Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Antioxidant and Anti-Inflammatory Activities of Extracts of Traditional Medicinal Plants Mixtures 1 and 2

약용식물 복합 추출물 TMP1과 TMP2의 항산화능과 염증 억제 효과

  • Cho, Eun-Kyung (Department of Food and Nutrition, College of Medical Life Sciences, Silla University) ;
  • Choi, Young-Ju (Department of Food and Nutrition, College of Medical Life Sciences, Silla University)
  • 조은경 (신라대학교 의생명과학대학 식품영양학과) ;
  • 최영주 (신라대학교 의생명과학대학 식품영양학과)
  • Received : 2011.03.15
  • Accepted : 2011.04.01
  • Published : 2011.04.30
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Abstract

This study was conducted to investigate traditional medicinal plants (TMP) 1 and 2, two different multi-herbal mixtures consisting of 24 herbs. Regarding the contents of flavonoid compounds, the ethanol extract (EE) of TMP2 yielded the highest content of flavonoid compounds (40.981 mg/g), followed by EE of TMP1 (28.23 mg/g), hot water extract of TMP2 (WE, 10.046 mg/g), and WE of TMP1 (6.59 mg/g). Antioxidant activities of EE and WE of TMP1 and TMP2 were measured based on DPPH radical scavenging activity. At 1 mg/ml, the highest DPPH radical scavenging activity was shown in EE of TMP2 (96%), followed by EE of TMP1 (94%). Nitric oxide (NO) production by RAW264.7 macrophage cells stimulated with lipopolysaccharide (LPS) was reduced to 82, 77, 86, and 47% by addition of $500\;{\mu}g/ml$ of EE and WE of TMP1 and TMP2, respectively. These results were not due to the cytotoxicity of the extracts. NO synthesis was increased by 2.3, 3.6, 3.0, and 1.8 fold by addition of $500\;{\mu}g/ml$ of EE and WE of TMP1 and TMP2, respectively. These results show that medicinal plants play a significant role in antioxidative activity and activation of the immune system in the pathogenesis of inflammatory diseases, and different mixtures of the medicinal plants showed different effects.

본 연구에서는 약용식물 TMP1과 TMP2의 기능성에 관한 분석을 위하여 열수, 에탄올 추출물로 여러 가지 생리활성과 면역활성에 대하여 분석하였다. 우선, TMP1과 TMP2의 항산화능을 플라보노이드 함량 측정으로 예측하고자 TMP1과 TMP2의 열수, 에탄올 추출물의 총 플라보이드 함량을 조사하였다. 그 결과, TMP2 에탄올 추출물이 40.981 mg/g으로 가장 높았으며, 이는 높은 항산화능과 연관성이 있는 것으로 나타났는데, DPPH법을 통해 측정한 TMP2의 항산화력은 1 mg/ml 농도에서 에탄올 추출물이 96%의 radical 소거능을 나타내었다. 0.1 mg/ml의 비타민 C와 비교 했을 때 유사한 항산화력이 측정되었는데, 이것은 높은 항산화능을 나타내고 있는 것으로 TMP2의 높은 이용가치를 의미한다. TMP2와 면역활성과의 연관성은 LPS에 의해 유도되는 NO 합성 저해률과 TMP2에 의한 대식세포의 NO 합성 증진율 분석으로 조사되었다. 그 결과 LPS에 의해 유도된 NO 합성 저해율은 0.5 mg/ml 농도에서 TMP2 열수, 에탄올 추출물이 각각 47%, 86%로 나타나 약용식물 TMP2의 높은 면역효과를 증명하고 있다. 또한 TMP2 에탄올 추출물은 3배의 높은 NO 합성률을 보였다. 지금까지의 연구에 의하면 NO 합성율 증대로 인한 약용식물 추출물의 항염증 효능에 대한 보고가 미비하므로 본 연구는 학술적의미가 크다고 판단된다. 이상의 결과는 약용식물 TMP2의 우수한 생리활성을 증명하고 있고, 항산화력, 면역활성 효과가 높은 것으로 나타나 기능성 음료의 소재로서 그 활용도가 높을 것으로 판단된다.

Keywords

References

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