Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effect of Dioscorea Aimadoimo on Anti-aging and Skin Moisture Capacity

마의 항노화 및 피부 보습 효과

  • Kim, Dae-Sung (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Jeon, Byoung-Kook (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Mun, Yeun-Ja (Department of Anatomy, College of Oriental Medicine, Wonkwang University) ;
  • Kim, Yeong-Mok (Department of Oriental Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Lee, Young-Eun (Department of Food and Nutrition, College of Environmental Resources, Wonkwang University) ;
  • Woo, Won-Hong (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 김대성 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 전병국 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 문연자 (원광대학교 한의과대학 해부학교실) ;
  • 김영목 (원광대학교 한의과대학 병리학교실) ;
  • 이영은 (원광대학교 생활과학대학 식품영양학과) ;
  • 우원홍 (원광대학교 한의학전문대학원 한약자원개발학과)
  • Received : 2011.04.26
  • Accepted : 2011.06.08
  • Published : 2011.06.25
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Abstract

This study evaluated the effect of extraction conditions of yam (Dioscorea aimadoimo) on antioxidant, moisturizing, collagenase activity, proliferation, and migration. Yam has been recognized as a healthy food due to its various biological activities, such as anti-obesity, anti-constipation, anti-mutagenic activities, as well as its ability to decrease blood glucose and cholesterol levels. Electron donating ability of high temperature ethanol extract of Dioscorea aimadoimo (HDA) had shown 70.6% at 400 mg/ml, and low temperature ethanol extract of Dioscorea aimadoimo (LDA) had shown 40% at 400 mg/ml. SOD-like activities of LDA and HDA were 23% and 34% at 400 mg/ml respectively. LDA significantly reduced the activity of collagenase in a dose-dependent manner, which was higher than HDA. The water contents in LDA-treated skin and HDA-treated skin were increased by 45.63% and 38.65% than the placebo cream respectively. The cellular proliferation of human dermal fibroblast neonatal (HDFn) was evaluated by MTT and cell migration assay. Compared to control, the cell proliferation was elevated to 109.7% and 114% by the treatment of LDA and HDA respectively at the concentration of 200 mg/ml. In addition, LDA and HDA were induced cell migration in HDFn. Our study suggests that LDA and HDA should be a very useful cosmetic ingredient, as anti-aging and skin moisturizer.

Keywords

References

  1. Ahn, J.H., Son, K.H., Sohn, H.Y., Kwon, S.T. In vitro coulture of adventitious roots from Dioscorea nipponica Makino for the production of steroidal saponins. Korean J Plant Biotechnol. 32: 317-223, 2005.
  2. 김지인, 장한수, 김종식, 손호용. 마(Dioscorea batatas Decne)의 항균, 항산화 및 항혈전 활성 평가. Kor J Microbiol Biotechnol 37(2):133-139, 2009.
  3. Kum, E.J., Park, S.J., Lee, B.H., Kim, J.S., Son, K.H., Sohn, H.Y. Antifungal activity of phenanthrene derivatives from aerial bulbils of Dioscorea batatas Decne. J Life Sci 16:647-652, 2006. https://doi.org/10.5352/JLS.2006.16.4.647
  4. Kim, J.I., Jang, H.S., Kim, J.S., Sohn, H.Y. Evaluation of antimicrobial, antithrombin, and antioxidant activity of Dioscorea batatas Decne. Korean J Microbiol Biotechnol. 37:133-139, 2009.
  5. Kwon, C.S., Sohn, H.Y., Kim, S.H., Kim, J.H., Son, K.H., Lee, J.S., Lim, J.K., Kim, J.S. Anti-obesity effect of Dioscorea nipponica Makino with lipase-inhibitory activity in rodents. Biosci Biotechnol Biochem 67: 1451-1456, 2003. https://doi.org/10.1271/bbb.67.1451
  6. Son, I.S., Kim, J.I., Sohn, H.Y., Son, K.H., Kim, J.S., Kwon, C.S. Antioxidative and hypolipidemic effects of diosgenin, a steroidal saponin of yam (Dioscorea spp), on hing-cholesterol fed rats. Biosci Biotechnol Biochem 71:3063-3071, 2007. https://doi.org/10.1271/bbb.70472
  7. Kang, T.H., Choi, S.Z., Lee, T.H., Son, M.W., Kim, S.Y. Characteristics of antidiabetic effect of Dioscorea rhizoma (1) - Hypoglycemic effect. Korean J Food Nutr 21: 425-429, 2008.
  8. Kang, T.H., Choi, S.Z., Lee, T.H., Son, M.W., Kim, S.Y. Characteristics of antidiabetic effect of Dioscorea rhizoma (2)-prevention of diabetic neuropathy by NGF induction. Korean J Food Nutr 21: 430-435, 2008.
  9. Kim, M.W. Effects of H2O-fraction of Dioscorea Japonica Thunb and selenium on lipid peroxidation in streptoxotocin-induced diabetic rats. Korean J Soc Food Cookery Sci. 17: 344-352, 2001.
  10. Kwon, E.G., Choe, E.M., Gu, S.J. Effects of mucilage from yam (Dioscorea btatas DECNE) on blood glucose and lipid composition in alloxan-induced diabetic mice. Korean J Food Sci Technol 33: 795-801, 2001.
  11. Lee, I.S., Chung, S.Y., Shim, C.S., Koo, S.J. Inhibitory effects of yam (Dioscorea btatas DECNE) extracts on the mutagenicity. Korean J Soc Food Sci 11: 351-355, 1995.
  12. 金昌玟, 辛民敎, 安德均, 李京淳外. 中藥大辭典. 서울, 鼎談出版社, pp 20671-2071, 1997.
  13. Pryored, W.A. Free radicals in biology. Academic press. 1084;VI:371.
  14. Makrantonaki, E., Zouboulis, C.C. Molecular mechani는 of skin aging: state of the art. Ann N Y Acad Sci 1119: 40-50, 2007. https://doi.org/10.1196/annals.1404.027
  15. Seo, M.Y., Chung, S.Y., Choi, W.K., Seo, Y.K., Jung, J.H., Park, J.M., Seo, M.J., Park, J.K., Kim, J.W., Park, C.S. Anti-aging effect of rice wine in cultured human fibroblasts and keratinocytes. J Biosci Bioeng 107: 266-271, 2009. https://doi.org/10.1016/j.jbiosc.2008.11.016
  16. Tanaka, K., Hasegawa, J., Asamitsu, K., Okamoto, T. Prevention of the ultraviolet B-mediated skin photoaging by a nuclear factor kappaB inhibitor, parthenolide. J Pharmacol Exp Ther 315: 624-630, 2005. https://doi.org/10.1124/jpet.105.088674
  17. Imodawa, G. Recent advaces in characterizing biological mechanisms underlying UV-induced wrinkles: a pivotal role of fibroblast-derived elastase. Arch dermatol Res 300:7-20, 2008. https://doi.org/10.1007/s00403-007-0798-x
  18. Kim, E.J., Kim, M.K., Jin, X.J., Oh, J.H., Kim, J.E., Chung, J.H. Skin aging and photoaging alter fatty acids composition, including 11,14,17-eicosatrienoic acid, in the epidermis of human skin. J Korean Med Sci 25: 980-983, 2010. https://doi.org/10.3346/jkms.2010.25.6.980
  19. Jacobi, O.K. About the mechanism of moisture regulation in the horny layer of skin, Proc Sci Sec Toilet Goods Assoc 31: 22, 1959.
  20. Blois, M.S. Antioxidant determination by the use of a stable free radical. Nature 181: 1199-1200, 1958. https://doi.org/10.1038/1811199a0
  21. Marklund, S., Marklund, G. Involvement of superoxide anion radical in the oxidation of pyrogallol and convenient assay for superoxide dismutase. Eur J Biochem 47: 468-474, 1975.
  22. Mosmann, T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65: 55-63, 1983. https://doi.org/10.1016/0022-1759(83)90303-4
  23. Suzanne, E.G.F., James, V., Subhash, C.D., Sewon, K., Gary, J.F., John, J. Collagen degradation in aged/photodamaged sin in vivo and after exposure to matrix metalloproteinase-1 in vitro. J Invest Dermatol 120: 842-848, 2003. https://doi.org/10.1046/j.1523-1747.2003.12148.x
  24. Nice, D.J., Robinson, D.S., Holden, M.A. Characterization of a heat stable antioxidant co purifiec with the supersixde dismutase activity from dried peas, Food Chem 52:393-397, 1995. https://doi.org/10.1016/0308-8146(95)93288-3
  25. Kim, S.J., Han, D., Moon, K.D., Rhee, J.S. Measurement of superoxide dismutase-like activity of natural antioxidant. Biosci Biochem 59: 822-826, 1995. https://doi.org/10.1271/bbb.59.822
  26. Yaar, M., Gilchrest, B.A. Aging versus photoaging: Phostulated Mechani는 and effectors. J Invest Dermatol Symp Proc 3: 47-51, 1998.
  27. Flynn, E.C., Petros, J., Clark, R.E., Viehman, G.E. Dry skin and moisturizers. Clin dermatol 19: 387-392, 2001.
  28. Care, K.R.G.. Advances in wound care. Seoul: Korea Medical Book Publisher. 2002.
  29. Kubo, K.K.Y. Spongy matrix of hyaluronic acid and collagen as a cultured dermal substitute: evaluation in an animal test. J Artif Organs 6: 64-70, 2003. https://doi.org/10.1007/s100470300010