Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Immunomodulating Effect of Edible Mushrooms in Mice

식용버섯의 면역조절에 미치는 영향

  • Park, Hyun-Ji (Chemon Inc.) ;
  • Heo, Yong (Department of Occupational Health, Catholic University of Daegu) ;
  • Kim, Jong-Bong (Department of Biomedical Science, Catholic University of Daegu)
  • 박현지 (켐온(주)) ;
  • 허용 (대구가톨릭대학교 산업보건학과) ;
  • 김종봉 (대구가톨릭대학교 의생명과학과)
  • Received : 2010.11.26
  • Accepted : 2011.04.01
  • Published : 2011.04.30
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Abstract

This study was carried out to evaluate the immunomodulatory capacity of edible mushrooms, including Lepista nuda, Corprinis comatus, Letinus edodes, and Pleurotus eryngii, in mice. BALB/c mice were administered 1, 50, and 500 mg/kg body weight of various mushrooms five times a week over 4 weeks through gastric intubation. The control mice were administered distilled water. No significant changes in body weight were observed. IL-4 and IFN${\gamma}$ production was evaluated with splenic T lymphocytes stimulated in vitro with phytohemagglutinins for 48 hr. The mice group administered L. edodes showed significantly higher ratio of IFN${\gamma}$ versus IL-4 than the other groups. In addition, the ratio of plasma IgG2a versus IgG1 was also significantly elevated in mice treated with L. edodes. However, no significant change was observed in ratio of IgG2a versus IgG1 in splenic B lymphocytes stimulated in vitro with lipopolysaccharides for 7 days. These results indicate that L. edodes can enhance type-1 helper T cell-mediated cellular immunity.

본 연구는 식용버섯들의 면역조절기능제로서의 가치 여부를 평가하기 위하여 수행되었다. 본 연구를 위해 민자주방망이버섯, 먹물버섯, 표고버섯, 새송이버섯이 사용되었다. 버섯을 투여한 생쥐의 혈장 내 IgG1, IgG2a 수준을 측정하였고, 비장 단일 세포군을 이용하여 T 림프구 및 B 림프구 in vitro 활성화 결과 생성된 IFN-${\gamma}$와 IL-4, IgG1과 IgG2a 수준을 각각 분석하였다. 실험 결과 표고버섯 1 mg/체중 kg을 투여한 군에서 IFN${\gamma}$/IL-4의 비가 다른 군에 비해 유의하게 높았다. 또한 혈장 내 IgG2a/IgG1의 비가 표고버섯의 경우에 다른 군보다 높았다. 아울러 TNF${\alpha}$의 생성 역시 표고버섯 1 mg/체중 kg을 투여한 군에서 다른 군보다 높았다. 이는 표고버섯이 항암작용, 항바이러스 작용과 같은 type-1 반응을 촉진할 가능성이 있음을 예측케 해주는 결과라 생각된다.

Keywords

References

  1. Adachi, Y., Y. Suzuki, T. Jinushi, T. Yadomae, and N. Ohno. 2002. Th1-oriented immunomodulating activity of gel-forming fungal (1,3)-beta-glucans. Int. J. Med. Mushrooms 4, 95-109.
  2. Borchers, A. T., C. L. Keen, and M. E. Gershwin. 2004. Mushrooms, tumors and immunity:an update. Exp. Biol. Med. 229, 393-406.
  3. Fan, L., H. Pan, A. T. Soccol, A. Pandey, and C. R. Soccol. 2006. Advances in mushroom research in the last decade. Food Technol. Biotechnol. 44, 303-311.
  4. Furue, H. and I. Kitoh. 1981. Phase 111-study on Lentinan. Jp. J. Cancer Chemother. 8, 944-960.
  5. Heo, Y., P. Parsons, and D. A. Lawrence. 1996. Lead differentically modifies cytokine production in vitro and in vivo. Taxocol. Appl. Pharmacol. 138, 149-157. https://doi.org/10.1006/taap.1996.0108
  6. Hsieh, K. Y., C. I. Hsu, J. Y. Lin, C. C. Tsai, and R. H. Lin. 2003. Oral administration of an edible-mushroom-derived protein inhibits the development of food-allergic reactions in mice. Clin. Exp. Allergy 33, 1593-1602.
  7. Hsu, H. C., C. I. Hsu, R. H. Lin, C. L. Kao, and J. Y. Lin. 1997. Fip-vvo, a new fungal immunomodulatory protein isolated from Volvariella volvacea. Biochem. J. 323, 557-565.
  8. Kodama, N., K. Komuta, and H. Nanba. 2003. Effect of Maitake (Grifola frondosa) D-fraction in the activation of NK cells in cancer patients. J. Med. Food 6, 371-377. https://doi.org/10.1089/109662003772519949
  9. Kodama, N., K. Komuta, N. Sakai, and H. Nanba. 2002. Effects of D-fraction, a polysaccharide from Grifola frondosa on tumor growth onvolves activation of NK cells. Biol. Pharm. Bull. 25, 1647-1650. https://doi.org/10.1248/bpb.25.1647
  10. Leung, M. Y., K. P. Fung, and Y. M. Choy. 1997. The isolation and characterization of an immunomodulatory and anti-tumor polysaccharide preparation from Flammulina velutipes. Immunopharmacol. 35, 255-263. https://doi.org/10.1016/S0162-3109(96)00157-9
  11. Liu, J. J., T. S. Huang, M. L. Hsu, C. C. Chen, W. S. Lin, F. J. Lu, and W. H. Chang. 2004. Antitumor effects of the partially purified polysaccharides from Antrodia camphorate and the mechanism of its action. Toxicol. Appl. Pharmacol. 201, 186-193. https://doi.org/10.1016/j.taap.2004.05.016
  12. Lull, C., H. J. Wichers, and H. F. J. Savelkoul. 2005. Antiinflammatory and immunomodulation properties of fungal metabolites. Mediat. Inflamm. 2, 63-80.
  13. Mizuno, M., S. Kawakami, and N. Fujitake. 2003. Macrophages stinolated by polysaccharide purified from Agaricus brasiliensis S. Wasser et al. (Agaricomycetideae) enhance mRNA expression of Th1 cytokine Including IL-12 and 18. Int. J. Med. Mushrooms 5, 383-389. https://doi.org/10.1615/InterJMedicMush.v5.i4.50
  14. Moradali, M. F., H. Mostafavi, S. Ghods, and G. A. Hedgaroude. 2007. Immunomodulating and anticancer agent in the realm of macromycetes fungi (macrofungi). Int. Immunopharmacol. 7, 701-724. https://doi.org/10.1016/j.intimp.2007.01.008
  15. Oh, S. I. and M. S. Lee. 2005. Effects of antioxidative stress, antimutagenicity and cytotoxicity of cancer cells in fractional extracts from Ganoderma lucidum Karst. Korean J. Food Cookery Sci. 21, 759-768.
  16. Ohmori, T., A. Tamura, A. Wakaiki, G. Kawanishi, S. Tsuru, T. Yadomae, and K. Nomoto. 1988. Dissociation of a gluca fraction (CO-1) from protein-bound polysaccharide of Cordyceps ophioglossoides and analysis of its antitumor effect. Chem. Pharm. Bull. 36, 4512-4518. https://doi.org/10.1248/cpb.36.4512
  17. Park, E. M., S. J. Kim, E. J. Ye, M. J. Bae, and K. C. Jo. 2005. Effect of mycelia extracts of mushroom-cultured ginseng by-product on proliferation in cancer cell lines. J. Korean Soc. Food Sci. Nutr. 34, 323-329. https://doi.org/10.3746/jkfn.2005.34.3.323
  18. Supper, C. M. and W. E. Paul. 1987. Interferon and B cell stimulatory factor-1 reciprocally regulated Ig isotype production. Science 236, 944-947. https://doi.org/10.1126/science.3107127
  19. Taguchi, T., H. Furue, T. Kimura, T. Kondo, T. Hattori, T. Itoh, and N. Ogawa. 1985. End point results of phase III study of lentinan. Jp. J. Cancer Chemother. 12, 366-380.
  20. Taguchi, T., H. Furue, T. Kimura, T. Kondo, T. Hattori, I. Itoh, and N. Ogawa. 1985. End point result of a randomized controlled study of the treatment of gastrointestinal cencer with a combination of lentinan and chemotherapeutic agents. Excerpta Medica 40, 151-165.
  21. Tsunda, A. and N. Ishida. 1970. A mushroom extracts as an interferon inducer. Ann. N.Y. Acad. Sci. 173, 719-726. https://doi.org/10.1111/j.1749-6632.1970.tb53464.x
  22. Tzianabos, A. O. 2000. Polysaccharide immunomodulators as therapeutic agents: structural aspects and biologic function. Clin. Microbiol. Rev. 13, 523-533. https://doi.org/10.1128/CMR.13.4.523-533.2000
  23. Wasser, S. P. 2002. Medical mushrooms as a source of antitumor and immunomodulating polysaccharides. Appl. Microbiol. Biotechnol. 60, 258-274. https://doi.org/10.1007/s00253-002-1076-7
  24. Yashino, S., T. Tabata, S. Hazama, N. Iizuka, K. Yamamoto, M. Hirayama, A. Tangoku, and M. Oka. 2000. Immunoregulatory effects of the antitumor polysaccharide lentinan on Th1/Th2 balances on patients with digestive cancers. Anticancer Res. 20, 4707-4711.
  25. Yoon, Y. H. 2005. Effects of oral administration of Phellinus linteus on the productions of the Th1-and Th2-type cytokines in mice. J. Life Sic. Nat. Res. 27, 63-74.

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