and Levin, E. R. (1999) Cell membrane and nuclear estrogen receptors (ERs) originate from a single transcript: studies of ERalpha and ERbeta expressed in Chinese hamster ovary cells. Mol. Endocrinol. 13, 307-319. https://doi.org/10.1210/me.13.2.307
  16. Migliaccio, A., Castoria, G., Di Domenico, M., de Falco, A., Bilancio, A., Lombardi, M., Barone, M. V., Ametrano, D., Zannini, M. S., Abbondanza, C. and Auricchio, F. (2000) Steroid-induced androgen receptor-oestradiol receptor beta-Src complex triggers prostate cancer cell proliferation. EMBO J. 19, 5406-5417. https://doi.org/10.1093/emboj/19.20.5406
  17. Sukhatme, V. P., Cao, X. M., Chang, L. C., Tsai-Morris, C. H., Stamenkovich, D., Ferreira, P. C., Cohen, D. R., Edwards, S. A., Shows, T. B., Curran, T., Le Beau, M. M. and Adamson, E. D. (1988) A zinc finger-encoding gene coregulated with c-fos during growth and differentiation, and after cellular depolarization. Cell 53, 37-43. https://doi.org/10.1016/0092-8674(88)90485-0
  18. Edwards, S. A., Darland, T., Sosnowski, R., Samuels, M. and Adamson, E. D. (1991) The transcription factor, Egr-1, is rapidly modulated in response to retinoic acid in P19 embryonal carcinoma cells. Dev. Biol. 148, 165-173. https://doi.org/10.1016/0012-1606(91)90327-Y
  19. Liu, C., Rangnekar, V. M., Adamson, E. and Mercola, D. (1998) Suppression of growth and transformation and induction of apoptosis by EGR-1. Cancer Gene Ther. 5, 3-28.
  20. Aicher, W. K., Sakamoto, K. M., Hack, A. and Eibel, H. (1999) Analysis of functional elements in the human Egr-1 gene promoter. Rheumatol. Int. 18, 207-214. https://doi.org/10.1007/s002960050086
  21. Schwachtgen, J. L., Houston, P., Campbell, C., Sukhatme, V. and Braddock, M. (1998) Fluid shear stress activation of egr-1 transcription in cultured human endothelial and epithelial cells is mediated via the extracellular signal-related kinase 1/2 mitogen-activated protein kinase pathway. J. Clin. Invest. 101, 2540-2549. https://doi.org/10.1172/JCI1404
  22. Treisman, R. (1994) Ternary complex factors: growth factor regulated transcriptional activators. Curr. Opin. Genet. Dev. 4, 96-101. https://doi.org/10.1016/0959-437X(94)90097-3
  23. Sharrocks, A. D. (2002) Complexities in ETS-domain transcription factor function and regulation: lessons from the TCF (ternary complex factor) subfamily. The Colworth Medal Lecture. Biochem. Soc. Trans. 30, 1-9. https://doi.org/10.1042/bst0300001
  24. Guha, M., O'Connell, M. A., Pawlinski, R., Hollis, A., McGovern, P., Yan, S. F., Stern, D. and Mackman, N. (2001) Lipopolysaccharide activation of the MEK-ERK1/2 pathway in human monocytic cells mediates tissue factor and tumor necrosis factor alpha expression by inducing Elk-1 phosphorylation and Egr-1 expression. Blood 98, 1429-1439. https://doi.org/10.1182/blood.V98.5.1429
  25. Kim, C. G., Choi, B. H., Son, S. W., Yi, S. J., Shin, S. Y. and Lee, Y. H. (2007) Tamoxifen-induced activation of p21Waf1/Cip1 gene transcription is mediated by Early Growth Response-1 protein through the JNK and p38 MAP kinase/Elk-1 cascades in MDA-MB-361 breast carcinoma cells. Cell Signal 19, 1290-1300. https://doi.org/10.1016/j.cellsig.2007.01.008
  26. Shin, S. Y., Kim, C. G., Kim, S. H., Kim, Y. S., Lim, Y. and Lee, Y. H. (2010) Chlorpromazine activates p21Waf1/ Cip1 gene transcription via early growth response-1 (Egr-1) in C6 glioma cells. Exp. Mol. Med. 42, 395-405. https://doi.org/10.3858/emm.2010.42.5.041

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