한국축산식품학회지 (Food Science of Animal Resources)

  • 제31권3호
  • /
  • Pages.356-365
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  • 2011
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  • 2636-0772(pISSN)
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  • 2636-0780(eISSN)
한국축산식품학회 (Korean Society for Food Science of Animal Resources)
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Porcine Fatty Acid Synthase Gene Polymorphisms Are Associated with Meat Quality and Fatty Acid Composition

  • Kim, Sang-Wook (Department of Animal Science, Chungbuk National University) ;
  • Choi, Yang-Il (Department of Animal Science, Chungbuk National University) ;
  • Choi, Jung-Suck (Department of Animal Science, Chungbuk National University) ;
  • Kim, Jong-Joo (School of Biotechnology, Yeungnam University) ;
  • Choi, Bong-Hwan (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science) ;
  • Kim, Tae-Hun (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science) ;
  • Kim, Kwan-Suk (Department of Animal Science, Chungbuk National University)
  • 투고 : 2011.02.09
  • 심사 : 2011.04.14
  • 발행 : 2011.06.30
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초록

We assessed the effects of single-nucleotide polymorphisms (SNPs) within the porcine fatty acid synthase (FASN) gene regarding meat quality and fatty acid composition in two pig populations: Korean native pigs (KNP) were crossed with Yorkshire (YS) $F_2$, and KNP were crossed with Landrace (LR) $F_2$. Direct DNA sequencing using eight KNP and eight YS pigs revealed two SNPs: c.265C>T (silent) in exon 4 and c.6545A>C (Asn${\rightarrow}$His) in exon 39. The frequency of the two SNPs was analyzed using the polymerase chain reaction-restriction fragment length polymorphism method in seven pig breeds and their association with meat quality traits and fatty acid composition was studied. In the $KNP{\times}YSF_2$ population, both SNPs were significantly associated with the level of monounsaturated fatty acids, including palmitoleic (C16:1) and oleic acid (C18:1) (p<0.005). c.6545A>C was associated with intramuscular fat content in both populations. Our results indicate that variations in c.265C>T and c.6545A>C of the pig FASN can be used to select animals with better fatty acid composition and meat quality. Moreover, KNP was a useful breed for identifying genetic factors affecting meat quality and fatty acid composition and for producing high quality pork.

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참고문헌

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  3. Evaluation of Three Candidate Genes Affecting Fatty Acid Composition in Pigs vol.32, pp.1, 2012, https://doi.org/10.5851/kosfa.2012.32.1.6
  4. Disentangling Two QTL on Porcine Chromosome 12 for Backfat Fatty Acid Composition vol.24, pp.3, 2013, https://doi.org/10.1080/10495398.2012.763130
  5. Relationships between Single Nucleotide Polymorphism Markers and Meat Quality Traits of Duroc Breeding Stocks in Korea vol.29, pp.9, 2016, https://doi.org/10.5713/ajas.16.0158
  6. Identification of Molecular Mechanisms Related to Pig Fatness at the Transcriptome and miRNAome Levels vol.11, pp.6, 2011, https://doi.org/10.3390/genes11060600