Mycobiology

The Korean Society of Mycology (한국균학회)
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Growth and Cultural Characteristics of Ophiocordyceps longissima Collected in Korea

  • Sung, Gi-Ho (Mushroom Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Shrestha, Bhushan (Green Energy Mission/Nepal) ;
  • Han, Sang-Kuk (Division of Forest Biodiversity, Korea National Arboretum) ;
  • Sung, Jae-Mo (Cordyceps Institute of Mushtech)
  • Received : 2011.03.02
  • Accepted : 2011.05.10
  • Published : 2011.06.30
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Abstract

We investigated the effect of nutritional and environmental factors on Ophiocordyceps longissima mycelial growth. The longest colony diameter was observed on Schizophyllum (mushroom) genetics complete medium plus yeast extract, Schizophyllum (mushroom) genetics minimal medium, and Sabouraud dextrose agar (SDA); however, malt-extract yeast-extract agar, SDA plus yeast extract, yeast-extract malt-extract peptone dextrose agar, SDA, oatmeal agar, and potato dextrose agar showed higher mycelia density. A temperature of $25^{\circ}C$ was optimum and 7.0 was the optimum pH for mycelial growth. Colony diameter was similar under light and dark conditions. Maltose and yeast extract showed the highest mycelial growth among carbon and nitrogen sources respectively. The effect of mineral salts was less obvious; however, $K_3PO_4$ showed slightly better growth than that of the other mineral salts tested. Among all nutrition sources tested, complex organic nitrogen sources such as yeast extract, peptone, and tryptone were best for mycelial growth of O. longissima. Ophiocordyceps longissima composite medium, formulated by adding maltose (2% w/v), yeast extract (1% w/v), and $K_3PO_4$ (0.05% w/v) resulted in slightly longer colony diameter. In vitro mycelial O. longissima growth was sustainable and the production of fruiting bodies could be used for commercial purposes in the future.

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References

  1. Kobayasi Y. On the genus Cordyceps and its allies on Cicadidae from Japan. Bull Biogeogr Soc Jpn 1939;9:145-76.
  2. Kobayasi Y, Shimizu D. Monographic studies of Cordyceps 2: Group parasitic on Cicadidae. Bull Natl Sci Mus Tokyo 1963;6:286-314.
  3. Sung JM. The insects-born fungus of Korea in color. Seoul: Kyohak Publishing Co., Ltd.; 1996.
  4. Lee JB, Oh DC. Higher fungi of Cheju-do (1): unrecorded mushrooms. Kor J Mycol 1998;26:538-50.
  5. Li CR, Huang B, Fan MZ, Li ZZ. Cordyceps longissima and its Hirsutella anamorph. J Anhui Agric Univ 1999;26:374-7.
  6. Li CR, Fan MZ, Huang B, Li ZZ. Hirsutella longissima sp. nov., the anamorph of Cordyceps longissima. Mycosystema 2001;20:29-34.
  7. Sung GH, Hywel-Jones NL, Sung JM, Luangsa-ard JJ, Shrestha B, Spatafora JW. Phylogenetic classification of Cordyceps and the clavicipitaceous fungi. Stud Mycol 2007;57:5-59. https://doi.org/10.3114/sim.2007.57.01
  8. Kiho T, Ukai S. Tochukaso (Semitake and others), Cordyceps species. Food Rev Int 1995;11:231-4. https://doi.org/10.1080/87559129509541038
  9. Mizuno T. Medicinal effects and utilization of Cordyceps (Fr.) Link (Ascomycetes) and Isaria Fr. (Mitosporic fungi) Chinese caterpillar fungi, "Tochukaso" (Review). Int J Med Mushrooms 1999;1:251-61. https://doi.org/10.1615/IntJMedMushrooms.v1.i3.80
  10. Kinjo N, Zang M. Morphological and phylogenetic studies on Cordyceps sinensis distributed in southwestern China. Mycoscience 2001;42:567-74. https://doi.org/10.1007/BF02460956
  11. Shrestha B, Zhang W, Zhang Y, Liu X. What is the Chinese caterpillar fungus Ophiocordyceps sinensis (Ophiocordycipitaceae)? Mycology 2010;1:228-36. https://doi.org/10.1080/21501203.2010.536791
  12. Yahagi N. Cultivation of Isaria japonica and inoculation to silkworm. Touchu-Kasou 1985;5:27-30.
  13. Harada Y, Akiyama N, Yamamoto K, Shirota Y. Production of Cordyceps militaris fruit body on artificially inoculated pupae of Mamestra brassicae in the laboratory. Trans Mycol Soc Jpn 1995;36:67-72.
  14. Yamanaka K, Inatomi S. Cultivation of Isaria japonica fruit bodies on mixed plant/insect media. Food Rev Int 1997;13:455-60. https://doi.org/10.1080/87559129709541132
  15. Yamanaka K, Inatomi S, Hanaoka M. Cultivation characteristics of Isaria japonica. Mycoscience 1998;39:43-8. https://doi.org/10.1007/BF02461577
  16. Kana-uchi A, Fukatsu T. Light-induced fruit body formation of an entomogenous fungus Paecilomyces tenuipes. Mycoscience 1999;40:349-51. https://doi.org/10.1007/BF02463879
  17. Chen R, Ichida M. Infection of the silkworm, Bombyx mori, with Cordyceps militaris. J Insect Biotechnol Sericology 2002;71:61-3.
  18. Sato H, Shimazu M. Stromata production for Cordyceps militaris (Clavicipitales: Clavicipitaceae) by injection of hyphal bodies to alternative host insects. Appl Entomol Zool 2002;37:85-92. https://doi.org/10.1303/aez.2002.85
  19. Yahagi N, Yahagi R, Takano F, Fushiya S, Tanaka T, Murakami K, Ohta T. Growth of ascocarps from cultured Cordyceps militaris (L.:Fr.) Fr. and Cordyceps formicarum Kobayasi in an agar medium. Nippon Kingakkai Kaiho 2004;45:15-9.
  20. Li CR, Nam SH, Geng DG, Fan MZ, Li ZZ. Artificial culture of seventeen Cordyceps spp. Mycosystema 2006;25:639-45.
  21. Lee JO, Shrestha B, Kim TW, Sung GH, Sung JM. Stable formation of fruiting body in Cordyceps bassiana. Mycobiology 2007;35:230-4. https://doi.org/10.4489/MYCO.2007.35.4.230
  22. Lee JO, Shrestha B, Sung GH, Han SK, Kim TW, Sung JM. Cultural characteristics and fruiting body production in Cordyceps bassiana. Mycobiology 2010;38:118-21. https://doi.org/10.4489/MYCO.2010.38.2.118
  23. Kim SY, Shrestha B, Sung GH, Han SK, Sung JM. Optimum conditions for artificial fruiting body formation of Cordyceps cardinalis. Mycobiology 2010;38:133-6. https://doi.org/10.4489/MYCO.2010.38.2.133
  24. Sung GH, Shrestha B, Park KB, Sung JM. Cultural characteristics of Shimizuomyces paradoxus collected from Korea. Mycobiology 2010;38:189-94. https://doi.org/10.4489/MYCO.2010.38.3.189
  25. Shrestha B, Lee WH, Han SK, Sung JM. Observations on some of the mycelial growth and pigmentation characteristics of Cordyceps militaris isolates. Mycobiology 2006;34:83-91. https://doi.org/10.4489/MYCO.2006.34.2.083
  26. Sung GH, Shrestha B, Han SK, Kim SY, Sung JM. Growth and cultural characteristics of Cordyceps cardinalis collected from Korea. Mycobiology 2010;38:274-81. https://doi.org/10.4489/MYCO.2010.38.4.274
  27. Sung GH, Shrestha B, Han SK, Sung JM. Cultural characteristics of Ophiocordyceps heteropoda collected from Korea. Mycobiology 2011;39:1-6. https://doi.org/10.4489/MYCO.2011.39.1.001
  28. Sasaki F, Miyamoto T, Tamai Y, Yajima T. Optimum temperature and pH for mycelial growth of Cordyceps nutans Pat. (Ascomycetes). Int J Med Mushrooms 2005;7:301-4. https://doi.org/10.1615/IntJMedMushr.v7.i12.270
  29. Sung GH, Shrestha B, Sung JM. Characteristics of Metacordyceps yongmunensis, a new species from Korea. Mycobiology 2010;38:171-5. https://doi.org/10.4489/MYCO.2010.38.3.171

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