Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
권호 표지

Isolation and Characterization of a Doritaenopsis Hybrid GIGANTEA Gene, Which Possibly Involved in Inflorescence Initiation at Low Temperatures

  • Luo, Xiaoyan (School of Landscape Architecture, Zhejiang Agriculture & Forestry University) ;
  • Zhang, Chi (School of Agriculture and Food Science, Zhejiang Agriculture & Forestry University) ;
  • Sun, Xiaoming (School of Landscape Architecture, Zhejiang Agriculture & Forestry University) ;
  • Qin, Qiaoping (School of Agriculture and Food Science, Zhejiang Agriculture & Forestry University) ;
  • Zhou, Mingbin (The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture & Forestry University) ;
  • Paek, Kee-Yoeup (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University) ;
  • Cui, Yongyi (School of Agriculture and Food Science, Zhejiang Agriculture & Forestry University)
  • Received : 2010.12.13
  • Accepted : 2011.02.10
  • Published : 2011.04.30
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Abstract

In the Doritaenopsis hybrid, like most of the orchid species and hybrids, temperature is crucial for the vegetative-to-reproductive transition, and low temperature is required for bud differentiation. To understand the molecular mechanism of this process, an orchid GIGANTEA (GI) gene, DhGI1, was isolated and characterized by using the rapid amplification of cDNA ends (RACE) PCR technique. Sequence analysis showed that the full-length cDNA is 4,022 bp with a major open reading frame of 3,483 bp, and the amino acid sequence showed high similarity to GI proteins in Zea mays, Oryza sativa, Arabidopsis thaliana and other plants. Semi-quantitative RT-PCR revealed that DhGI1 was expressed throughout development and could be detected in roots, stems, leaves, peduncles and flower buds. The expression level of DhGI1 was higher when the plants were flowering at low temperature (22/$18^{\circ}C$ day/night) than the other growth stages. Further analysis indicated that the accumulation of DhGI1 transcripts was significantly increased at low temperature, and concomitantly, initiation of the peduncle was observed. However, DhGI1 levels were low under high temperature (30/$25^{\circ}C$) conditions, and flower initiation was inhibited. These results indicate that the expression of DhGI1 is regulated by low temperature and that DhGI1 may play an important role in inflorescence initiation in this Doritaenopsis hybrid at low temperatures.

Keywords

References

  1. Abe, M., M. Fujiwara, K. Kurotani, S. Yokoi, and K. Shimamoto. 2008. Identification of dynamin as an interactor of rice GIGANTEA by tandem affinity purification (TAP). Plant Cell Physiol. 49:420-432. https://doi.org/10.1093/pcp/pcn019
  2. Balasubramanian, S., S. Sureshkumar, J. Lempe, and D. Weigel. 2006. Potent induction of Arabidopsis thaliana flowering by elevated growth temperature. PLoS Genet. 2:e106. https://doi.org/10.1371/journal.pgen.0020106
  3. Blanchard, M.G. and E.S. Runkle. 2006. Temperature during the day, but not during the night, controls flowering of Phalaenopsis orchids. J. Exp. Bot. 57:4043-4049. https://doi.org/10.1093/jxb/erl176
  4. Cao, S., M. Ye, and S. Jiang. 2005. Involvement of GIGANTEA gene in the regulation of the cold stress response in Arabidopsis. Plant Cell Rep. 24:683-690. https://doi.org/10.1007/s00299-005-0061-x
  5. Cui, Y.Y., D.M. Pandey, E.J. Hahn, and K.Y. Park. 2004. Effect of drought on physiological aspects of crassulacean acid metabolism in Doritaenopsis. Plant Sci. 167:1219-1226. https://doi.org/10.1016/j.plantsci.2004.06.011
  6. Dunford, R.P., S. Griffiths, V. Christodoulou, and D.A. Laurie. 2005. Characterisation of a barley (Hordeum vulgare L.) homologue of the Arabidopsis flowering time regulator GIGANTEA. Theor. Appl. Genet. 110:925-931. https://doi.org/10.1007/s00122-004-1912-5
  7. Fowler, S., K. Lee, H. Onouchi, A. Samach, K. Richardson, B. Morris, G. Coupland, and J. Putterill. 1999. GIGANTEA: a circadian clock-controlled gene that regulates photoperiodic flowering in Arabidopsis and encodes a protein with several possible membrane-spanning domains. EMBO. J. 18:4679-4688. https://doi.org/10.1093/emboj/18.17.4679
  8. Fowler, S. and M.F. Thomashow. 2002. Arabidopsis transcriptome profiling indicates that multiple regulatory pathways are activated during cold acclimation in addition to the CBF cold response pathway. Plant Cell 14:1675-1690. https://doi.org/10.1105/tpc.003483
  9. Hayama, R. and G. Coupland. 2004. The molecular basis of diversity in the photoperiodic flowering responses of Arabidopsis and rice. Plant Physiol. 135:677-684. https://doi.org/10.1104/pp.104.042614
  10. Hayama, R., T. Izawa, and K. Shimamoto. 2002. Isolation of rice genes possibly involved in the photoperiodic control of flowering by a fluorescent differential display method. Plant Cell Physiol. 43:494-504. https://doi.org/10.1093/pcp/pcf059
  11. Hayama, R., S. Yokoi, S. Tamaki, M. Yano, and K. Shimamoto. 2003. Adaptation of photoperiodic control pathways produces short-day flowering in rice. Nature 422:719-722. https://doi.org/10.1038/nature01549
  12. Hecky, J. and K.M. Muller. 2005. Structural perturbation and compensation by directed evolution at physiological temperature leads to thermostabilization of beta-lactamase. Biochemistry 44:12640-12654. https://doi.org/10.1021/bi0501885
  13. Izawa, T., T. Oikawa, S. Tokutomi, K. Okuno, and K. Shimamoto. 2000. Phytochromes confer the photoperiodic control of flowering in rice (a short-day plant). Plant J. 22:391-399. https://doi.org/10.1046/j.1365-313X.2000.00753.x
  14. Jung, J.H., Y.H. Seo, P.J. Seo, J.L. Reyes, J. Yun, N.H. Chua, and C.M. Park. 2007. The GIGANTEA-regulated microRNA172 mediates photoperiodic flowering independent of CONSTANS in Arabidopsis. Plant Cell 19:2736-2748. https://doi.org/10.1105/tpc.107.054528
  15. Kojima, S., Y. Takahashi, Y. Kobayashi, L. Monna, T. Sasaki, T. Araki, and M. Yano. 2002. Hd3a, a rice ortholog of the Arabidopsis FT gene, promotes transition to flowering downstream of Hd1 under short-day conditions. Plant Cell Physiol. 43:1096-1105. https://doi.org/10.1093/pcp/pcf156
  16. Lee, H., S.J. Yoo, J.H. Lee, W. Kim, S.K. Yoo, H. Fitzgerald, J.C. Carrington, and J.H. Ahn. 2010. Genetic framework for flowering-time regulation by ambient temperature-responsive miRNAs in Arabidopsis. Nucleic Acids Res. 38:3081-3093. https://doi.org/10.1093/nar/gkp1240
  17. Levy, Y.Y. and C. Dean. 1998. The transition to flowering. Plant Cell. 10:1973-1990. https://doi.org/10.1105/tpc.10.12.1973
  18. Park, D.H., D.E. Somers, Y.S. Kim, Y.H. Choy, H.K. Lim, M.S. Soh, H.J. Kim, S.A. Kay, and H.G. Nam. 1999. Control of circadian rhythms and photoperiodic flowering by the Arabidopsis GIGANTEA gene. Science 285:1579-1582. https://doi.org/10.1126/science.285.5433.1579
  19. Reeves, P.H. and G. Coupland. 2000. Response of plant development to environment: control of flowering by daylength and temperature. Curr. Opin. Plant Biol. 3:37-42. https://doi.org/10.1016/S1369-5266(99)00041-2
  20. Sothern, R.B., T.S. Tseng, S.L. Orcutt, N.E. Olszewski, and W.L. Koukkari. 2002. GIGANTEA and SPINDLY genes linked to the clock pathway that controls circadian characteristics of transpiration in Arabidopsis. Chronobiol. Int. 19:1005-1022. https://doi.org/10.1081/CBI-120015965
  21. Tranquilli, G. and J. Dubcovsky. 2000. Epistatic interaction between vernalization genes Vrn-Am1 and Vrn-Am2 in diploid wheat. J. Hered. 91:304-306. https://doi.org/10.1093/jhered/91.4.304
  22. Tseng, T.S., P.A. Salome, C.R. McClung, and N.E. Olszewski. 2004. SPINDLY and GIGANTEA interact and act in Arabidopsis thaliana pathways involved in light responses, flowering, and rhythms in cotyledon movements. Plant Cell 16:1550-1563. https://doi.org/10.1105/tpc.019224
  23. Vaz, A.P.A., R.C.L. Figueiredo-Ribeiro, and G.B. Kerbauy. 2004. Photoperiod and temperature effects on in vitro growth and flowering of P. pusilla, an epiphytic orchid. Plant Physiol. Biochem. 42:411-415. https://doi.org/10.1016/j.plaphy.2004.03.008
  24. Yan, L., A. Loukoianov, A. Blechl, G. Tranquilli, W. Ramakrishna, P. SanMiguel, J.L. Bennetzen, V. Echenique, and J. Dubcovsky. 2004. The wheat VRN2 gene is a flowering repressor downregulated by vernalization. Science 303:1640-1644. https://doi.org/10.1126/science.1094305
  25. Yan, L., A. Loukoianov, G. Tranquilli, M. Helguera, T. Fahima, and J. Dubcovsky. 2003. Positional cloning of the wheat vernalization gene VRN1. Proc. Natl. Acad. Sci. USA. 100: 6263-6268. https://doi.org/10.1073/pnas.0937399100
  26. Zhao, X.Y., M.S. Liu, J.R. Li, C.M. Guan, and X.S. Zhang. 2005. The wheat TaGI1, involved in photoperiodic flowering, encodes an Arabidopsis GI ortholog. Plant Mol. Biol. 58:53-64. https://doi.org/10.1007/s11103-005-4162-2