Journal of Acupuncture Research

  • 제28권2호
  • /
  • Pages.125-131
  • /
  • 2011
  • /
  • 2586-288X(pISSN)
  • /
  • 2586-2898(eISSN)
대한침구의학회 (Korean Acupuncture & Moxibustion Medicine Society)
권호 표지

합환피(合歡皮) 약침액(藥鍼液)의 사람 피부아세포의 콜라게나제 활성 및 프로콜라겐 합성과 티로시나제 활성에 미치는 영향

Effects of Albizziae Cortex Pharmacopuncture Extracts on the Collagenase Activity and Procollagen Synthesis in HS68 Human Fibroblasts and Tyrosinase Activity

  • 임강현 (세명대학교 한의과대학 본초학교실)
  • Leem, Kang-Hyun (Dept. of Herbology, College of Oriental Medicine, Semyung University)
  • 투고 : 2011.03.11
  • 심사 : 2011.03.24
  • 발행 : 2011.04.20
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

목적 : 본 연구는 합환피약침액(合歡皮藥鍼液)이 사람 피부 섬유아세포의 콜라게나제 활성 및 프로콜라겐 합성에 미치는 영항과 티로시나제 활성에 미치는 효과를 측정하고자 실시하였다. 방법 : HS68 사람 정상 섬유아세포에 UVB 조사 후 합환피(合歡皮) 약침액(藥鍼液)가 type I procollagen 생성과 콜라게나제 효소활성에 미치는 효능과 티로시나제 효소활성에 미치는 효능을 평가하였다. 결과 : 합환피약침액(合歡皮藥鍼液)은 UVB 조사된 세포의 콜라게나제 효소활성을 통계적으로 유의하게 억제하였고, 티로시나제 활성을 통계적으로 유의하게 억제하였다. 그러나 티로시나제 억제활성의 정도는 미백효능으로 활용하기에 약간 약한 경향이 있었다. 결론 : 합환피약침액(合歡皮藥鍼液)의 콜라게나제 억제효능은 주름개선 약침치료에 활용이 가능할 것으로 생각된다.

키워드

참고문헌

  1. The Boncho-Hak Textbook Committee. Boncho- Hak. Seoul : Young-Lim Press. 2007 : 534-5.
  2. Chen JL, Chen TT. Chinese medical herbology and pharmacology. CA : Art of Medicine Press. 2004 : 768-9.
  3. Yaar M, Gilchrest BA. Cellular and molecular mechanisms of cutaneous aging. J Dermatol Surg Oncol. 1990 ; 16(10) : 915-22.
  4. Gelse K, Poschl E, Aigner T. Collagens- -structure, function, and biosynthesis. Adv Drug Deliv Rev. 2003 ; 55(12) : 1531-46. https://doi.org/10.1016/j.addr.2003.08.002
  5. Fisher GJ, Datta SC, Talwar HS, Wang ZQ, Varani J, Kang S, Voorhees JJ. Molecular basis of sun-induced premature skin ageing and retinoid antagonism. Nature. 1996 ; 379(6563) : 335-9. https://doi.org/10.1038/379335a0
  6. Shin JY, Hur W, Wang JS, Jang JW, Kim CW, Bae SH, Jang SK, Yang SH, Sung YC, Kwon OJ, Yoon SK. HCV core protein promotes liver fibrogenesis via up-regulation of CTGF with TGF-beta1. Exp Mol Med. 2005 ; 37(2) : 138-45. https://doi.org/10.1038/emm.2005.19
  7. Wei X, Liu Y, Xiao J, Wang Y. Protective effects of tea polysaccharides and polyphenols on skin. J Agric Food Chem. 2009 ; 57(17) : 7757-62. https://doi.org/10.1021/jf901340f
  8. Moon HJ, Lee SH, Ku MJ, Yu BC, Jeon MJ, Jeong SH, Stonik VA, Zvyagintseva TN, Ermakova SP, Lee YH. Fucoidan inhibits UVB-induced MMP-1 promoter expression and down regulation of type I procollagen synthesis in human skin fibroblasts. Eur J Dermatol. 2009 ; 19(2) : 129-34.
  9. Vanni A, Gastaldi D, Giunata G. Kinetic investigations on the double enzyme activity of the tyrosinase mushroom. Ann Chim. 1990 ; 80 : 35-60.
  10. Joshi PC, Carraro C, Pathak MA. Involvement of reactive oxygen species in the oxidation of tyrosine and dopa to melanin and in skin tanning. Biochem Biophys Res Commun. 1987 ; 142(1) : 265-74. https://doi.org/10.1016/0006-291X(87)90480-3
  11. Kim YH, Chung CB, Kim JG, Ko KI, Park SH, Kim JH et al. Anti-wrinkle activity of ziyuglycoside I isolated from a Sanguisorba officinalis root extract and its application as a cosmeceutical ingredient. Biosci Biotechnol Biochem. 2008 ; 72(2) : 303-11. https://doi.org/10.1271/bbb.70268
  12. Müller WEG. The Origin of Metazoan Complexity: Porifera as Integrated Animals. Integr Comp Biol. 2003 ; 43(1) : 3-10. https://doi.org/10.1093/icb/43.1.3
  13. Di Lullo GA, Sweeney SM, Korkko J, Ala- Kokko L, San Antonio JD. Mapping the ligandbinding sites and disease-associated mutations on the most abundant protein in the human, type I collagen. J Biol Chem. 2002 ; 277(6) : 4223-31. https://doi.org/10.1074/jbc.M110709200
  14. Sikorski ZE. Chemical and Functional Properties of Food Proteins. Boca Raton : CRC Press. 2001 : 242.
  15. Lei TC, Virador VM, Vieira WD, Hearing VJ. A melanocyte-keratinocyte coculture model to assess regulators of pigmentation in vitro. Anal Biochem. 2002 ; 305(2) : 260-8. https://doi.org/10.1006/abio.2002.5665