대한본초학회지 (The Korea Journal of Herbology)

  • 제26권1호
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  • Pages.87-96
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  • 2011
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  • 1229-1765(pISSN)
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  • 2288-7199(eISSN)
대한본초학회 (The Korea Association of Herbology)
권호 표지
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양제엽(羊蹄葉) 메탄올 추출물의 항산화 및 항염증 효과

Anti-oxidative and anti-inflammatory effects of aerial parts of Rumex japonicus Houtt. in RAW 264.7 cells

  • 조현진 (동국대학교 한의과대학 방제학교실) ;
  • 윤현정 (동국대학교 한의과대학 방제학교실) ;
  • 박선동 (동국대학교 한의과대학 방제학교실)
  • Cho, Hyun-Jin (Dept. of Prescriptionology, College of Oriental Medicine, Dongguk University) ;
  • Yun, Hyun-Jeong (Dept. of Prescriptionology, College of Oriental Medicine, Dongguk University) ;
  • Park, Sun-Dong (Dept. of Prescriptionology, College of Oriental Medicine, Dongguk University)
  • 투고 : 2011.02.07
  • 심사 : 2011.03.10
  • 발행 : 2011.03.30
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초록

Objectives : The aerial parts of Rumex japonicus Houtt. (RF) is used by traditional clinics to treat parasite infection in East asia. This study aims a verification of anti-oxidative and anti-inflammatory effects of RF methanol extract. Methods : Anti-oxidative effects of RF were measured by scavenging activities of DPPH, superoxide, nitric oxide (NO) and peroxynitrite radicals. And also scavenging activities of anti-oxidation in lipopolysaccharide (LPS)-treated RAW 264.7 cells were measured. The inhibitory effects against the production of inflammatory mediators including NO, prostaglandin $E_2$ ($PGE_2$), tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), interleukin-$1{\beta}$ (IL-$1{\beta}$), IL-6, inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and the translocation of nuclear factor (NF)-${\kappa}B$ in LPS-stimulated RAW 264.7 cells by RF were tested. Results : RF scavenged DPPH, superoxide, NO and peroxynitrite radicals, and RF (at $200{\mu}g/m{\ell}$) reduced the inflammatory mediators definitely. Conclusions : These results indicate that RF may be a potential drug source for oxidative stress related inflammatory diseases.

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참고문헌

  1. Scrivo R, Vasile M, Bartosiewicz I, Valesini G. Inflammation as "common soil" of the multifactorial diseases. Autoimmun Rev. 2010 ; Epub ahead of print.
  2. Xie W, Du L. Diabetes is an inflammatory disease : evidences from traditional Chinese Medicines. Diabetes Obes Metab. 2010 ; Epub ahead of print.
  3. Gosslau A, Li S, Ho CT, Chen KY, Rawson NE. The importance of natural product characterization in studies of their anti-inflammatory activity. Mol Nutr Food Res. 2011 ; 55(1) : 74-82. https://doi.org/10.1002/mnfr.201000455
  4. Olson N, van der Vliet A. Interactions between nitric oxide and hypoxia-inducible factor signaling pathways in inflammatory disease. Nitric Oxide. 2011 ; Epub ahead of print
  5. Marchesi VT. Alzheimer's dementia begins as a disease of small blood vessels, damaged by oxidative-induced inflammation and dysregulated amyloid metabolism : implications for early detection and therapy. FASEB J. 2011 ; 25(1) : 5-13. https://doi.org/10.1096/fj.11-0102ufm
  6. Mendoza-Nunez VM, Rosado-Perez J, Santiago-Osorio E, Ortiz R, Sanchez-Rodriguez MA, Galvan-Duarte RE. Aging Linked to Type 2 Diabetes Increases Oxidative Stress and Chronic Inflammation. Rejuvenation Res. 2011 ; Epub ahead of print.
  7. Elzaawely AA, Xuan TD, Tawata S. Antioxidant and antibacterial activities of Rumex japonicus HOUTT. Aerial parts. Biol Pharm Bull. 2005 ; 28(12) : 2225-30. https://doi.org/10.1248/bpb.28.2225
  8. Jang DS, Kim JM, Kim J, Yoo JL, Kim YS, Kim JS. Effects of compounds isolated from the fruits of Rumex japonicus on the protein glycation. Chem Biodivers. 2008 ; 5(12) : 2718-23. https://doi.org/10.1002/cbdv.200890226
  9. Elzaawely AA, Xuan TD, Tawata S. Antioxidant and antibacterial activities of Rumex japonicus HOUTT. Aerial parts. Biol Pharm Bull. 2005 ; 28(12) : 2225-30. https://doi.org/10.1248/bpb.28.2225
  10. Park YK. Studies on the anti-oxidative effects of Rumexis Radix. Kor. J. Herbology. 2003 ; 18(4) : 269-277.
  11. Cho KH, Kim HS. A study of antifungal activity with Rumex Japonicus Houttuyn. The Korean journal of Dermatology. 1980 ; 18(5) : 383-389.
  12. Lee HS, Kim SK, Han JB, Choi HM, Park JH, Kim EC, Choi MS, An HJ, Um JY, Kim HM, Min BI. Inhibitory effects of Rumex japonicus Houtt. on the development of atopic dermatitis-like skin lesions in NC/Nga mice. Br J Dermatol. 2006 ; 155(1) : 33-8. https://doi.org/10.1111/j.1365-2133.2006.07303.x
  13. Kumari A, Yadav SK, Pakade YB, Kumar V, Singh B, Chaudhary A, Yadav SC. Nanoencapsulation and characterization of Albizia chinensis isolated antioxidant quercitrin on PLA nanoparticles. Colloids Surf B Biointerfaces. 2011 ; 82(1) : 224-32. https://doi.org/10.1016/j.colsurfb.2010.08.046
  14. Jang DS, Kim JM, Kim JH, Kim JS. 24-nor-Ursane type triterpenoids from the stems of Rumex japonicus. Chem Pharm Bull (Tokyo). 2005 ; 53(12) : 1594-6. https://doi.org/10.1248/cpb.53.1594
  15. Koyama J, Morita I, Kawanishi K, Tagahara K, Kobayashi N. Capillary electrophoresis for simultaneous determination of emodin, chrysophanol, and their 8-beta-D-glucosides. Chem Pharm Bull (Tokyo). 2003 ; 51(4) : 418-20. https://doi.org/10.1248/cpb.51.418
  16. Gyamfi MA, Yonamine M, Aniya Y. Free-radical scavenging action of medicinal herbs from Ghana. Gen Pharmacol. 1999 ; 32 : 661-7. https://doi.org/10.1016/S0306-3623(98)00238-9
  17. Gotoh N, Niki E. Rates of interactions of superoxide with vitamin E, vitamin C and related compounds as measured by chemiluminescence. Biochim Biophys Acta. 1992 ; 1115 : 201-7. https://doi.org/10.1016/0304-4165(92)90054-X
  18. Nagata N, Momose K, Ishida Y. Inhibitory effects of catecholamines and anti-oxidants on the fluorescence reaction of 4,5-diaminofluorescein, DAF-2, a novel indicator of nitric oxide. J Biochem. 1999 ; 125 : 658-61. https://doi.org/10.1093/oxfordjournals.jbchem.a022333
  19. Kooy, N.W., Royall, J.A. Ischiropulos, H. and Beckman, J.S. Peroxynitrite-mediated oxidation of dihydrorhodamine 123. Free Radical Biol. Med. 1994 ; 16 : 149-56. https://doi.org/10.1016/0891-5849(94)90138-4
  20. Rong Y, Geng Z, Lau BH. Ginko biloba attenuates oxidative stress in macrophages and endothelial cells. Free Radic Biol Med. 1996 ; 20 : 121-7. https://doi.org/10.1016/0891-5849(95)02016-0
  21. Wang H, Joseph JA. Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate reader. Free Radic Biol Med. 1999 ; 27 : 612-6. https://doi.org/10.1016/S0891-5849(99)00107-0
  22. Wang S, Chen Y, He D, He L, Yang Y, Chen J, Wang X. Inhibition of vascular smooth muscle cell proliferation by serum from rats treated orally with Gastrodia and Uncaria decoction, a traditional Chinese formulation. J Ethnopharmacol. 2007 ; 114(3) : 458-62. https://doi.org/10.1016/j.jep.2007.08.039
  23. Yoon SB, Lee YJ, Park SK, Kim HC, Bae H, Kim HM, Ko SG, Choi HY, Oh MS, Park W. Anti-inflammatory effects of Scutellaria baicalensis water extract on LPS-activated RAW 264.7 macrophages. J Ethnopharmacol. 2009 ; 125(2) : 286-90. https://doi.org/10.1016/j.jep.2009.06.027
  24. Liu L, Guo Z, Lv Z, Sun Y, Cao W, Zhang R, Liu Z, Li C, Cao S, Mei Q. The beneficial effect of Rheum tanguticum polysaccharide on protecting against diarrhea, colonic inflammation and ulceration in rats with TNBS-induced colitis : the role of macrophage mannose receptor in inflammation and immune response. Int Immunopharmacol. 2008 ; 8(11) : 1481-92. https://doi.org/10.1016/j.intimp.2008.04.013
  25. Kim JM, Jung HA, Choi JS, Min BS, Lee NG. Comparative analysis of the anti-inflammatory activity of Huang-lian extracts in lipopolysaccharide-stimulated RAW264.7 murine macrophage-like cells using oligonucleotide microarrays. Arch Pharm Res. 2010 ; 33(8) : 1149-57. https://doi.org/10.1007/s12272-010-0803-3
  26. Heo J. Dongeubogam. Seoul, Bubinsa. 1999 ; p1955.
  27. Moon JK, Shibamoto T. Antioxidant assays for plant and food components. J Agric Food Chem. 2009 ; 57(5) : 1655-66. https://doi.org/10.1021/jf803537k
  28. Kryston TB, Georgiev AB, Pissis P, Georgakilas AG. Role of Oxidative stress and DNA Damage in Human Carcinogenesis. Mutat Res. 2011 ; Epub ahead of print.
  29. Cai H, Harrison DG. Endothelial dysfunction in cardiovascular diseases : the role of oxidant stress. Circ Res. 2000 ; 87(10) : 840-4. https://doi.org/10.1161/01.RES.87.10.840
  30. Moon JK, Shibamoto T. Antioxidant assays for plant and food components. J Agric Food Chem. 2009 ; 57(5) : 1655-66. https://doi.org/10.1021/jf803537k
  31. Giacco F, Brownlee M. Oxidative stress and diabetic complications. Circ Res. 2010 ; 107(9) : 1058-70. https://doi.org/10.1161/CIRCRESAHA.110.223545
  32. Salvemini D, Doyle TM, Cuzzocrea S. Superoxide, peroxynitrite and oxidative/nitrative stress in inflammation. Biochem Soc Trans. 2006 ; 34(5) : 965-70. https://doi.org/10.1042/BST0340965
  33. Lonkar P, Dedon PC. Reactive species and DNA damage in chronic inflammation : Reconciling chemical mechanisms and biological fates. Int J Cancer. 2010 ; Epub ahead of print.
  34. Diatchuk V, Lotan O, Koshkin V, Wikstroem P, Pick E. Inhibition of NADPH oxidase activation by 4-(2-aminoethyl)-benzenesulfonyl fluoride and related compounds. J Biol Chem. 1997 ; 272(20) : 13292-301. https://doi.org/10.1074/jbc.272.20.13292
  35. Sakata D, Yao C, Narumiya S. Prostaglandin E2, an immunoactivator. J Pharmacol Sci. 2010 ; 112(1) : 1-5. https://doi.org/10.1254/jphs.09R03CP
  36. Bannenberg GL. Therapeutic applicability of anti-inflammatory and proresolving polyunsaturated fatty acid-derived lipid mediators. Scientific World Journal. 2010 ; 10 : 676-712. https://doi.org/10.1100/tsw.2010.57
  37. Pratheeshkumar P, Kuttan G. Cardiospermum halicacabum inhibits Cyclophosphamide Induced Immunosupression and Oxidative Stress in Mice and also Regulates iNOS and COX-2 Gene Expression in LPS Stimulated Macrophages. Asian Pac J Cancer Prev. 2011 ; 11(5) : 1245-52.
  38. Qidwai T, Jamal F. Inducible nitric oxide synthase (iNOS) gene polymorphism and disease prevalence. Scand J Immunol. 2010 ; 72(5) : 375-87. https://doi.org/10.1111/j.1365-3083.2010.02458.x
  39. Choi RJ, Shin EM, Jung HA, Choi JS, Kim YS. Inhibitory effects of kaurenoic acid from Aralia continentalis on LPS-induced inflammatory response in RAW264.7 macrophages. Phytomedicine. 2011 ; Epub ahead of print.
  40. Bonvissuto G, Minutoli L, Morgia G, Bitto A, Polito F, Irrera N, Marini H, Squadrito F, Altavilla D. Effect of Serenoa repens, lyocopene, and selenium on proinflammatory $I_{\kappa}B-\alpha$ phenotype activation : an in vitro and in vivo comparison study. Urology. 2011 ; 77(1) : 248.e9-16. https://doi.org/10.1016/j.urology.2010.07.514

피인용 문헌

  1. 항염증 효능을 가진 단미 한약의 2001년 이후 실험 연구 결과에 대한 고찰 vol.25, pp.4, 2011, https://doi.org/10.6114/jkood.2012.25.4.012