The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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Effects of Saengmaekcheongpye-eum on LPS-Induced COPD Model

LPS로 유발된 만성폐쇄성폐질환에 대한 생맥청폐음(生脈淸肺飮)의 영향

  • Kim, Yong (Division of Respiratory System, Dept. of Internal Medicine, College of Oriental Medicine, Dae-jeon University) ;
  • Yang, Su-Young (Division of Respiratory System, Dept. of Internal Medicine, College of Oriental Medicine, Dae-jeon University) ;
  • Kim, Min-Hee (Dept. of Neurophysiology, College of Oriental Medicine, Dae-jeon University) ;
  • NamGung, Uk (Dept. of Neurophysiology, College of Oriental Medicine, Dae-jeon University) ;
  • Park, Yang-Chun (Division of Respiratory System, Dept. of Internal Medicine, College of Oriental Medicine, Dae-jeon University)
  • 김용 (대전대학교 한의과대학 폐계내과학교실) ;
  • 양수영 (대전대학교 한의과대학 폐계내과학교실) ;
  • 김민희 (대전대학교 한의과대학 신경생리학교실) ;
  • 남궁욱 (대전대학교 한의과대학 신경생리학교실) ;
  • 박양춘 (대전대학교 한의과대학 폐계내과학교실)
  • Published : 2011.06.30
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Abstract

Objectives : This study aimed to evaluate the effects of Saengmaekcheongpye-eum (SCE) on a LPS-induced COPD (chronic obstructive pulmonary disease) model. Materials and Methods : The extract of SCE was treated to A549 cells and and LPS-induced COPD mouse model. Then, various parameters such as cell-based cyto-protective activity and histopathological finding were analyzed. Results : SCE showed a protective effect on LPS-induced cytotoxicity in A549 cells. This effect was correlated with analysis for caspase 3 levels, elastin contents, protein levels of cyclin B1, Cdc2, and phospho-Erk1/2, and gene expression of TNF-${\alpha}$ and IL-$1{\beta}$ in A549 cells. SCE treatment also revealed a protective effect on LPS-induced lung injury in COPD mouse model. This effect was evidenced via histopathological findings including immunofluorescence stains against elastin and caspase 3, and protein levels of cyclin B1, Cdc2, and Erk1/2 in lung tissue. Conclusions : These data suggest that SCE has pharmaceutical properties on lung injury. This study thus provides scientific evidence for the efficacy of SCE for clinical application to patients with COPD.

Keywords

References

  1. 유철규. 만성폐쇄성폐질환의 병인과 병태생리. 대한내과학회지 2009;77:383-400.
  2. Viegi G, Pistelli F, Sherrill DL, Maio S, Baldacci S, Carrozzi L. Definition, epidemiology and natural history of COPD. Eur Respir J 2007;30:993-1013. https://doi.org/10.1183/09031936.00082507
  3. Celli BR. ATS standards for the optimal management of chronic obstructive pulmonary disease. Respirology 1997;2 Suppl 1:S1-4.
  4. Urbano FL, Pascual RM. Contemporary issues in the care of patients with chronic obstructive pulmonary disease. J Manag Care Pharm 2005;11(5SupplA):S2-13.
  5. 장준. 만성 폐쇄성 폐질환의 약물치료. 대한결핵 및 호흡기학회지 2005;59(3):231-42.
  6. Lee DJ. The effects of Lonicera japonica on the murine model of Chronic obstructive pulmonary disease. 학위논문(박사). 경희대학교대학원; 2009.
  7. 김기태, 고흥. 柴梗半夏湯이 LPS로 유발된 급성 폐손상에 대한 영향. 동의생리병리학회지 2009;26:1349-57.
  8. 오지석, 박양춘. 加味八味丸의 elastase 유도성 A549 세포사멸에 대한 보호효과. 대한한의학회지 2010;31(2):137-48.
  9. 윤종만, 박양춘. Elastase 매개성 폐조직 손상에 대한 선폐정천탕의 보호효과. 대한한방내과학회지 2010;31(1):84-101.
  10. 전국한의과대학 폐계내과학교실. 동의폐계내과학. 서울: 한문화사; 2002, p. 335-46.
  11. 허준. 동의보감. 서울: 남산당; 1986, p. 482.
  12. Torres A, Dorca J, Zalacaín R, Bello S, El-Ebiary M, Molinos L, et al. Community-acquired pneumonia in chronic obstructive pulmonary disease: a Spanish multicenter study. Am J Respir Crit Care Med 1996;154:1456-61. https://doi.org/10.1164/ajrccm.154.5.8912764
  13. Soler-Cataluna JJ, Martinex-Garcia MA, Roman Sanchez P, Salcedo E, Navarro M, Ochando R. Severe acute exacerbations and mortality in patients with chronic obstructive pulmonary disease. Thorax 2005;60:925-31. https://doi.org/10.1136/thx.2005.040527
  14. The National Lung Health Education Program (NLHEP). Strategies in preserving lung health and preventing COPD and associated diseases. Chest 1998;113:123S-63S. https://doi.org/10.1378/chest.113.2_Supplement.123S
  15. Piquet J, Maurer C, Barbieux H, Chavaillon JM, Debieuvre D, Lebas FX, et al. Acute exacerbations of COPD admitted to hospital: predictive factors of 3 year mortality. Rev Mal Respir 2007;24:909-16. https://doi.org/10.1016/S0761-8425(07)91397-2
  16. Sethi S, Murphy TF. Infection in the pathogenesis and course of chronic obstructive pulmonary disease. N Engl J Med 2008;359:2355-65. https://doi.org/10.1056/NEJMra0800353
  17. Vadas P, Browning J, Edelson J, Pruzanski W. extracellular phospholipase A2 expression and inflammation. J Lipid Mediat 1993;8:1-8.
  18. 김선영, 김창환, 김태규, 김희제, 서영훈, 성정석, 등. 면역학 제2판. 서울: 라이프사이언스; 2006, p. 223-73.
  19. Cosio MG, Cosio Piqueras MG. Pathology of emphysema in chronic obstructive pulmonary disease. Monaldi Arch Chest Dis 2000;55(2): 124-9.
  20. Tetley TD. Macrophages and the pathogenesis of COPD. Chest 2002;121(5 Suppl):S156-9. https://doi.org/10.1378/chest.121.5_suppl.156S
  21. Mak JC, Ho SP, Yu WC, Choo KL, Chu CM, Yew WW, et al. Polymorphisms and functional activity in superoxide dismutase and catalase genes in smokers with COPD. Eur Respir J 2007;30(4):684-90. https://doi.org/10.1183/09031936.00015207
  22. Meduri GU, Headley S, Kohler G, Stentz F, Tolley E, Umberger R, et al. Persistent elevation of inflammatory cytokines predicts a poor outcome in ARDS. Plasma IL-1 beta and IL-6 levels are consistent and efficient predictors of outcome over time. Chest 1995;107:1062-73. https://doi.org/10.1378/chest.107.4.1062
  23. Martin TR. Recognition of bacterial endotoxin in the lungs. Am J Respir Cell mol Biol 2000;23(2):128-32. https://doi.org/10.1165/ajrcmb.23.2.f189
  24. 전국한의과대학 본초학교실. 本草學. 서울: 영림사; 1994, p. 420-623.
  25. Gross D, Shenkman Z, Bleiberg B, Dayan M, Gittelson M, Efrat R. Ginseng improves pulmonary functions and exercise capacity in patients with COPD. Monaldi Arch Chest Dis 2002;57(5-6):242-6.
  26. Xie YC, Dong XW, Wu XM, Yan XF, Xie QM. Inhibitory effects of flavonoids extracted from licorice on lipopolysaccharide-induced acute pulmonary inflammation in mice. Int Immunopharmacol 2009;9(2):194-200. https://doi.org/10.1016/j.intimp.2008.11.004
  27. Guo R, Pittler MH, Ernst E. Herbal medicines for the treatment of COPD: a systematic review. Eur Respir J 2006;28(2):330-8. https://doi.org/10.1183/09031936.06.00119905
  28. Nakajoh M, Fukushima T, Suzuki T, Yamaya M, Nakayama K, Sekizawa K, et al. Retinoic acid inhibits elastase-induced injury in human lung epithelial cell lines. Am J Respir Cell Mol Biol 2003;28:296-304. https://doi.org/10.1165/rcmb.4845
  29. Fiorenza D, Viglio S, Lupi A, Baccheschi J,Tinelli C, Trisolini R, et al. Urinary desmosine excretion in acute exacerbations of COPD: a preliminary report. Respir Med 2002;96(2):110-4. https://doi.org/10.1053/rmed.2001.1224
  30. Cocci F, Miniati M, Monti S, Cavarra E, Gambelli F, Battolla L, et al. Urinary desmosine excretion is inversely correlated with the extent of emphysema in patients with chronic obstructive pulmonary disease. Int J Biochem Cell Biol 2002;34(6):594-604. https://doi.org/10.1016/S1357-2725(02)00015-8
  31. Yu SA, Kim SC, Jo MS, Suh DS, Kim KH, Yoon MS. Anti-proliferation effect in epithelial ovarian cancer cells by MEK inhibitor AZD6244. Korean J Obstetrics and Gynecology 2010;53:243-53. https://doi.org/10.5468/kjog.2010.53.3.243
  32. Yoon S, Seger R. The extracellular signal-regulated kinase: multiple substrates regulate diverse cellular functions. Growth Factors 2006;24:21-44. https://doi.org/10.1080/02699050500284218
  33. Nigg EA. The substrates of the cdc2 kinase. Semin Cell Biol 1991;2:261-70.
  34. Nigg EA, Krek W, Peter M. Vertebrate cdc2 kinase: its regulation by phosphorylation and its mitotic targets. Cold Spring Harb Symp Quant Biol 1991;56:539-47. https://doi.org/10.1101/SQB.1991.056.01.061
  35. Enoch T, Nurse P. Coupling M phase and S phase: controls maintaining the dependence of mitosis on chromosome replication. Cell 1991;65:921-3. https://doi.org/10.1016/0092-8674(91)90542-7
  36. Park WY, Goodman RB, Steinberg KP, Ruzinski JT, Radella F 2nd, Park DR, et al. Cytokine balance in the lungs of patients with acute respiratory distress syndrome. Am J Respir Crit Care Med 2001;164:1896-903. https://doi.org/10.1164/ajrccm.164.10.2104013
  37. Keane MP, Strieter RM. The importance of balanced pro-inflammatory and anti-inflammatory mechanism in diffuse lung disease. Respir Res 202;3:5. https://doi.org/10.1186/rr177
  38. Kolb M, Margetts PJ, Anthony DC, Pitossi F, Gauldie J. Transient expression of IL-$1{\beta}$ induces acute lung injury and chronic repair leading to pulmonary fibrosis. J Clin Invest 2001;107:1529-36. https://doi.org/10.1172/JCI12568