한국응용곤충학회지 (Korean journal of applied entomology)

  • 제50권2호
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  • Pages.131-139
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  • 2011
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  • 1225-0171(pISSN)
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  • 2287-545X(eISSN)
한국응용곤충학회 (Korean Society of Applied Entomology)
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스트레스 음파 처리에 따른 아메리카잎굴파리(Liriomyza trifolii)의 생리 변화와 프로테오믹 분석

Effects of Sound Stress on Physiological Processes of the American Leafminer, Liriomyza trifolii, and Proteomic Analysis

  • 박정아 (안동대학교 자연과학대학 생명자원과학과) ;
  • ;
  • 김용균 (안동대학교 자연과학대학 생명자원과학과)
  • Park, Jung-A (School of Bioresource Science, College of Natural Science, Andong National University) ;
  • Surakasi, Venkara Prasad (School of Bioresource Science, College of Natural Science, Andong National University) ;
  • Kim, Yong-Gun (School of Bioresource Science, College of Natural Science, Andong National University)
  • 투고 : 2011.04.13
  • 심사 : 2011.06.19
  • 발행 : 2011.06.30
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초록

본 연구는 음파 처리에 따른 아메리카잎굴파리(Liriomyza trifolii)의 생리 변화를 발육 시기별로 분석하였다. 유충의 섭식량은 섭식을 통해 형성된 잎 내부 갱도의 길이로 분석했다. 이때 5,000 Hz (95 dB)의 음파 처리는 섭식 행동을 크게 둔화시켰다. 이러한 스트레스 음파는 또한 용발육에 영향을 주어 315 - 5,000 Hz 음파 범위에서 발육 지연 효과를 나타냈고, 1,000 - 5,000 Hz 음파 범위에서 우화를 억제하였다. 스트레스 음파는 암컷의 산란 행동을 억제시켰으나 성충의 주광성 행동에는 영향을 주지 않았다. 스트레스 음파(5,000 Hz, 95 dB)를 받은 용은 이차원 전기영동 분석 결과 단백질 발현 양상에서 무처리구와 뚜렷한 차이를 보였다. 특정 단백질에 대한 MALDI-TOF 분석 결과는 trafficking protein particle complex I, triosephosphate isomerase, hypothetical protein TcasGA2_TC013388, polycystin-2, paraneoplastic neuronal antigen MA1 및 tropomyosin I (isoform M)의 단백질들이 무처리구에서 발견되고, 음파 처리구에서는 소멸되었다. 반면에 DOCK9, cytoskeletal keratin II 및 $F_0F_1$-ATP synthase beta subunit은 스트레스 음파 처리에 따라 새롭게 나타나는 단백질로 판명되었다. 이러한 스트레스 음파는 아메리카잎굴파리의 살충제에 대한 감수성을 크게 증가시켰다. 이상의 결과는 아메리카잎굴파리의 생리 과정을 교란하는 스트레스 음파가 작용한다는 것을 제시하고 있다. 또한 본 연구는 이러한 스트레스 음파를 이용하여 아메리카잎굴파리에 대한 새로운 물리적 해충 방제 기술 개발 가능성을 제시하고 있다.

This study investigated the adverse effects of sound treatment on physiological processes of the American leafminer, Liriomyza trifolii, during several developmental stages. Larval feeding activity was analyzed by measuring feeding tunnel length. It was significantly suppressed by sound treatment (5,000 Hz, 95 dB). Sound treatment delayed the pupal period at 315 - 5,000 Hz and prevented adult emergence at 1,000 - 5,000 Hz. Female oviposition was also inhibited by the stress sound treatments. However, phototactic adult movement was not affected by sound treatment. Pupae treated with 5,000 Hz showed marked changes in protein patterns analyzed by two dimensional electrophoresis. MALDI-TOF analysis of specific protein spots indicated that trafficking protein particle complex I, triosephosphate isomerase, hypothetical protein TcasGA2_TC013388, polycystin-2, paraneoplastic neuronal antigen MA1, and tropomyosin I (isoform M) were predicted in the control insects and disappeared in the insects treated with sound. By contrast, DOCK9, cytoskeletal keratin II, and F0F1-ATP synthase beta subunit were predicted only in the sound-treated insects. Furthermore, stress sound significantly increased the susceptibility of L. trifolii to insecticides. These results suggest that physiological processes of L. trifolii are altered by sound stress, which may be exploited to develop a novel physical control tactic against L. trifolii.

키워드

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피인용 문헌

  1. Biochemical Analysis of Physiological Stress Induced by High Frequency Sound Treatment in the Beet Armyworm, Spodoptera exigua vol.51, pp.3, 2012, https://doi.org/10.5656/KSAE.2012.06.0.009
  2. Analysis of Physiological Alterations in Development and Mating Behavior by Ultrasound Treatment in the Beet Armyworm, Spodoptera exigua vol.51, pp.3, 2012, https://doi.org/10.5656/KSAE.2012.05.0.029
  3. Effect of Stress Sound on the Development of the Black Soldier Fly, Hermetia illucens vol.52, pp.3, 2013, https://doi.org/10.5656/KSAE.2013.06.0.025