Mycobiology

The Korean Society of Mycology (한국균학회)
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Optimal Media Conditions for the Detection of Extracellular Cellulase Activity in Ganoderma neo-japonicum

  • Jo, Woo-Sik (Department of Agricultural Environment, Gyeongbuk Agricultural Technology Administration) ;
  • Park, Ha-Na (Department of Agricultural Environment, Gyeongbuk Agricultural Technology Administration) ;
  • Cho, Doo-Hyun (Department of Agricultural Environment, Gyeongbuk Agricultural Technology Administration) ;
  • Yoo, Young-Bok (Mushroom Research Division Department of Herbal Crop Research National Institute of Horicultural & Herbal Science, Rural Development Administration) ;
  • Park, Seung-Chun (College of Veterinary Medicine, Kyungpook National University)
  • Received : 2010.12.16
  • Accepted : 2011.05.18
  • Published : 2011.06.30
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Abstract

To determine the optimal media conditions for the detection of the extracellular cellulase activity in Ganoderma neo-japonicum, we varied three media conditions: dye reagent, pH, and temperature. We evaluated the use of four dyes, Congo red, phenol red, remazol brilliant blue, and trypan blue. To observe the effect of pH on the chromogenic reaction, we tested media ranging from 4.5 to 8.0. To research the effect of temperature on the clear zone and the fungus growing zone, we tested temperatures ranging from 15 to $35^{\circ}C$. On the whole, the best protocol called for Ganoderma neo-japonicum transfer onto media containing Congo red with a pH of 7.0, followed by incubation at $25^{\circ}C$ for 5 days. Our results will be useful to researchers who study extracellular enzyme activity in Ganoderma neo-japonicum.

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References

  1. Park WH, Kim TH, Ro IH. Studies on enzymes of the higher fungi of korea (II): identification of cellulolytic enzyme in Lenzites betulina. Kor J Mycol 1986;14:225-9.
  2. Hong JS, Uhm TB, Jung GT, Lee KB. Studies on the enzymes produced by Pleurotus sajor-caju (I). Kor J Mycol 1984;12:59-64.
  3. Hashimoto K. Biochemical studies on the mushroom. Toyo Shokuhin Kenkyusho Kenkyu Hokukusho 1972;10:163.
  4. Lin JM, Lin CC, Chen MF, Ujiie T, Takada A. Radical scavenger and antihepatotoxic activity of Ganoderma formosanum, Ganoderma lucidum and neo-japonicum. J Ethnopharmacol 1995;47:33-41. https://doi.org/10.1016/0378-8741(95)01251-8
  5. Abrahanm L, Hoffman B, Gao Y, Breuil C. Action of Ophiostoma piceae proteinase and lipase on wood nutrients. Can J Microbiol 1998;44:698-701. https://doi.org/10.1139/w98-046
  6. Shin DS, Lee HH, Lim KP, Cho NS, Cho BM. Chemistry of forest product. Seoul: Haeng Mun Sa; 1991. p. 121-2.
  7. Castro GR, Ferrero MA, Mendez BS, Sineriz F. Screening and selection of bacteria with high amylolytic activity. Acta Biotechnol 1993;13:197-201. https://doi.org/10.1002/abio.370130220
  8. Hejgaard J, Gibbons GC. Screening for alpha-amylase in cereals. Improved gel-diffusion assay a dye-labelelled starch substrate. Carlsberg Res Commun 1979;44:21-5. https://doi.org/10.1007/BF02910868
  9. Hyun MW, Yoon JH, Park WH, Kim SH. Detection of cellulolytic activity in Ophiostoma and Leptographium species by chromogenic reaction. Mycobiology 2006;34:108-10. https://doi.org/10.4489/MYCO.2006.34.2.108
  10. Yoon JH, Park JE, Suh DY, Hong SB, Ko SJ, Kim SH. Comparison of dyes for easy detection of extracellular cellulases in fungi. Mycobiology 2007;35:21-4. https://doi.org/10.4489/MYCO.2007.35.1.021

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