Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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Comparison of Cuticular Hydrocarbons of Different Developmental Stages of the Spot Clothing Wax Cicada, Lycorma delicatula (Hemiptera: Fulgoridae)

꽃매미(Lycorma delicatula)의 발육단계별 표피탄화수소 비교

  • Cho, Sun-Ran (Dept. of Plant Medicine, Coll. of Agri. Life and Environ. Sci., Chungbuk National University) ;
  • Lee, Jeong-Eun (Dept. of Plant Medicine, Coll. of Agri. Life and Environ. Sci., Chungbuk National University) ;
  • Jeong, Jin-Won (Dept. of Plant Medicine, Coll. of Agri. Life and Environ. Sci., Chungbuk National University) ;
  • Yang, Jeong-Oh (Dept. of Plant Medicine, Coll. of Agri. Life and Environ. Sci., Chungbuk National University) ;
  • Yoon, Chang-Mann (Dept. of Plant Medicine, Coll. of Agri. Life and Environ. Sci., Chungbuk National University) ;
  • Kim, Gil-Hah (Dept. of Plant Medicine, Coll. of Agri. Life and Environ. Sci., Chungbuk National University)
  • 조선란 (충북대학교 농업생명환경대학 식물의학과) ;
  • 이정은 (충북대학교 농업생명환경대학 식물의학과) ;
  • 정진원 (충북대학교 농업생명환경대학 식물의학과) ;
  • 양정오 (충북대학교 농업생명환경대학 식물의학과) ;
  • 윤창만 (충북대학교 농업생명환경대학 식물의학과) ;
  • 김길하 (충북대학교 농업생명환경대학 식물의학과)
  • Received : 2011.05.31
  • Accepted : 2011.07.20
  • Published : 2011.09.30
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Abstract

Aliphatic cuticular hydrocarbons (CHCs) of different developmental stages of the spot clothing wax cicada, Lycorma delicatula (Hemiptera: Fulgoridae) were analyzed using GC and GC-MS. The numbers of carbons in the major CHCs of each developmental stage 32, 33, 28, 38, 37 in the egg, 1st, 2nd, 3rd, and 4th instar nymphal stages, and adults, respectively. The cuticle of Lycorma delicatula contains mainly methyl-branched 9-methylheptacosane (15.11%) in the egg stage, and a high proportion of n-heptacosane in nymphal stages (15.75, 22.42, 25.04, and 23.11 % in the 1st, 2nd, 3rd and 4th instars, respectively). In contrast, male and female adults had high proportions of n-nonacosane (13.42 and 16.55%). The chemical constituents of CHCs were classified into five groups (n-alkanes, monomethylalkanes, dimethylalkanes, trimethylalkanes, olefins) and group profiles of each developmental stage were compared. Egg surface was composed mainly monomethylalkanes (45.39%), a saturated hydrocarbon. Nymph CHCs consisted primarily of n-alkanes (37.63 to 46.12%). There was a difference between adult male and female CHCs. However, both contained n-alkanes and monomethylalkanes. CHCs with trimethyl or double bonded structure were rare in all stages.

꽃매미의 발육단계별 표피탄화수소를 비교하기 위하여 GC와 GC-MS를 이용하여 분석하였다. 분석된 꽃매미의 표피탄화수소의 종류는 알이 32종, 약충은 령별로 각각 33, 28, 38, 37종이었고 2령 약충에서 가장 적게 나타났다. 반면 성충은 암 수 모두 46종으로 동일하였으며, 꽃매미의 충태 중 가장 많은 종류의 탄화수소를 함유하고 있었다. 발육단계별 물질 함량을 분석한 결과, 알은 메틸기를 갖고 있는 9-methylheptacosane을 가장 많이 함유하고 있었으며(15.11%), 령기별 약충은 n-heptacosane을 가장 많이 함유하고 있었다(15.75, 22.42, 25.04, 23.11%). 반면 성충은 암 수 모두 n-nonacosane을 가장 많이 함유하고 있었다(13.42, 16.55%). 표피탄화수소의 구성물질을 5개 그룹(n-alkanes, monomethylalkanes, dimethylalkanes, trimethylalkanes, olefins)으로 나누고 물질 함량을 꽃매미 발육단계별로 비교하였다. 꽃매미의 알 표피는 대부분이 monomethylalkanes인 포화탄화수소로 이루어져 있고(45.39%), 약충은 포화탄화수소의 기본구조인 n-alkanes이 대부분이었다(37.63-46.12%). 또한 성충은 암 수에 따라 차이가 있었지만, n-alkanes과 monomethylalkanes을 고루 함유하는 것으로 나타났다. 반면 모든 충태에서, 3개이상의 메틸기를 갖거나 이중 결합을 포함하는 구조는 극히 적었다.

Keywords

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