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해조류 추출물의 항산화 및 nitrite 소거활성 평가

Evaluation of Antioxidant and Nitrite Scavenging Activity of Seaweed Extracts

  • 안선미 (안동대학교 식품영양학과) ;
  • 홍용기 (부경대학교 생물공학과) ;
  • 권기석 (안동대학교 생명자원과학부) ;
  • 손호용 (안동대학교 식품영양학과)
  • Ahn, Seon-Mi (Department of Food and Nutrition, Andong National University) ;
  • Hong, Yong-Ki (Department of Biotechnology, Pukyong National University) ;
  • Kwon, Gi-Seok (The School of Bioresource Science, Andong National University) ;
  • Sohn, Ho-Yong (Department of Food and Nutrition, Andong National University)
  • 투고 : 2011.01.25
  • 심사 : 2011.04.01
  • 발행 : 2011.04.30

초록

유용생리활성의 해조류를 이용한 식품소재 개발 연구의 일환으로, 35종 해조류(갈조류 17종, 홍조류 11종 및 녹조류 7종)의 메탄올 추출물을 대상으로 in-vitro 항산화 및 nitrite 소거활성을 평가하였다. 해조류 추출물($500\;{\mu}g/ml$) 중 감태, 곰피, 대황, 넓패, 패, 모자반, 알송이모자반, 야마자모자반 및 잘피의 9종에서 60% 이상의 DSA 활성을 나타내었으며, ASA활성은 DSA활성과 높은 상관관계를 나타내었다(상관계수 0.855). 환원력 평가 결과에서는 감태, 곰피 및 대황의 3종만이 0.88 ($Abs_{700}$) 이상의 강력한 활성을 나타내었다. 한편 식품에서의 발암인자인 nitrosoamine생성억제와 관련된 NSA평가의 경우, 전체 35종의 해조류 중 19종에서 60% 이상의 NSA를 나타내었으며, 특히 기존에 알려지지 않은 모자반, 야마다모자반, 알송이모자반, 패, 넓패에서 우수한 활성을 확인하였다. 1차 선별된 9종 해조류의 DSA, ASA, NSA의 $IC_{50}$를 조사한 결과, 기존의 항산화능이 알려진 곰피, 대황, 감태 이외에도 야마다모자반 및 넓패에서 강력한 항산화 활성과 nitrite 소거활성을 확인하였다. 본 연구결과는 야마다 모자반 및 넓패를 이용하여 항혈전, 항산화, NSA 활성을 동시에 나타내는 기능성 식품소재로의 개발이 가능함을 제시하고 있다.

In the course of study for the development of functional food ingredients from seaweeds having useful biological activities, the in-vitro antioxidant and nitrite scavenging activities of the methanol extracts prepared from 35 different seaweeds (17 phaeophyta, 11 rhodophyta and 7 chlorophyta) were determined. At $500\;{\mu}g/ml$ concentration of the methanol extracts, Ecklonia cava, Ecklonia stolonifera, Eisenia bicyclis (Kjellman) Setchell, Ishige foliacea, I. okamurai, Sargassum confusum, S. fulvellum, S. yamade and Zostera marina showed 60% more DPPH and ABTS scavenging activity. The exceptions were found in I. okamurai and Z. marina, which showed 45% and 53% ABTS scavenging activity, respectively. The correlation coefficient between DPPH and ABTS scavenging activities was 0.855, suggesting that the 9 different seaweeds extracts could scavenge anion and cation radicals concurrently. In reducing power, only E.cava, E.stolonifera and E.bicyclis showed above 0.88 ($Abs_{700}$). In a while, in nitrite scavenging activity at $100\;{\mu}g/ml$ concentration of the methanol extracts, 19 different seaweeds extracts including I. foliacea, I. okamurai, S. confusum, S. fulvellum, and S. yamade showed 60% more activity. Calculation of $IC_{50}s$ of DPPH, ABTS and nitrite scavenging activities of 9 different seaweeds extracts further showed that I. foliacea and S. yamade, besides E.cava, E.stolonifera and E.bicyclis, have strong antioxidant and nitrite scavenging activity. These results suggest that the selected 9 different seaweeds could be developed as functional food ingredients and I. foliacea and S. yamade have potential as novel natural sources of antioxidant and nitrite scavenger.

키워드

참고문헌

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