Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Sound Stress on Physiological Processes of the American Leafminer, Liriomyza trifolii, and Proteomic Analysis

스트레스 음파 처리에 따른 아메리카잎굴파리(Liriomyza trifolii)의 생리 변화와 프로테오믹 분석

  • Park, Jung-A (School of Bioresource Science, College of Natural Science, Andong National University) ;
  • Surakasi, Venkara Prasad (School of Bioresource Science, College of Natural Science, Andong National University) ;
  • Kim, Yong-Gun (School of Bioresource Science, College of Natural Science, Andong National University)
  • 박정아 (안동대학교 자연과학대학 생명자원과학과) ;
  • ;
  • 김용균 (안동대학교 자연과학대학 생명자원과학과)
  • Received : 2011.04.13
  • Accepted : 2011.06.19
  • Published : 2011.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study investigated the adverse effects of sound treatment on physiological processes of the American leafminer, Liriomyza trifolii, during several developmental stages. Larval feeding activity was analyzed by measuring feeding tunnel length. It was significantly suppressed by sound treatment (5,000 Hz, 95 dB). Sound treatment delayed the pupal period at 315 - 5,000 Hz and prevented adult emergence at 1,000 - 5,000 Hz. Female oviposition was also inhibited by the stress sound treatments. However, phototactic adult movement was not affected by sound treatment. Pupae treated with 5,000 Hz showed marked changes in protein patterns analyzed by two dimensional electrophoresis. MALDI-TOF analysis of specific protein spots indicated that trafficking protein particle complex I, triosephosphate isomerase, hypothetical protein TcasGA2_TC013388, polycystin-2, paraneoplastic neuronal antigen MA1, and tropomyosin I (isoform M) were predicted in the control insects and disappeared in the insects treated with sound. By contrast, DOCK9, cytoskeletal keratin II, and F0F1-ATP synthase beta subunit were predicted only in the sound-treated insects. Furthermore, stress sound significantly increased the susceptibility of L. trifolii to insecticides. These results suggest that physiological processes of L. trifolii are altered by sound stress, which may be exploited to develop a novel physical control tactic against L. trifolii.

본 연구는 음파 처리에 따른 아메리카잎굴파리(Liriomyza trifolii)의 생리 변화를 발육 시기별로 분석하였다. 유충의 섭식량은 섭식을 통해 형성된 잎 내부 갱도의 길이로 분석했다. 이때 5,000 Hz (95 dB)의 음파 처리는 섭식 행동을 크게 둔화시켰다. 이러한 스트레스 음파는 또한 용발육에 영향을 주어 315 - 5,000 Hz 음파 범위에서 발육 지연 효과를 나타냈고, 1,000 - 5,000 Hz 음파 범위에서 우화를 억제하였다. 스트레스 음파는 암컷의 산란 행동을 억제시켰으나 성충의 주광성 행동에는 영향을 주지 않았다. 스트레스 음파(5,000 Hz, 95 dB)를 받은 용은 이차원 전기영동 분석 결과 단백질 발현 양상에서 무처리구와 뚜렷한 차이를 보였다. 특정 단백질에 대한 MALDI-TOF 분석 결과는 trafficking protein particle complex I, triosephosphate isomerase, hypothetical protein TcasGA2_TC013388, polycystin-2, paraneoplastic neuronal antigen MA1 및 tropomyosin I (isoform M)의 단백질들이 무처리구에서 발견되고, 음파 처리구에서는 소멸되었다. 반면에 DOCK9, cytoskeletal keratin II 및 $F_0F_1$-ATP synthase beta subunit은 스트레스 음파 처리에 따라 새롭게 나타나는 단백질로 판명되었다. 이러한 스트레스 음파는 아메리카잎굴파리의 살충제에 대한 감수성을 크게 증가시켰다. 이상의 결과는 아메리카잎굴파리의 생리 과정을 교란하는 스트레스 음파가 작용한다는 것을 제시하고 있다. 또한 본 연구는 이러한 스트레스 음파를 이용하여 아메리카잎굴파리에 대한 새로운 물리적 해충 방제 기술 개발 가능성을 제시하고 있다.

Keywords

References

  1. Bradford, M.M. 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 71: 248-254.
  2. Cai, Y., Y. Maeda, A. Cedzich, V.E. Torres, G. Wu, T. Hayashi, T. Mochizuki, J.H. Park, R. Witzgall and S. Somlo. 1999. Identification and characterization of polycystin-2, the PKD2 gene product. J. Biol. Chem. 274: 28557-28565. https://doi.org/10.1074/jbc.274.40.28557
  3. Chandler, L.D. and F.E. Gilstrap. 1986. Within-plant larva distribution of Liriomyza trifolii Burgess (Diptera: Agromyzidae) on Bell peppers. Environ. Entomol. 15: 96-99. https://doi.org/10.1093/ee/15.1.96
  4. Chen, H.C. and S.R. D'Mello. 2010. Induction of neuronal cell death by paraneoplastic Ma1 antigen. J. Neurosci. Res. 88: 3508-3519. https://doi.org/10.1002/jnr.22506
  5. Furguson, J.S. 2004. Development and stability of insecticide resistance in the leafminer Liriomyza trifolii (Diptera: Agromyzidae) to cyromazine, abamectin, and spinosad. J. Econ. Entomol. 97: 112-119. https://doi.org/10.1603/0022-0493-97.1.112
  6. Han, M.J., S.H. Lee, J.Y. Choi, S.B. Ahn and M.H. Lee. 1996. Newly introduced insect pest, american serpentine leafminer, Liriomyza trifolii (Burgess) (Diptera: Agromyzidae) in Korea. Kor. J. Appl. Entomol. 35: 309-314.
  7. Hoffmeister, H., A.R. Gallagher, A. Rascle and R. Witzgall. 2010. The human polycystin-2 protein represents an integral membrane protein with six membrane-spanning domains and intracellular N- and C-termini. Biochem. J. 433: 285-294.
  8. Kamimura, M. and M. Kiuchi. 2002. Applying fenoxycarb at the penultimate instar triggers an additional ecdysteroid surge and induces perfect extra larval molting in the silkworm. Gen. Comp. Endocrinol. 128: 231-237. https://doi.org/10.1016/S0016-6480(02)00507-5
  9. Kim, J.H., Y.W. Byoun, G.S. Lee and H.Y. Kim. 2007. Evaluation of biological control of Liriomyza trifolii (Burgess) (Diptera: Agromyzidae) using Diglyphus isaea (Walker) (Hymenoptera: Eulophidae) in three seasonal culture types of tomato greenhouse. Kor. J. Appl. Entomol. 46: 71-78. https://doi.org/10.5656/KSAE.2007.46.1.071
  10. Kim, Y., D. Kim and J. Lee. 2000. Disturbance of adult eclosion by fenoxycarb, a juvenile hormone mimic, in the beet armyworm, Spodoptera exigua. J. Asia Pac. Entomol. 3: 103-111. https://doi.org/10.1016/S1226-8615(08)60063-1
  11. Ku, N.O., D.M. Toivola, P. Strand and M.B. Omary. 2010. Cytoskeletal keratin glycosylation protects epithelial tissue from injury. Nat. Cell Biol. 12: 876-885. https://doi.org/10.1038/ncb2091
  12. Lim, J.R., H.C. Moon, S.U. Choi, J.H. Kim, K.K. Lee, B.R. Ko, J.S. Choi, Y.K. Jeon and C.Y. Hwang. 2007. Economic injury levels of Liriomyza trifolii Burgess (Diptera: Agromyzidae) infesting eggplant in greenhouse. Kor. J. Appl. Entomol. 46: 409-414. https://doi.org/10.5656/KSAE.2007.46.3.409
  13. Liu, X., Y. Wang, H. Zhu, Q. Zhang, X. Xing, B. Wu, L. Song and L. Fan. 2010. Interaction of sedlin with PAM14. J. Cell Biochem. 109: 1129-1133.
  14. Montpetit, B. and E. Conibear. 2009. Identification of the novel TRAPP associated protein Tca17. Traffic. 10: 713-723. https://doi.org/10.1111/j.1600-0854.2009.00895.x
  15. Moon, H.C., J.S. Choi and C.Y. Hwang. 2002. Ovipositional characteristics of Hemiptarsenus zilahisebessi (Hymenoptera: Eulophidae), ectoparasitoid of Liriomyza trifolii (Diptera: Agromyzidae). Kor. J. Appl. Entomol. 41: 21-25.
  16. Park, J.D., Y.S. Ku, D.S. Choi and S.S. Kim. 2001. Damaged aspects, seasonal fluctuations, and attractivity of various colors on Liriomyza trifolii Burgess (Diptera: Agromyzidae) in Gerbera. Kor. J. Appl. Entomol. 40: 97-103.
  17. Park, J.D., H.B. Lee, S.G. Kim, D.I. Kim, I.J. Park, S.C. Kim and K.C. Kim. 2000. Population dynamics and injuries by Liriomyza trifolii (Burgess) in chrysanthemum field. Kor. J. Appl. Entomol. 39: 141-147.
  18. Parrella, M.P. 1987. Biology of Liriomyza. Annu. Rev. Entomol. 32: 201-224. https://doi.org/10.1146/annurev.en.32.010187.001221
  19. Parrella, M.P. and C.B. Keil. 1984. Insect pest management: the lesson of Liriomyza. Annu. Rev. Entomol. 32: 201-224.
  20. SAS Institute. 1989. SAS/STAT User's Guide, Release 6.03, Ed. Cary, NC, USA.
  21. Shin, H.J., M.J. Seo and Y.N. Youn. 2006. Ultrastructure of antennal sensilla on american leafminer, Liriomyza trifolii (Diptera: Agromyzidae). Kor. J. Appl. Entomol. 45: 119-129.
  22. Singh, V.B., K.C. Corley, T.H. Phan and M.C. Boadle-Biber. 1990. Increases in the activity of tryptophan hydroxylase from rat cortex and midbrain in response to acute or repeated sound stress are blocked by adrenalectomy and restored by dexamethasone treatment. Brain Res. 516: 66-76. https://doi.org/10.1016/0006-8993(90)90898-L
  23. Trumble, J.T. 1990. Vegetable insect control with minimal use of insecticides. Hortscience 25: 159-163.
  24. Trumble, J.T., I.P. Ting and L. Bates. 1985. Analysis of physiological growth and yield response of celery to Liriomyza trifolii. Entomol. Exp. Appl. 38: 15-21. https://doi.org/10.1111/j.1570-7458.1985.tb03492.x
  25. Wei, J., L. Zou, R. Kuang and L. He. 2000. Influence of leaf tissue structure on host feeding selection by pea leafminer Liriomyza huidobrensis (Diptera: Agromyzidae). Zool. Studies 39: 295-300.
  26. Yang, J., Z. Zhang, S.M. Roe, C.J. Marshall and D. Barford. 2009. Activation of Rho GTPases by DOCK exchange factors is mediated by a nucleotide sensor. Science 325: 1398-1402. https://doi.org/10.1126/science.1174468

Cited by

  1. Biochemical Analysis of Physiological Stress Induced by High Frequency Sound Treatment in the Beet Armyworm, Spodoptera exigua vol.51, pp.3, 2012, https://doi.org/10.5656/KSAE.2012.06.0.009
  2. Analysis of Physiological Alterations in Development and Mating Behavior by Ultrasound Treatment in the Beet Armyworm, Spodoptera exigua vol.51, pp.3, 2012, https://doi.org/10.5656/KSAE.2012.05.0.029
  3. Effect of Stress Sound on the Development of the Black Soldier Fly, Hermetia illucens vol.52, pp.3, 2013, https://doi.org/10.5656/KSAE.2013.06.0.025